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Protein

Carbamoyl-phosphate synthase (glutamine-hydrolyzing)

Gene

BamMC406_1181

Organism
Burkholderia ambifaria (strain MC40-6)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.SAAS annotation

Cofactori

Mg2+SAAS annotation, Mn2+SAAS annotationNote: Binds 4 Mg(2+) or Mn2+ ions per subunit.SAAS annotation

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. arginine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesisSAAS annotation, Pyrimidine biosynthesisSAAS annotation

Keywords - Ligandi

MagnesiumSAAS annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation

Enzyme and pathway databases

BioCyciBAMB398577:GH38-1218-MONOMER.
UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase (glutamine-hydrolyzing)SAAS annotation (EC:6.3.5.5SAAS annotation)
Gene namesi
Ordered Locus Names:BamMC406_1181Imported
OrganismiBurkholderia ambifaria (strain MC40-6)Imported
Taxonomic identifieri398577 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001680 Componenti: Chromosome 1

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.SAAS annotation

Protein-protein interaction databases

STRINGi398577.BamMC406_1181.

Structurei

3D structure databases

ProteinModelPortaliB1YMV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG0458.
HOGENOMiHOG000234582.
KOiK01955.
OMAiAVFPFNK.
OrthoDBiEOG6J1DC6.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_A. CPSase_L_chain_A.
MF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1YMV8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRTDIKSI LIIGAGPIII GQACEFDYSG AQACKALREE GYKVVLVNSN
60 70 80 90 100
PATIMTDPNT ADVTYIEPIT WEVVARIIEK ERPDAILPTM GGQTALNCAL
110 120 130 140 150
DLHHHGVLEK FGVELIGASP EAIDKAEDRQ KFKDAMTKIG LGSAKSGIAH
160 170 180 190 200
SMEEATQVHA EIMAYTGGSG YPVVIRPSFT LGGSGGGIAY NREEFEEICK
210 220 230 240 250
RGLDLSPTRE LLIEESLLGW KEYEMEVVRD RADNCIIVCS IENLDPMGVH
260 270 280 290 300
TGDSITVAPA QTLTDKEYQI LRNASLAVLR EIGVDTGGSN VQFSINPKDG
310 320 330 340 350
RMVVIEMNPR VSRSSALASK ATGFPIAKVA AKLAVGYTLD ELKNEITGGQ
360 370 380 390 400
TPASFEPTID YVVTKIPRFA FEKFREADSR LTTQMKSVGE VMAIGRTFQE
410 420 430 440 450
SFQKALRGLE VGVDGLDEKS TDRDEIAIEI HEPGPDRIWY VGDAFRIGMT
460 470 480 490 500
AEEIFAETAI DPWFLAQIEQ IILKEKALSG RTLASLTFDE LRFLKQSGFS
510 520 530 540 550
DRRLAKLLGA TPEDVRKRRV ELNVRPVYKR VDTCAAEFAT KTAYMYSTYE
560 570 580 590 600
EECEAQPTTN KKIMVLGGGP NRIGQGIEFD YCCVHAALAM REDGYETIMV
610 620 630 640 650
NCNPETVSTD YDTSDRLYFE PLTLEDVLEI VDKEKPVGVI VQYGGQTPLK
660 670 680 690 700
LALDLEAHGV PIVGTSPDMI DAAEDRERFQ KLLQDLGLRQ PPNRTARAEE
710 720 730 740 750
EALALAAEIG YPLVVRPSYV LGGRAMEIVH EPRDLERYMR EAVKVSNDSP
760 770 780 790 800
VLLDRFLNDA IECDVDCICD GEAVFIGGVM EHIEQAGVHS GDSACSLPPY
810 820 830 840 850
SLSKETVAEL KRQTGAMAKA LNVVGLMNVQ FAIQQVPQAD GSKQDIIYVL
860 870 880 890 900
EVNPRASRTV PYVSKATSLP LAKIAARAMV GQKLAQQGVT KEVEPPYFSV
910 920 930 940 950
KEAVFPFVKF PTVDPVLGPE MRSTGEVMGV GQTFGEALFK SQLAAGSRLP
960 970 980 990 1000
ESGTVLLTVM DADKPKAVEV ARMLHDLGYP IVATKGTAAA IEAAGVPVKV
1010 1020 1030 1040 1050
VNKVKDGRPH IVDMIKNGEI ALVFTTVDET RQAIADSRSI RMSAQAHKVT
1060 1070 1080
YYTTMSGARA AVEGLRYLKD LEVYDLQGLH ARLN
Length:1,084
Mass (Da):118,534
Last modified:May 20, 2008 - v1
Checksum:i03C125233E06ED28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001025 Genomic DNA. Translation: ACB63672.1.
RefSeqiWP_012363549.1. NC_010551.1.
YP_001807888.1. NC_010551.1.

Genome annotation databases

EnsemblBacteriaiACB63672; ACB63672; BamMC406_1181.
KEGGibac:BamMC406_1181.
PATRICi19031760. VBIBurAmb82852_1213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001025 Genomic DNA. Translation: ACB63672.1.
RefSeqiWP_012363549.1. NC_010551.1.
YP_001807888.1. NC_010551.1.

3D structure databases

ProteinModelPortaliB1YMV8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi398577.BamMC406_1181.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB63672; ACB63672; BamMC406_1181.
KEGGibac:BamMC406_1181.
PATRICi19031760. VBIBurAmb82852_1213.

Phylogenomic databases

eggNOGiCOG0458.
HOGENOMiHOG000234582.
KOiK01955.
OMAiAVFPFNK.
OrthoDBiEOG6J1DC6.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.
BioCyciBAMB398577:GH38-1218-MONOMER.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_A. CPSase_L_chain_A.
MF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia ambifaria MC40-6."
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A.
    , Ramette A., Konstantinidis K., Tiedje J., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC40-6Imported.

Entry informationi

Entry nameiB1YMV8_BURA4
AccessioniPrimary (citable) accession number: B1YMV8
Entry historyi
Integrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: April 1, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.