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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Burkholderia ambifaria (strain MC40-6)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+SAAS annotation
  • Mn2+SAAS annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotation
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotationSAAS annotation, Pyrimidine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, MagnesiumSAAS annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotation
Ordered Locus Names:BamMC406_1181Imported
OrganismiBurkholderia ambifaria (strain MC40-6)Imported
Taxonomic identifieri398577 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000001680 Componenti: Chromosome 1

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliB1YMV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 335ATP-graspInterPro annotationAdd BLAST203
Domaini680 – 880ATP-graspInterPro annotationAdd BLAST201

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 410Carboxyphosphate synthetic domainUniRule annotationAdd BLAST410
Regioni947 – 1084Allosteric domainUniRule annotationAdd BLAST138

Sequence similaritiesi

Belongs to the CarB family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000234582.
KOiK01955.
OMAiAVFPFNK.
OrthoDBiPOG091H01IP.

Family and domain databases

CDDicd01424. MGS_CPS_II. 1 hit.
Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 1 hit.
3.40.50.1380. 1 hit.
HAMAPiMF_01210_A. CPSase_L_chain_A. 1 hit.
MF_01210_B. CPSase_L_chain_B. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR006275. CarbamoylP_synth_lsu.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR033937. MGS_CPS_CarB.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
PRINTSiPR00098. CPSASE.
SMARTiView protein in SMART
SM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.

Sequencei

Sequence statusi: Complete.

B1YMV8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKRTDIKSI LIIGAGPIII GQACEFDYSG AQACKALREE GYKVVLVNSN
60 70 80 90 100
PATIMTDPNT ADVTYIEPIT WEVVARIIEK ERPDAILPTM GGQTALNCAL
110 120 130 140 150
DLHHHGVLEK FGVELIGASP EAIDKAEDRQ KFKDAMTKIG LGSAKSGIAH
160 170 180 190 200
SMEEATQVHA EIMAYTGGSG YPVVIRPSFT LGGSGGGIAY NREEFEEICK
210 220 230 240 250
RGLDLSPTRE LLIEESLLGW KEYEMEVVRD RADNCIIVCS IENLDPMGVH
260 270 280 290 300
TGDSITVAPA QTLTDKEYQI LRNASLAVLR EIGVDTGGSN VQFSINPKDG
310 320 330 340 350
RMVVIEMNPR VSRSSALASK ATGFPIAKVA AKLAVGYTLD ELKNEITGGQ
360 370 380 390 400
TPASFEPTID YVVTKIPRFA FEKFREADSR LTTQMKSVGE VMAIGRTFQE
410 420 430 440 450
SFQKALRGLE VGVDGLDEKS TDRDEIAIEI HEPGPDRIWY VGDAFRIGMT
460 470 480 490 500
AEEIFAETAI DPWFLAQIEQ IILKEKALSG RTLASLTFDE LRFLKQSGFS
510 520 530 540 550
DRRLAKLLGA TPEDVRKRRV ELNVRPVYKR VDTCAAEFAT KTAYMYSTYE
560 570 580 590 600
EECEAQPTTN KKIMVLGGGP NRIGQGIEFD YCCVHAALAM REDGYETIMV
610 620 630 640 650
NCNPETVSTD YDTSDRLYFE PLTLEDVLEI VDKEKPVGVI VQYGGQTPLK
660 670 680 690 700
LALDLEAHGV PIVGTSPDMI DAAEDRERFQ KLLQDLGLRQ PPNRTARAEE
710 720 730 740 750
EALALAAEIG YPLVVRPSYV LGGRAMEIVH EPRDLERYMR EAVKVSNDSP
760 770 780 790 800
VLLDRFLNDA IECDVDCICD GEAVFIGGVM EHIEQAGVHS GDSACSLPPY
810 820 830 840 850
SLSKETVAEL KRQTGAMAKA LNVVGLMNVQ FAIQQVPQAD GSKQDIIYVL
860 870 880 890 900
EVNPRASRTV PYVSKATSLP LAKIAARAMV GQKLAQQGVT KEVEPPYFSV
910 920 930 940 950
KEAVFPFVKF PTVDPVLGPE MRSTGEVMGV GQTFGEALFK SQLAAGSRLP
960 970 980 990 1000
ESGTVLLTVM DADKPKAVEV ARMLHDLGYP IVATKGTAAA IEAAGVPVKV
1010 1020 1030 1040 1050
VNKVKDGRPH IVDMIKNGEI ALVFTTVDET RQAIADSRSI RMSAQAHKVT
1060 1070 1080
YYTTMSGARA AVEGLRYLKD LEVYDLQGLH ARLN
Length:1,084
Mass (Da):118,534
Last modified:May 20, 2008 - v1
Checksum:i03C125233E06ED28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001025 Genomic DNA. Translation: ACB63672.1.
RefSeqiWP_012363549.1. NC_010551.1.

Genome annotation databases

EnsemblBacteriaiACB63672; ACB63672; BamMC406_1181.
KEGGibac:BamMC406_1181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001025 Genomic DNA. Translation: ACB63672.1.
RefSeqiWP_012363549.1. NC_010551.1.

3D structure databases

ProteinModelPortaliB1YMV8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB63672; ACB63672; BamMC406_1181.
KEGGibac:BamMC406_1181.

Phylogenomic databases

HOGENOMiHOG000234582.
KOiK01955.
OMAiAVFPFNK.
OrthoDBiPOG091H01IP.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Family and domain databases

CDDicd01424. MGS_CPS_II. 1 hit.
Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 1 hit.
3.40.50.1380. 1 hit.
HAMAPiMF_01210_A. CPSase_L_chain_A. 1 hit.
MF_01210_B. CPSase_L_chain_B. 1 hit.
InterProiView protein in InterPro
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR006275. CarbamoylP_synth_lsu.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR033937. MGS_CPS_CarB.
IPR016185. PreATP-grasp_dom.
PfamiView protein in Pfam
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
PRINTSiPR00098. CPSASE.
SMARTiView protein in SMART
SM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiView protein in PROSITE
PS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiB1YMV8_BURA4
AccessioniPrimary (citable) accession number: B1YMV8
Entry historyiIntegrated into UniProtKB/TrEMBL: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 7, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.