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B1YLD8

- ENO_EXIS2

UniProt

B1YLD8 - ENO_EXIS2

Protein

Enolase

Gene

eno

Organism
Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

    Catalytic activityi

    2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei155 – 1551SubstrateUniRule annotation
    Binding sitei164 – 1641SubstrateUniRule annotation
    Active sitei205 – 2051Proton donorUniRule annotation
    Metal bindingi242 – 2421MagnesiumUniRule annotation
    Metal bindingi287 – 2871MagnesiumUniRule annotation
    Binding sitei287 – 2871SubstrateUniRule annotation
    Metal bindingi314 – 3141MagnesiumUniRule annotation
    Binding sitei314 – 3141SubstrateUniRule annotation
    Active sitei339 – 3391Proton acceptorUniRule annotation
    Binding sitei339 – 3391Substrate (covalent); in inhibited formUniRule annotation
    Binding sitei390 – 3901SubstrateUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. phosphopyruvate hydratase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciESIB262543:GHBP-2474-MONOMER.
    UniPathwayiUPA00109; UER00187.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
    Alternative name(s):
    2-phospho-D-glycerate hydro-lyaseUniRule annotation
    2-phosphoglycerate dehydrataseUniRule annotation
    Gene namesi
    Name:enoUniRule annotation
    Ordered Locus Names:Exig_2393
    OrganismiExiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15)
    Taxonomic identifieri262543 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillales Family XII. Incertae SedisExiguobacterium
    ProteomesiUP000001681: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation. Secreted UniRule annotation. Cell surface UniRule annotation
    Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

    GO - Cellular componenti

    1. cell surface Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-SubCell
    3. phosphopyruvate hydratase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 430430EnolasePRO_1000115864Add
    BLAST

    Proteomic databases

    PRIDEiB1YLD8.

    Interactioni

    Protein-protein interaction databases

    STRINGi262543.Exig_2393.

    Structurei

    3D structure databases

    ProteinModelPortaliB1YLD8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni366 – 3694Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the enolase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0148.
    HOGENOMiHOG000072173.
    KOiK01689.
    OMAiRSEIKGQ.
    OrthoDBiEOG65J589.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPiMF_00318. Enolase.
    InterProiIPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view]
    PANTHERiPTHR11902. PTHR11902. 1 hit.
    PfamiPF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001400. Enolase. 1 hit.
    PRINTSiPR00148. ENOLASE.
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01060. eno. 1 hit.
    PROSITEiPS00164. ENOLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1YLD8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSMITEIYAR EILDSRGNPT VEVEVYTEDG GFGRALVPSG ASTGEHEAVE    50
    LRDGDKSRYL GKGVLKAVDN VNEKLAPEII GYDVFDQAGI DKKMIDLDGT 100
    KNKGNFGANA ILGISMAAAR AAADELGLPL YTYLGGFNAK TLPTPMMNII 150
    NGGSHADNNV DFQEFMIMPV GAPTFKEALR MGAEIFHALK SVLSGMGLNT 200
    AVGDEGGFAP NLKSNEEAIT VILEAIEKAG YKAGEDVYLA MDVASSEFYD 250
    KAAGKYNLAG EGKVLSTEEL VEFYAQLVDK YPIISIEDGC DENDWDGHKL 300
    LTDRIGHKVQ LVGDDLFVTN TEKLAEGIEK GIANSILIKV NQIGTLTETF 350
    DAIEMAKKAG YTAVVSHRSG ETEDSTIADI AVATNAGQIK TGSLSRTDRI 400
    AKYNQLLRIE DMLSDVAVYD GIKSFYNLKK 430
    Length:430
    Mass (Da):46,328
    Last modified:May 20, 2008 - v1
    Checksum:iD332A59301988103
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001022 Genomic DNA. Translation: ACB61843.1.
    RefSeqiYP_001814860.1. NC_010556.1.

    Genome annotation databases

    EnsemblBacteriaiACB61843; ACB61843; Exig_2393.
    GeneIDi6172765.
    KEGGiesi:Exig_2393.
    PATRICi32138418. VBIExiSib53410_2431.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001022 Genomic DNA. Translation: ACB61843.1 .
    RefSeqi YP_001814860.1. NC_010556.1.

    3D structure databases

    ProteinModelPortali B1YLD8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 262543.Exig_2393.

    Proteomic databases

    PRIDEi B1YLD8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB61843 ; ACB61843 ; Exig_2393 .
    GeneIDi 6172765.
    KEGGi esi:Exig_2393.
    PATRICi 32138418. VBIExiSib53410_2431.

    Phylogenomic databases

    eggNOGi COG0148.
    HOGENOMi HOG000072173.
    KOi K01689.
    OMAi RSEIKGQ.
    OrthoDBi EOG65J589.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00187 .
    BioCyci ESIB262543:GHBP-2474-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPi MF_00318. Enolase.
    InterProi IPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view ]
    PANTHERi PTHR11902. PTHR11902. 1 hit.
    Pfami PF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001400. Enolase. 1 hit.
    PRINTSi PR00148. ENOLASE.
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsi TIGR01060. eno. 1 hit.
    PROSITEi PS00164. ENOLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17290 / JCM 13490 / 255-15.

    Entry informationi

    Entry nameiENO_EXIS2
    AccessioniPrimary (citable) accession number: B1YLD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3