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B1YKH4 (SYL_EXIS2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Exig_2275
OrganismExiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) [Complete proteome] [HAMAP]
Taxonomic identifier262543 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillales Family XII. Incertae SedisExiguobacterium

Protein attributes

Sequence length801 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 801801Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091318

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1YKH4 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: C99750E7764ED912

FASTA80191,651
        10         20         30         40         50         60 
MPYNHREIEP KWQARWEQDN AFVTTEDPEK EGFYALDMFP YPSGAGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LARMKRMQGY NVLHPMGWDA FGLPAEQYAL DTGNDPREFT AQNIETFKRQ LKDLGFSYDW 

       130        140        150        160        170        180 
DREINTTDPK YYKWTQWIFT KLYEQGLAFV DEVAVNWCPA LGTVLANEEV IDGLSERGNH 

       190        200        210        220        230        240 
PVVRVPMRQW VLKITEYADR LLEDLDELDW PESVKEMQRN WIGKSEGAEI DFTIDGHKKQ 

       250        260        270        280        290        300 
VTVFTTRPDT VFGATYLVLA PEHPFVTDIT TEDHEEAVKA YIASVQTKSD LERTDLAKEK 

       310        320        330        340        350        360 
TGVFTGAFAV NPISGERLPI WIADYVLATY GTGAIMAVPA HDERDHEFAK QFDLPIIEVV 

       370        380        390        400        410        420 
KGGNVDEVAY TGEGEHVNSQ MLDGLSKQEA IETIIAELET NQVGRKKITF RLRDWLFSRQ 

       430        440        450        460        470        480 
RYWGEPIPVV HMEDGTMKTL DKSELPLELP MIPEIKPSGT GESPLALAED WLDYTDPVTG 

       490        500        510        520        530        540 
LKGRRETNTM PQWGGSCWYY LRFIDPHNEE AIADPEKLKY WLPVDIYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLYD IGVVPTKEPF QKLYNQGMIL GENNEKMSKS RGNVINPDEI VKSHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD ASVSWSENGL DGARRFLDRV WRLFERTADI QDVTTVDADF ERVYHQTVKK 

       670        680        690        700        710        720 
VTEDFTNIQF NTGISQLMVF VNEANKQPVL PRHFLRGFIQ LLTPVAPHLG EELWEQLGFE 

       730        740        750        760        770        780 
DTLTYAAWPT FDETKLVSDT MEFVIQVNGK VRSKLVISME ATKEEIEALA FADEKTQEWI 

       790        800 
GDKTVRKVIV VPKKLINIVA N 

« Hide

References

[1]"Complete sequence of chromosome of Exiguobacterium sibiricum 255-15."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F. expand/collapse author list , Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T., Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 17290 / JCM 13490 / 255-15.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001022 Genomic DNA. Translation: ACB61727.1.
RefSeqYP_001814744.1. NC_010556.1.

3D structure databases

ProteinModelPortalB1YKH4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262543.Exig_2275.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB61727; ACB61727; Exig_2275.
GeneID6173101.
KEGGesi:Exig_2275.
PATRIC32138178. VBIExiSib53410_2311.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycESIB262543:GHBP-2355-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_EXIS2
AccessionPrimary (citable) accession number: B1YKH4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries