Reviewed, UniProtKB/Swiss-Prot B1YJ94 (NADE_EXIS2)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Exig_2023

Organism

Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) [Complete proteome] [HAMAP]

Taxonomic identifier

262543 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillales Family XII. Incertae Sedis › Exiguobacterium

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000118623
Regions
	Nucleotide binding	43 – 50	8	ATP By similarity
Sites
	Active site	45	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1YJ94-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: D2BBB29D4E9BE661	FASTA	271	30,059
	10 20 30 40 50 60 MQQEIIQVTG VKPVIDAEEE IKQRVQFLKE YLVHTGAKGL VLGISGGQDS SLAGRLCQIA 70 80 90 100 110 120 VEELRSETNR DYQFYAVRLP YGQQQDESDA QLALSFIRPD HALRVDIKPA VAASMASFEQ 130 140 150 160 170 180 ATGDVLSDFS KGNTKARERM KVQYDIAAHY GCLVVGTDHA AEFVTGFYTK HGDGACDLTP 190 200 210 220 230 240 LTGLNKRQGK QLLRQLQAPE GLIEKVPTAD LEDNQPGLPD EQALGMTYNE IDDYLEGKTI 250 260 270 SAESQAKLEA QYKRVGHKHH MPVSPLDTWW K

References

[1]

"Complete sequence of chromosome of Exiguobacterium sibiricum 255-15." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T., Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001022 Genomic DNA. Translation: ACB61475.1.
RefSeq	YP_001814492.1.
3D structure databases
SMR	B1YJ94. Positions 1-271.
ModBase	Search...
Genome annotation databases
GeneID	6175590.
GenomeReviews	Gene locus Exig_2023 in contig CP001022_GR.
KEGG	esi:Exig_2023.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	HLYEKAP.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_EXIS2

Accession

Primary (citable) accession number: B1YJ94

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	February 9, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)