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B1YIS7

- SYI_EXIS2

UniProt

B1YIS7 - SYI_EXIS2

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei551 – 5511Aminoacyl-adenylateUniRule annotation
    Binding sitei595 – 5951ATPUniRule annotation
    Metal bindingi881 – 8811ZincUniRule annotation
    Metal bindingi884 – 8841ZincUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciESIB262543:GHBP-2029-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Exig_1951
    OrganismiExiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15)
    Taxonomic identifieri262543 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillales Family XII. Incertae SedisExiguobacterium
    ProteomesiUP000001681: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 911911Isoleucine--tRNA ligasePRO_1000189166Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi262543.Exig_1951.

    Structurei

    3D structure databases

    ProteinModelPortaliB1YIS7.
    SMRiB1YIS7. Positions 1-911.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi592 – 5965"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1YIS7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEYKDTLLMM KTEFLMRGNL PKREPDMQAR WNEMNLYEAV QEKNAGKPTF    50
    ILHDGPPYAN GDIHMGHGLN KVLKDIVVRY KSMNGFRSPY VPGWDTHGLP 100
    IETALQKAGV DRKSMSVAEF RELCAKYALE QVDHQREQFK RLGVLGDYDN 150
    PYITLQPEFE AAQIRLFGDM ANKGYIYKGK KPVYWSPSSE SALAEAEIEY 200
    QDKRSAAIYV AFQVMDGKNI LEPTDHFVIW TTTPWTIPAN LGISVSGELT 250
    YARIEHEGKG YIVAETLVPE VIEALGWEGA TIGRVFDGAD FEYIKAKHPL 300
    YDRESLVMLG DHVTAEATGV VHTAPGHGED DFRIGQAYGL DVLCPVDDKG 350
    VMTAEAPGFE GLFYEDANKE IGVALEEAGA LLKLSFIKHS YPHDWRTKKP 400
    VIFRATPQWF ASIKDFRAEI LDEIKEVQWV PEWGETRLHN MFKDRGDWVI 450
    SRQRAWGVPL PIFYAEDGTE IVTPETIDHI ANLFAAHGSN VWYEREAVDL 500
    LPEGFTHPAS PNGIFKKETD IMDVWFDSGS SHAGVLATRP ELERPADLYL 550
    EGSDQYRGWF NSSLSTSVAT TGKAPYKAVV SHGFVLDGQG RKMSKSIGNT 600
    IAPIQIMQQF GAEILRLWVA SVDYQSDVRA SMDNFKQVSE SYRKIRNTVR 650
    FLLGNLDQFD HTTHRVAFKD LPESDRFMRT KLDQLVGKVK AAYDAYDFMA 700
    VYQLLHNFCV LDLSSFYLDY TKDILYIEKE DAPARRAVQT VMYDTVVTLL 750
    QLMAPVLPHT ADEAWEFVPG VETASIFLTD LPEAPEMTEE GLALIAKWNS 800
    FLVFRDDVLK ALEEARAEKL VGKTLEAKLL LAPNDETKAL LATIDHLEQL 850
    LQVSQIEFVA SAEKSYGTTG ITVLKADGEK CERCWTYSTE LGQDPAHPTL 900
    CPRCTEVVNS L 911
    Length:911
    Mass (Da):102,680
    Last modified:May 20, 2008 - v1
    Checksum:i715DFFE7B370694A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001022 Genomic DNA. Translation: ACB61403.1.
    RefSeqiYP_001814420.1. NC_010556.1.

    Genome annotation databases

    EnsemblBacteriaiACB61403; ACB61403; Exig_1951.
    GeneIDi6174770.
    KEGGiesi:Exig_1951.
    PATRICi32137522. VBIExiSib53410_1984.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001022 Genomic DNA. Translation: ACB61403.1 .
    RefSeqi YP_001814420.1. NC_010556.1.

    3D structure databases

    ProteinModelPortali B1YIS7.
    SMRi B1YIS7. Positions 1-911.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 262543.Exig_1951.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB61403 ; ACB61403 ; Exig_1951 .
    GeneIDi 6174770.
    KEGGi esi:Exig_1951.
    PATRICi 32137522. VBIExiSib53410_1984.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci ESIB262543:GHBP-2029-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 17290 / JCM 13490 / 255-15.

    Entry informationi

    Entry nameiSYI_EXIS2
    AccessioniPrimary (citable) accession number: B1YIS7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3