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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei551 – 5511Aminoacyl-adenylateUniRule annotation
Binding sitei595 – 5951ATPUniRule annotation
Metal bindingi881 – 8811ZincUniRule annotation
Metal bindingi884 – 8841ZincUniRule annotation
Metal bindingi901 – 9011ZincUniRule annotation
Metal bindingi904 – 9041ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciESIB262543:GHBP-2029-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Exig_1951
OrganismiExiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15)
Taxonomic identifieri262543 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillales Family XII. Incertae SedisExiguobacterium
ProteomesiUP000001681 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 911911Isoleucine--tRNA ligasePRO_1000189166Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi262543.Exig_1951.

Structurei

3D structure databases

ProteinModelPortaliB1YIS7.
SMRiB1YIS7. Positions 1-911.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi592 – 5965"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1YIS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYKDTLLMM KTEFLMRGNL PKREPDMQAR WNEMNLYEAV QEKNAGKPTF
60 70 80 90 100
ILHDGPPYAN GDIHMGHGLN KVLKDIVVRY KSMNGFRSPY VPGWDTHGLP
110 120 130 140 150
IETALQKAGV DRKSMSVAEF RELCAKYALE QVDHQREQFK RLGVLGDYDN
160 170 180 190 200
PYITLQPEFE AAQIRLFGDM ANKGYIYKGK KPVYWSPSSE SALAEAEIEY
210 220 230 240 250
QDKRSAAIYV AFQVMDGKNI LEPTDHFVIW TTTPWTIPAN LGISVSGELT
260 270 280 290 300
YARIEHEGKG YIVAETLVPE VIEALGWEGA TIGRVFDGAD FEYIKAKHPL
310 320 330 340 350
YDRESLVMLG DHVTAEATGV VHTAPGHGED DFRIGQAYGL DVLCPVDDKG
360 370 380 390 400
VMTAEAPGFE GLFYEDANKE IGVALEEAGA LLKLSFIKHS YPHDWRTKKP
410 420 430 440 450
VIFRATPQWF ASIKDFRAEI LDEIKEVQWV PEWGETRLHN MFKDRGDWVI
460 470 480 490 500
SRQRAWGVPL PIFYAEDGTE IVTPETIDHI ANLFAAHGSN VWYEREAVDL
510 520 530 540 550
LPEGFTHPAS PNGIFKKETD IMDVWFDSGS SHAGVLATRP ELERPADLYL
560 570 580 590 600
EGSDQYRGWF NSSLSTSVAT TGKAPYKAVV SHGFVLDGQG RKMSKSIGNT
610 620 630 640 650
IAPIQIMQQF GAEILRLWVA SVDYQSDVRA SMDNFKQVSE SYRKIRNTVR
660 670 680 690 700
FLLGNLDQFD HTTHRVAFKD LPESDRFMRT KLDQLVGKVK AAYDAYDFMA
710 720 730 740 750
VYQLLHNFCV LDLSSFYLDY TKDILYIEKE DAPARRAVQT VMYDTVVTLL
760 770 780 790 800
QLMAPVLPHT ADEAWEFVPG VETASIFLTD LPEAPEMTEE GLALIAKWNS
810 820 830 840 850
FLVFRDDVLK ALEEARAEKL VGKTLEAKLL LAPNDETKAL LATIDHLEQL
860 870 880 890 900
LQVSQIEFVA SAEKSYGTTG ITVLKADGEK CERCWTYSTE LGQDPAHPTL
910
CPRCTEVVNS L
Length:911
Mass (Da):102,680
Last modified:May 20, 2008 - v1
Checksum:i715DFFE7B370694A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001022 Genomic DNA. Translation: ACB61403.1.
RefSeqiWP_012370821.1. NC_010556.1.
YP_001814420.1. NC_010556.1.

Genome annotation databases

EnsemblBacteriaiACB61403; ACB61403; Exig_1951.
KEGGiesi:Exig_1951.
PATRICi32137522. VBIExiSib53410_1984.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001022 Genomic DNA. Translation: ACB61403.1.
RefSeqiWP_012370821.1. NC_010556.1.
YP_001814420.1. NC_010556.1.

3D structure databases

ProteinModelPortaliB1YIS7.
SMRiB1YIS7. Positions 1-911.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262543.Exig_1951.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB61403; ACB61403; Exig_1951.
KEGGiesi:Exig_1951.
PATRICi32137522. VBIExiSib53410_1984.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciESIB262543:GHBP-2029-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17290 / JCM 13490 / 255-15.

Entry informationi

Entry nameiSYI_EXIS2
AccessioniPrimary (citable) accession number: B1YIS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: May 27, 2015
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.