B1YHQ8 (PANC_EXIS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	Exig_1779

Organism

Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) [Complete proteome] [HAMAP]

Taxonomic identifier

262543 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillales Family XII. Incertae Sedis › Exiguobacterium

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Pantothenate synthetase HAMAP MF_00158		PRO_1000118152
Regions
	Nucleotide binding	26 – 33	8	ATP By similarity
	Nucleotide binding	142 – 145	4	ATP By similarity
	Nucleotide binding	179 – 182	4	ATP By similarity
Sites
	Active site	33	1	Proton donor By similarity
	Binding site	57	1	Beta-alanine By similarity
	Binding site	57	1	Pantoate By similarity
	Binding site	148	1	Pantoate By similarity
	Binding site	171	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1YHQ8 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 62C0B15678443217	FASTA	276	30,516
	10 20 30 40 50 60 MKIMQSVTQL REALAGQTSV GFIPTMGFLH EGHASLLEQA RQENDIVVLS IFVNPTQFGP 70 80 90 100 110 120 NEDLDRYPRD EQRDQQLAQA AGVDYLFYPT NDVMYPLDMA RVTVRSGDDV LCGTSRPGHF 130 140 150 160 170 180 DGVLTVVSKL FNIVQPTRAY FGLKDAQQLA LIEGYVTDYF VPVEIKRCPI IREADGLAKS 190 200 210 220 230 240 SRNVYLSETE RKQAPGIQQA LQQAKQALDA GTPLETVLEQ TRASLHFEGT TIDYVEAVAY 250 260 270 PTLGPVDATT DTILLAVAVQ FESARLIDNL LYTRGA

References

[1]

"Complete sequence of chromosome of Exiguobacterium sibiricum 255-15." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T., Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17290 / JCM 13490 / 255-15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001022 Genomic DNA. Translation: ACB61231.1.
RefSeq	YP_001814248.1. NC_010556.1.
3D structure databases
ProteinModelPortal	B1YHQ8.
ModBase	Search...
Protein-protein interaction databases
STRING	B1YHQ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6173865.
GenomeReviews	Gene locus Exig_1779 in contig CP001022_GR.
KEGG	esi:Exig_1779.
PATRIC	32137174. VBIExiSib53410_1810.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG428839.
OMA	LNMPIQI.
ProtClustDB	PRK00380.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR004821. Cyt_trans-rel. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01918.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. Cyt_tran_rel. 1 hit. TIGR00018. PanC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_EXIS2

Accession

Primary (citable) accession number: B1YHQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program