B1YHE3 (B1YHE3_EXIS2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2 HAMAP MF_00108
EC=2.7.7.60 HAMAP MF_00108

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2 HAMAP MF_00108
MEP cytidylyltransferase 2 HAMAP MF_00108

Gene names

Name:	ispD2 HAMAP MF_00108
Ordered Locus Names:	Exig_0189

Organism

Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) [Complete proteome] [HAMAP]

Taxonomic identifier

262543 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillales Family XII. Incertae Sedis › Exiguobacterium

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU003490
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU003490
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108 RuleBase RU004393

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393
Molecular function	Nucleotidyltransferase SAAS SAAS018294 HAMAP MF_00108 RuleBase RU004393 EMBL ACB59675.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	14	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	22	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	160	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	217	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	B1YHE3 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 8F1EE481D630108E	FASTA	237	26,410
	10 20 30 40 50 60 MIYAAILAGG KGTRMGNVDR PKQFLSIGER PIIVHTVEKF LLHEDFDQII IVTPTAWINY 70 80 90 100 110 120 TRDILEKYIG TDERIVITAG GSDRNESIMA AINWIENKNG LTDEDVIVTH DAVRPFLTHR 130 140 150 160 170 180 IIKENISTAL EYGACDTVIS AIDTIVASTD GKTISDIPVR DQMYQGQTPQ SFQITKLKNH 190 200 210 220 230 YEALSSDERA ILTDACKILL LKGEAVALVT GELFNIKVTT PYDLRIANAI LKEQIHQ

References

[1]

"Complete sequence of chromosome of Exiguobacterium sibiricum 255-15." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T., Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17290 / JCM 13490 / 255-15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001022 Genomic DNA. Translation: ACB59675.1.
RefSeq	YP_001812692.1. NC_010556.1.
3D structure databases
ProteinModelPortal	B1YHE3.
ModBase	Search...
Protein-protein interaction databases
STRING	B1YHE3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6174537.
GenomeReviews	Gene locus Exig_0189 in contig CP001022_GR.
KEGG	esi:Exig_0189.
PATRIC	32133899. VBIExiSib53410_0201.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	GELFNIK.
ProtClustDB	PRK00155.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1YHE3_EXIS2

Accession

Primary (citable) accession number: B1YHE3

Entry history

Integrated into UniProtKB/TrEMBL:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	December 14, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)