Reviewed, UniProtKB/Swiss-Prot B1YHD6 (KYNU_EXIS2)

Names and origin

Protein names

Recommended name:
    Kynureninase
    EC=3.7.1.3
Alternative name(s):
    L-kynurenine hydrolase

Gene names

Name:	kynU
Ordered Locus Names:	Exig_0182

Organism

Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) [Complete proteome] [HAMAP]

Taxonomic identifier

262543 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillales Family XII. Incertae Sedis › Exiguobacterium

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively By similarity.
Catalytic activity	L-kynurenine + H2O = anthranilate + L-alanine. L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1. Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the kynureninase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro tryptophan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	kynureninase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Kynureninase		PRO_0000357004
Regions
	Region	129 – 132	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	101	1	Pyridoxal phosphate; via amide nitrogen By similarity
	Binding site	102	1	Pyridoxal phosphate By similarity
	Binding site	210	1	Pyridoxal phosphate By similarity
	Binding site	213	1	Pyridoxal phosphate By similarity
	Binding site	235	1	Pyridoxal phosphate By similarity
	Binding site	264	1	Pyridoxal phosphate By similarity
	Binding site	292	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	236	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1YHD6-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 8BDA1A8D2351607D	FASTA	425	47,194
	10 20 30 40 50 60 MTLTAPRKHA IEQDQQDALA PYRNEFYLQE GSIYMDGNSL GLLSKRAEAT LLESLADWRE 70 80 90 100 110 120 LGIDGWMKGR HPWFDLSEKL AALNAPLVGG RADEVMVTGS TTVNLHQLVA TFFAPSGRRT 130 140 150 160 170 180 KILADSLTFP SDIYALQSQL RLRGLDPAEH LVQVESRDGR FLDEADIIAA MTDDIALIVL 190 200 210 220 230 240 PTVLYRSGQI LDMERLTREA HARGILIGFD GCHSVGAIPH AFHDWGVDFA YWCNYKHLNG 250 260 270 280 290 300 GPGTVGGLFV HERHFGTLPG LTGWFGSRKD KQFDMNHTMT PAENAAAFQI GTPHVLSLAP 310 320 330 340 350 360 QIGALELFAE VGIDAVRAKS LALTDYMMTL VDQELTAYGF VIGNPRDAKR RGAHLSLEHP 370 380 390 400 410 420 EAARICKALK AHQVIPDFRA PNIVRLAPVA LYNSFEDVYE VVSILKTIMD EKQYEQFKNE REVVA

References

[1]

"Complete sequence of chromosome of Exiguobacterium sibiricum 255-15." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T., Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001022 Genomic DNA. Translation: ACB59668.1.
RefSeq	YP_001812685.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6173872.
GenomeReviews	Gene locus Exig_0182 in contig CP001022_GR.
KEGG	esi:Exig_0182.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NFPTDVY.
Family and domain databases
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR010111. Kynureninase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR14084. Kynureninase. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
TIGRFAMs	TIGR01814. kynureninase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KYNU_EXIS2

Accession

Primary (citable) accession number: B1YHD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	May 20, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)