Reviewed, UniProtKB/Swiss-Prot B1YHD4 (T23O_EXIS2)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	Exig_0180

Organism

Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) [Complete proteome] [HAMAP]

Taxonomic identifier

262543 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillales Family XII. Incertae Sedis › Exiguobacterium

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Tryptophan 2,3-dioxygenase		PRO_0000360115
Regions
	Region	23 – 27	5	Substrate binding By similarity
	Region	48 – 52	5	Substrate binding By similarity
Sites
	Metal binding	237	1	Iron (heme axial ligand) By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	121	1	Heme By similarity
	Binding site	251	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1YHD4-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 3219A060D1E9FB58	FASTA	279	32,478
	10 20 30 40 50 60 MKQTDATNIE ESIQTDFKKD MSYGDYLQLD SLLTSQQRLS DHHDEMLFIV IHQTSELWMK 70 80 90 100 110 120 LILHEMTAAI QAIADDQLER SFKMLARVSK IQQQLIQSWS VLSTLTPAEY LEFRDSLGHS 130 140 150 160 170 180 SGFQSYQNRQ IEFSLGFKNA QMLRVYEHET ALFAQLNDDL QTPSIYDETV RAMHRRGLPI 190 200 210 220 230 240 DEAVLNRDVT QDWEADASVE AAWAIVYQDV EQYWDLYELG EKLLDIGSQQ QMWRFNHMST 250 260 270 VERIIGQKPG TGGSSGVSYL RRVLDHRFFP ELWSVRTKL

References

[1]

"Complete sequence of chromosome of Exiguobacterium sibiricum 255-15." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T., Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001022 Genomic DNA. Translation: ACB59666.1.
RefSeq	YP_001812683.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6174276.
GenomeReviews	Gene locus Exig_0180 in contig CP001022_GR.
KEGG	esi:Exig_0180.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MKLILHE.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_EXIS2

Accession

Primary (citable) accession number: B1YHD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	May 20, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)