Reviewed, UniProtKB/Swiss-Prot B1YH73 (GUAC_EXIS2)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	Exig_1681

Organism

Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) [Complete proteome] [HAMAP]

Taxonomic identifier

262543 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillales Family XII. Incertae Sedis › Exiguobacterium

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity.
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	GMP reductase HAMAP MF_01511		PRO_1000146135
Regions
	Nucleotide binding	205 – 228	24	NADP Potential
Sites
	Active site	176	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1YH73-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 366A2DB2B0AC8A5E	FASTA	328	36,074
	10 20 30 40 50 60 MMDVVFDYED IQLIPAKSIV GSRSECDTTV EFGGRRFKLP VVPANMQTII DEKIATFLAE 70 80 90 100 110 120 NGYFYIMHRF EPETRLNFVR EMQQRGLIAS ISVGVKTEEY TFIETLAQEG LTPEYITIDI 130 140 150 160 170 180 AHGHSEAVIR MIQHIKQILP ESFVIAGNVG TPEAVRELEH AGADATKVGI GPGKVCITKI 190 200 210 220 230 240 KTGFGTGGWQ LAALRWCAKA ASKPIIADGG IRTHGDIAKS VRFGATMVMI GSLFAGHEES 250 260 270 280 290 300 PGEMQEVDGL QVKEYFGSAS EFQKGEKKNV EGKKMFIEFK GSLSDTLTEM EQDLQSAISY 310 320 AGGNKLQAIR TVDYVVVKNS IFNGDKVY

References

[1]

"Complete sequence of chromosome of Exiguobacterium sibiricum 255-15." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T., Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001022 Genomic DNA. Translation: ACB61134.1.
RefSeq	YP_001814151.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6175487.
GenomeReviews	Gene locus Exig_1681 in contig CP001022_GR.
KEGG	esi:Exig_1681.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	NSRSECD.
Family and domain databases
HAMAP	MF_01511. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_EXIS2

Accession

Primary (citable) accession number: B1YH73

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)