B1YFI1 (SYR_EXIS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	Exig_2860

Organism

Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) [Complete proteome] [HAMAP]

Taxonomic identifier

262543 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillales Family XII. Incertae Sedis › Exiguobacterium

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm By similarity HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Arginine--tRNA ligase HAMAP MF_00123		PRO_1000095364
Regions
	Motif	121 – 131	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	B1YFI1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: C89298E007340BCF	FASTA	560	63,144
	10 20 30 40 50 60 MSYKQQYAQV LHDVIGDALS QEEIEQLIEQ PKHEDHGDLA FPCFQLAKAF RKAPMMIATE 70 80 90 100 110 120 LAEKIDNPLF SNVQAAGPYV NVFLNREVVS REIIKRVLEE QQAYGSSELN GKTIVTDFSS 130 140 150 160 170 180 PNIAKPFSMG HLRSTVIGNA LNQISRKKGY EVVGINHLGD WGTQFGKLMV AYNMWGKEED 190 200 210 220 230 240 VRSEPIKELL KLYVRFHEEA EENPGLEDEG RAWFKKLEQG DEQATALWTW FREVSLVEFS 250 260 270 280 290 300 RVYEMLGVSF DSLNGEAFYN DKMQHVIDLL EEKDLLVESE GAMVVDLEAE GMPPALIKKK 310 320 330 340 350 360 DGATLYATRD LAAAVYRLET YGFEQAFYVV GGEQALHFKQ LFAVLKKLGF ENVDGMHHVP 370 380 390 400 410 420 FGLIMKDGKK MSTRKGRIVL LEEVLQDAIN VAKQNIAEKN PNLANAEQTA REVGVGAVIF 430 440 450 460 470 480 HDLKNERMNN IEFDLEQMLK FEGETGPYVQ YTNARANSLL RKGAYDGSAF NGSADDYSWG 490 500 510 520 530 540 VVSMLNQFSA VIDRAFTRRE PSIISRYVLD LAQSFNKYYG QVRVLEDDAE KQSRLALVKS 550 560 VTIVLTEGLR LLGVGAPEEM

References

[1]

"Complete sequence of chromosome of Exiguobacterium sibiricum 255-15." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Kiss H., Chertkov O., Monk C., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Vishnivetskaya T., Rodrigues D.F., Gilichinsky D., Tiedje J., Richardson P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 17290 / JCM 13490 / 255-15.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001022 Genomic DNA. Translation: ACB62306.1.
RefSeq	YP_001815323.1. NC_010556.1.
3D structure databases
ProteinModelPortal	B1YFI1.
ModBase	Search...
Protein-protein interaction databases
STRING	B1YFI1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6172585.
GenomeReviews	Gene locus Exig_2860 in contig CP001022_GR.
KEGG	esi:Exig_2860.
PATRIC	32139396. VBIExiSib53410_2903.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695395.
OMA	FHEEAEK.
ProtClustDB	PRK12451.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001278. Arg-tRNA-synth_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01887.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. ArgS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_EXIS2

Accession

Primary (citable) accession number: B1YFI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program