B1YBN8 (ILVD_THENV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Tneu_1925

Organism

Thermoproteus neutrophilus (strain DSM 2338 / JCM 9278 / V24Sta) [Complete proteome] [HAMAP]

Taxonomic identifier

444157 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Thermoproteus

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089423
Sites
	Metal binding	124	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	196	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B1YBN8 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: E00426A63F6D6C9B	FASTA	561	59,606
	10 20 30 40 50 60 MVKIKVRSSA WYDGVDNASH RSYLRAVGFT EEDFAKPLVG VLAAWSELGP CNYHTLDLAR 70 80 90 100 110 120 YVKEGVKEAG GVGLTAPTIV VNDGINMGTP GMRYSLISRD LIADTIEAQF NAHGVDAWVG 130 140 150 160 170 180 IGGCDKTQPG IMMAMVRLDL PAVYLYGGTA EAGWLGEREL TIEDTFEAVG SYLAGKITLE 190 200 210 220 230 240 ELKRIEELSF PTYGTCQGLF TANTMAMLSE ALGLALLGSA SPPATSARRR AYAVASGRAV 250 260 270 280 290 300 LKAAELGVTP RRVVTYDAIY NAAVTLFATA GSTNAILHLL AIAHEAGVKF TLDDFDEISR 310 320 330 340 350 360 RVPVIAALRP AGPYAMQDLD RIGGVPRILK KLYKAGFLRP EALTVEGETI GKLLERWQPP 370 380 390 400 410 420 AVPEDGILYS VEKPYKPYSG IRILRGNLAP DGAVMKIGAA DKLKFEGTAK VYNGEAEAFK 430 440 450 460 470 480 AVAAGEIKPG DVVVIRYEGP KGAPGMPEML KVTAAIVGAG LGEAVALVTD GRFSGATRGI 490 500 510 520 530 540 MVGHVAPEAA VGGPIALVEN GDKIAIDGET GRITLQIPQE ELERRRKNWT PPPPKYSGGL 550 560 LAKYAALVQQ ADKGAVTTPP R

References

[1]

"Complete sequence of Thermoproteus neutrophilus V24Sta." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M., Saltikov C., House C.H., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2338 / JCM 9278 / V24Sta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001014 Genomic DNA. Translation: ACB40840.1.
RefSeq	YP_001795286.1. NC_010525.1.
3D structure databases
ProteinModelPortal	B1YBN8.
ModBase	Search...
Protein-protein interaction databases
STRING	B1YBN8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6164868.
GenomeReviews	Gene locus Tneu_1925 in contig CP001014_GR.
KEGG	tne:Tneu_1925.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	NMPGAMI.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_THENV

Accession

Primary (citable) accession number: B1YBN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program