B1YBM5 (PURL_THENV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Tneu_1912

Organism

Thermoproteus neutrophilus (strain DSM 2338 / JCM 9278 / V24Sta) [Complete proteome] [HAMAP]

Taxonomic identifier

444157 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Thermoproteus

Protein attributes

Sequence length	693 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 693	693	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_1000194839
Regions
	Nucleotide binding	91 – 102	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B1YBM5 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 88DA04AD6F86E01E	FASTA	693	74,555
	10 20 30 40 50 60 MALTRQELEL IRRGLGREPT AAELAFFTSH WSEHCSYKST RRWLRELPGS APWVVRGRGT 70 80 90 100 110 120 DAPLVEVAPG LYVSFKIESH NHPSAVDPYN GAATGVGGII RDILTVGATP VALLVNLHFG 130 140 150 160 170 180 PPEDPHARWI FSNVVKGISD YGNRVGVPVV GGETWFDEDF TYTPIVLATC VGVVEAEAVP 190 200 210 220 230 240 RGGVAPGDYL VVAGLGADRS GLGGSAFASK TLEGGEDLGA VQVADPLMGK KLIDVVREAG 250 260 270 280 290 300 RCVKFIKDLG GGGLATALAE LSSWFSLGIE FHLDKLHVRD RAAAPEELLI SETQERLIFV 310 320 330 340 350 360 VSPQDLPCLE AALRRYEVPY SIPGRFVEGG RVWVMWRGER VVDIPISLAD GAPEVLWPQE 370 380 390 400 410 420 PYQPPELPQL PEPPLEKALD LVLSSPNVAK KESIYMRFDF DVGVKTAVKP GEGDAAVLKL 430 440 450 460 470 480 YQRGQLGLVV KGDANPRYTF LDPRLGAANA FVKAYRNVAV VGGVPLAAVD SINVGSPERP 490 500 510 520 530 540 RVYWQFVQAV QGLREAAAEL EVPIVGGKVS LYNEYMGRPV KPTVAVVVLG RIDDVSKANR 550 560 570 580 590 600 AMWREGDRIF VWGVTRGEVG GSEYLKRVHG VVAGRPPAVD YGAERKIVDV VQSWLGRLTG 610 620 630 640 650 660 ATDVGVGGLA AALAKMAVNS GVGATVDVCR APSDVGRLDF LLFSESNGRF VAAGEEGPGV 670 680 690 AVGEAHGDVF EVRCGGTLLY KRRVEELRRL MLL

References

[1]

"Complete sequence of Thermoproteus neutrophilus V24Sta." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M., Saltikov C., House C.H., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2338 / JCM 9278 / V24Sta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001014 Genomic DNA. Translation: ACB40827.1.
RefSeq	YP_001795273.1. NC_010525.1.
3D structure databases
ProteinModelPortal	B1YBM5.
ModBase	Search...
Protein-protein interaction databases
STRING	B1YBM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6164920.
GenomeReviews	Gene locus Tneu_1912 in contig CP001014_GR.
KEGG	tne:Tneu_1912.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
ProtClustDB	PRK01213.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_THENV

Accession

Primary (citable) accession number: B1YBM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program