B1Y9D1 (ENDA_PYRNV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	Tneu_1435

Organism

Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / V24Sta) (Thermoproteus neutrophilus) [Complete proteome] [HAMAP]

Taxonomic identifier

444157 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Pyrobaculum › Pyrobaculum neutrophilum

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01833
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01833
Subunit structure	Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	tRNA-splicing endonuclease HAMAP-Rule MF_01833		PRO_1000188342
Sites
	Active site	120	1	By similarity
	Active site	128	1	By similarity
	Active site	159	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1Y9D1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: C82380BB0FBA252E	FASTA	183	20,350
	10 20 30 40 50 60 MSGVLRGLAV VVQDVELARA LYREGFYGRF LGYDKVKRDE VDKVSAPLIL ALYEALYLAE 70 80 90 100 110 120 RGRIKVVAED GAEIPPEKLA ELGRGMIRNF DDVYKIYKYF RDLGYVVKSG LKFGALFSVY 130 140 150 160 170 180 EKGPGIDHAP MVVVFLEPDR GISATDITRG GRLGHSVKKT FTLATVLRQT GEVVLLGFSW AKL

References

[1]

"Complete sequence of Thermoproteus neutrophilus V24Sta." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M., Saltikov C., House C.H., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2338 / JCM 9278 / V24Sta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001014 Genomic DNA. Translation: ACB40360.1.
RefSeq	YP_001794806.1. NC_010525.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	444157.Tneu_1435.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB40360; ACB40360; Tneu_1435.
GeneID	6164660.
KEGG	tne:Tneu_1435.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000107823.
KO	K01170.
OMA	GIDHAPM.
ProtClustDB	PRK09297.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
HAMAP	MF_01833. EndA_short.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR006676. tRNA_splic. IPR016442. tRNA_splic_arch_short. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. PF02778. tRNA_int_endo_N. 1 hit. [Graphical view]
PIRSF	PIRSF005285. tRNA_splic_archaea. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit. SSF55267. tRNA_int_endo_N. 1 hit.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENDA_PYRNV

Accession

Primary (citable) accession number: B1Y9D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program