B1Y963 (SYP_THENV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Tneu_1366

Organism

Thermoproteus neutrophilus (strain DSM 2338 / JCM 9278 / V24Sta) [Complete proteome] [HAMAP]

Taxonomic identifier

444157 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Thermoproteales › Thermoproteaceae › Thermoproteus

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Proline--tRNA ligase HAMAP MF_01571		PRO_1000215581

Sequences

	Sequence		Length	Mass (Da)
	B1Y963 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: C181341BBA32F10F	FASTA	487	56,465
	10 20 30 40 50 60 MELLRSARPH PKEKLKNVIE WFHWLLREAE LYDVRYPVKG AYVWRPYGMK LRRNVEELIR 70 80 90 100 110 120 RVHDETGHEE VLFPVFIPYE FFGKESQHIR GFEKEVFWVS KGGEGGERLV LRPTSETAIM 130 140 150 160 170 180 PMVKLWIQDY KDLPLRLYQI VSVFRAETKM THPMIRLREI SMFKEAHTVH VDREDAERQV 190 200 210 220 230 240 REAVEIYKRI FDEMCLAYMI NKRPDWDKFA GAEYTIAFDT VLPDGRTLQI GTAHYLGTNF 250 260 270 280 290 300 TRVFEVTYLD ADGTRKLAHT TSYGISERSI AAMLITHGDD GGTTLPPKLA PIQIAVVPIY 310 320 330 340 350 360 YSDEEMPLVM KFVEEVVAAL KGAGLRLHVD DRRDKTPGWK FYYWELKGVP LRLEVGKRDV 370 380 390 400 410 420 EKRQVVVTRR DTLEKYAVAL GELVDAVREL MKAVEDNLRR RAWEELRSKI VKVQSLEEAK 430 440 450 460 470 480 KAIKEGKVVE VPWSGDNQCG MKIQELLGAD ALGIPMDSEA SIGGYDARDL ACGERRAELW LRLSERY

References

[1]

"Complete sequence of Thermoproteus neutrophilus V24Sta." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M., Saltikov C., House C.H., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2338 / JCM 9278 / V24Sta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001014 Genomic DNA. Translation: ACB40292.1.
RefSeq	YP_001794738.1. NC_010525.1.
3D structure databases
ProteinModelPortal	B1Y963.
ModBase	Search...
Protein-protein interaction databases
STRING	B1Y963.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6164516.
GenomeReviews	Gene locus Tneu_1366 in contig CP001014_GR.
KEGG	tne:Tneu_1366.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG334108.
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_THENV

Accession

Primary (citable) accession number: B1Y963

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program