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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi54Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi58Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi86Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei88Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei155Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei180Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei184Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei388Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei392Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei498Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei547Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei574Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei817Substrate; via amide nitrogenUniRule annotation1
Binding sitei825Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei842Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciLCHO395495:G1GBL-1354-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Lcho_1343
OrganismiLeptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6))
Taxonomic identifieri395495 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesLeptothrix
Proteomesi
  • UP000001693 Componenti: Chromosome

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Tat-type signalUniRule annotationAdd BLAST29
ChainiPRO_500033186730 – 851Periplasmic nitrate reductaseUniRule annotationAdd BLAST822

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Proteomic databases

PRIDEiB1Y6A6

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi395495.Lcho_1343

Structurei

3D structure databases

ProteinModelPortaliB1Y6A6
SMRiB1Y6A6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 1004Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 224Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni248 – 252Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni267 – 269Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni524 – 525Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni741 – 750Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiANSMGTR
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
IPR019546 TAT_signal_bac_arc
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
TIGR01409 TAT_signal_seq, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B1Y6A6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSNRRDFLK AQALAASAAA AGIPIVVEAA NGTAAPKTAA DVAVRWDKAP
60 70 80 90 100
CRFCGTGCAV MVGVQEGKVV ATQGDPEAPV NRGLNCIKGY FLSKIMYGRD
110 120 130 140 150
RLQTPLLRKK NGVYDKEGDF VPVSWDEAFD IMAAKWKETL KTDGPTGIGM
160 170 180 190 200
FGSGQWTVWE GYAAAKLWKA GFRSNNLDPN ARHCMASAVT GFMRTFGIDE
210 220 230 240 250
PMGCYDDIEQ SDAFVLWGSN MAEMHPILWS RITDRRLSNP HVKVAVLSTY
260 270 280 290 300
EHRSFDLADQ AMIFKPQTDL AILNYIANYI ITNKKVNTEF VKKNINFKKG
310 320 330 340 350
ATDIGYGLRP GHALEKDATS NGYPGADGKP KGNPNDSTPI SFDEYAKFVS
360 370 380 390 400
EYTAEKVSEI SGVTVEQLKA LAELYADPKV KVVSYWTMGF NQHTRGTWAN
410 420 430 440 450
NMVYNIHLLT GKISQPGNGP FSLTGQPSAC GTAREVGTFA HRLPADMVVT
460 470 480 490 500
NPEHRHHAEE IWGLPEGTIP DKIGLHAVAQ SRALKDGKLK CYWVTTNNNM
510 520 530 540 550
QAGPNINGEI LPGWRNPKTF IVVSDPYPTA SAMAADLVLP AAMWVEKEGA
560 570 580 590 600
FGNAERRTQV WRQQVSAPGE ARSDLWQMME FSKRFKIEDV WTAELIAKKP
610 620 630 640 650
AVKGKTLFDV LFRNGKVDKY PLADLTKVNA KYIKDYTNDE SKAYGFYVQK
660 670 680 690 700
GLFEEYAEFG RGHGHDLAPF DVYHEVRGLR WPVVDGKETL WRFREGYDPY
710 720 730 740 750
VKKGEGVKFY GHKDGRANIF ALPYQPAAES PDKEYDLWLC TGRVLEHWHT
760 770 780 790 800
GTMTRRVPEL HRAVPEAQLF MHPDDAKARG LQRGMKVKIA SRRGEILLAV
810 820 830 840 850
ETKGRNKVPR GLVFVPFFDE GKLINKLTLD ATCPISKETD FKKCAVKVVR

A
Length:851
Mass (Da):94,789
Last modified:May 20, 2008 - v1
Checksum:i30900AA43A5B5A76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001013 Genomic DNA Translation: ACB33611.1
RefSeqiWP_012346373.1, NC_010524.1

Genome annotation databases

EnsemblBacteriaiACB33611; ACB33611; Lcho_1343
KEGGilch:Lcho_1343

Similar proteinsi

Entry informationi

Entry nameiNAPA_LEPCP
AccessioniPrimary (citable) accession number: B1Y6A6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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