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B1Y6A6 (NAPA_LEPCP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Lcho_1343
OrganismLeptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6)) [Complete proteome] [HAMAP]
Taxonomic identifier395495 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesLeptothrix

Protein attributes

Sequence length851 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929Tat-type signal Potential
Chain30 – 851822Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000331867

Regions

Domain44 – 100574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding511Iron-sulfur (4Fe-4S) By similarity
Metal binding541Iron-sulfur (4Fe-4S) By similarity
Metal binding581Iron-sulfur (4Fe-4S) By similarity
Metal binding861Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B1Y6A6 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 30900AA43A5B5A76

FASTA85194,789
        10         20         30         40         50         60 
MQSNRRDFLK AQALAASAAA AGIPIVVEAA NGTAAPKTAA DVAVRWDKAP CRFCGTGCAV 

        70         80         90        100        110        120 
MVGVQEGKVV ATQGDPEAPV NRGLNCIKGY FLSKIMYGRD RLQTPLLRKK NGVYDKEGDF 

       130        140        150        160        170        180 
VPVSWDEAFD IMAAKWKETL KTDGPTGIGM FGSGQWTVWE GYAAAKLWKA GFRSNNLDPN 

       190        200        210        220        230        240 
ARHCMASAVT GFMRTFGIDE PMGCYDDIEQ SDAFVLWGSN MAEMHPILWS RITDRRLSNP 

       250        260        270        280        290        300 
HVKVAVLSTY EHRSFDLADQ AMIFKPQTDL AILNYIANYI ITNKKVNTEF VKKNINFKKG 

       310        320        330        340        350        360 
ATDIGYGLRP GHALEKDATS NGYPGADGKP KGNPNDSTPI SFDEYAKFVS EYTAEKVSEI 

       370        380        390        400        410        420 
SGVTVEQLKA LAELYADPKV KVVSYWTMGF NQHTRGTWAN NMVYNIHLLT GKISQPGNGP 

       430        440        450        460        470        480 
FSLTGQPSAC GTAREVGTFA HRLPADMVVT NPEHRHHAEE IWGLPEGTIP DKIGLHAVAQ 

       490        500        510        520        530        540 
SRALKDGKLK CYWVTTNNNM QAGPNINGEI LPGWRNPKTF IVVSDPYPTA SAMAADLVLP 

       550        560        570        580        590        600 
AAMWVEKEGA FGNAERRTQV WRQQVSAPGE ARSDLWQMME FSKRFKIEDV WTAELIAKKP 

       610        620        630        640        650        660 
AVKGKTLFDV LFRNGKVDKY PLADLTKVNA KYIKDYTNDE SKAYGFYVQK GLFEEYAEFG 

       670        680        690        700        710        720 
RGHGHDLAPF DVYHEVRGLR WPVVDGKETL WRFREGYDPY VKKGEGVKFY GHKDGRANIF 

       730        740        750        760        770        780 
ALPYQPAAES PDKEYDLWLC TGRVLEHWHT GTMTRRVPEL HRAVPEAQLF MHPDDAKARG 

       790        800        810        820        830        840 
LQRGMKVKIA SRRGEILLAV ETKGRNKVPR GLVFVPFFDE GKLINKLTLD ATCPISKETD 

       850 
FKKCAVKVVR A 

« Hide

References

[1]"Complete sequence of Leptothrix cholodnii SP-6."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Emerson D., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51168 / LMG 8142 / SP-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001013 Genomic DNA. Translation: ACB33611.1.
RefSeqYP_001790376.1. NC_010524.1.

3D structure databases

ProteinModelPortalB1Y6A6.
SMRB1Y6A6. Positions 43-848.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395495.Lcho_1343.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB33611; ACB33611; Lcho_1343.
GeneID6160329.
KEGGlch:Lcho_1343.
PATRIC22392985. VBILepCho83238_1345.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMACACKVER.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycLCHO395495:GHYL-1361-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_LEPCP
AccessionPrimary (citable) accession number: B1Y6A6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families