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B1Y6A6

- NAPA_LEPCP

UniProt

B1Y6A6 - NAPA_LEPCP

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi58 – 581Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi86 – 861Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciLCHO395495:GHYL-1361-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:Lcho_1343
    OrganismiLeptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6))
    Taxonomic identifieri395495 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesLeptothrix
    ProteomesiUP000001693: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Tat-type signalUniRule annotationAdd
    BLAST
    Chaini30 – 851822Periplasmic nitrate reductasePRO_5000331867Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi395495.Lcho_1343.

    Structurei

    3D structure databases

    ProteinModelPortaliB1Y6A6.
    SMRiB1Y6A6. Positions 43-848.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini44 – 100574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiCACKVER.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B1Y6A6-1 [UniParc]FASTAAdd to Basket

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    MQSNRRDFLK AQALAASAAA AGIPIVVEAA NGTAAPKTAA DVAVRWDKAP    50
    CRFCGTGCAV MVGVQEGKVV ATQGDPEAPV NRGLNCIKGY FLSKIMYGRD 100
    RLQTPLLRKK NGVYDKEGDF VPVSWDEAFD IMAAKWKETL KTDGPTGIGM 150
    FGSGQWTVWE GYAAAKLWKA GFRSNNLDPN ARHCMASAVT GFMRTFGIDE 200
    PMGCYDDIEQ SDAFVLWGSN MAEMHPILWS RITDRRLSNP HVKVAVLSTY 250
    EHRSFDLADQ AMIFKPQTDL AILNYIANYI ITNKKVNTEF VKKNINFKKG 300
    ATDIGYGLRP GHALEKDATS NGYPGADGKP KGNPNDSTPI SFDEYAKFVS 350
    EYTAEKVSEI SGVTVEQLKA LAELYADPKV KVVSYWTMGF NQHTRGTWAN 400
    NMVYNIHLLT GKISQPGNGP FSLTGQPSAC GTAREVGTFA HRLPADMVVT 450
    NPEHRHHAEE IWGLPEGTIP DKIGLHAVAQ SRALKDGKLK CYWVTTNNNM 500
    QAGPNINGEI LPGWRNPKTF IVVSDPYPTA SAMAADLVLP AAMWVEKEGA 550
    FGNAERRTQV WRQQVSAPGE ARSDLWQMME FSKRFKIEDV WTAELIAKKP 600
    AVKGKTLFDV LFRNGKVDKY PLADLTKVNA KYIKDYTNDE SKAYGFYVQK 650
    GLFEEYAEFG RGHGHDLAPF DVYHEVRGLR WPVVDGKETL WRFREGYDPY 700
    VKKGEGVKFY GHKDGRANIF ALPYQPAAES PDKEYDLWLC TGRVLEHWHT 750
    GTMTRRVPEL HRAVPEAQLF MHPDDAKARG LQRGMKVKIA SRRGEILLAV 800
    ETKGRNKVPR GLVFVPFFDE GKLINKLTLD ATCPISKETD FKKCAVKVVR 850
    A 851
    Length:851
    Mass (Da):94,789
    Last modified:May 20, 2008 - v1
    Checksum:i30900AA43A5B5A76
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001013 Genomic DNA. Translation: ACB33611.1.
    RefSeqiWP_012346373.1. NC_010524.1.
    YP_001790376.1. NC_010524.1.

    Genome annotation databases

    EnsemblBacteriaiACB33611; ACB33611; Lcho_1343.
    GeneIDi6160329.
    KEGGilch:Lcho_1343.
    PATRICi22392985. VBILepCho83238_1345.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001013 Genomic DNA. Translation: ACB33611.1 .
    RefSeqi WP_012346373.1. NC_010524.1.
    YP_001790376.1. NC_010524.1.

    3D structure databases

    ProteinModelPortali B1Y6A6.
    SMRi B1Y6A6. Positions 43-848.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 395495.Lcho_1343.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB33611 ; ACB33611 ; Lcho_1343 .
    GeneIDi 6160329.
    KEGGi lch:Lcho_1343.
    PATRICi 22392985. VBILepCho83238_1345.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi CACKVER.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    BioCyci LCHO395495:GHYL-1361-MONOMER.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51168 / LMG 8142 / SP-6.

    Entry informationi

    Entry nameiNAPA_LEPCP
    AccessioniPrimary (citable) accession number: B1Y6A6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3