B1Y547 (DNAA_LEPCP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	Lcho_0001

Organism

Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6)) [Complete proteome] [HAMAP]

Taxonomic identifier

395495 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Leptothrix

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_1000121996
Regions
	Nucleotide binding	215 – 222	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B1Y547 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: A07DD34841A02041	FASTA	510	55,914
	10 20 30 40 50 60 MHTDLWERGC ERLAAELPEQ QFNTWIRPLP PADVTLAGDG VLVGLRVPNR FKLDWIRAQY 70 80 90 100 110 120 GSRIEATLSE LAGKPVRLEL SLLPRDAVAR AQGMAAAPAA QAGAAPQPTQ SPMAIGAALH 130 140 150 160 170 180 AAATARHDPQ SVVPTPGGSA NGRAAPRVGE PGGPVGTSTL PVAPTLSPAV SRGRLNPALT 190 200 210 220 230 240 FDTLVPGRAN QMARTAALHV VGAPGHMYNP LFIYGGVGLG KTHLIHAVGN ALIRDNPDAR 250 260 270 280 290 300 VLYLHAEQFI SDVVRNYQRK TFDELKAKYH SLDLLLIDDV QFFAGKDRTQ EEFFNAFEAL 310 320 330 340 350 360 LAKRAHIIMT SDTYPKGLVD IDERLTSRFD AGLTVAIEPP ELEMRVAILM KKSDAEGSRM 370 380 390 400 410 420 PEDVAFFVAK NVRANVRELE GALRKVLAYS RFSHKEISIN LAREALKDLL SIQNRQVSVE 430 440 450 460 470 480 NIQKTVADFY KIKIADMYSK KRPASIARPR QIAMYLAKEM TQKSLPEIGE LFGGRDHTTV 490 500 510 LHAVRKIGGE RQKNTELNQQ LHVLEQTLKG

References

[1]

"Complete sequence of Leptothrix cholodnii SP-6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Emerson D., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51168 / LMG 8142 / SP-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001013 Genomic DNA. Translation: ACB32277.1.
RefSeq	YP_001789042.1. NC_010524.1.
3D structure databases
ProteinModelPortal	B1Y547.
ModBase	Search...
Protein-protein interaction databases
STRING	B1Y547.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6160507.
GenomeReviews	Gene locus Lcho_0001 in contig CP001013_GR.
KEGG	lch:Lcho_0001.
PATRIC	22390263. VBILepCho83238_0001.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG695666.
OMA	WPLCLRR.
ProtClustDB	PRK00149.
Enzyme and pathway databases
BioCyc	LCHO395495:LCHO_0001-MONOMER.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_LEPCP

Accession

Primary (citable) accession number: B1Y547

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program