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B1Y500 (BIOF_LEPCP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
8-amino-7-oxononanoate synthase
Short name=AONS
EC=2.3.1.47
Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name=7-KAP synthase
Short name=KAPA synthase
8-amino-7-ketopelargonate synthase
Gene names
Name:bioF
Ordered Locus Names:Lcho_3642
OrganismLeptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6)) [Complete proteome] [HAMAP]
Taxonomic identifier395495 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Leptothrix ›

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide By similarity. HAMAP-Rule MF_01693

Catalytic activity

Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]. HAMAP-Rule MF_01693

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis. HAMAP-Rule MF_01693

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.

Ontologies

Keywords
   Biological processBiotin biosynthesis
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processbiotin biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular_function8-amino-7-oxononanoate synthase activity

Inferred from electronic annotation. Source: HAMAP

pyridoxal phosphate binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4084088-amino-7-oxononanoate synthase HAMAP-Rule MF_01693
PRO_0000381014

Regions

Region119 – 1202Pyridoxal phosphate binding By similarity
Region215 – 2184Pyridoxal phosphate binding By similarity
Region246 – 2494Pyridoxal phosphate binding By similarity

Sites

Binding site201Substrate By similarity
Binding site1441Substrate By similarity
Binding site1901Pyridoxal phosphate By similarity
Binding site3721Substrate By similarity

Amino acid modifications

Modified residue2491N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
B1Y500 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: DBED1D18A9D0B03F

FASTA40843,162
        10         20         30         40         50         60 
MLIEHLNRQL REREAQSLRR RRRIAETPCA PHQRVSLDGQ TASEARELLA FCSNDYLGLA 

        70         80         90        100        110        120 
NHPALIGALA EGARLWGAGA GASHLISGHT RAHAALEDTL AEWAAPNIPG AKALSFCTGY 

       130        140        150        160        170        180 
MANLALLTAL GDAEATLFAD KLNHASLVDG ALLAKAKLQR YAHRQFDVLE RQLAACTTPI 

       190        200        210        220        230        240 
KLIVTDAVFS MDGDLAELDQ LLSLAERFDA WLVIDDAHGF GVLGPKGRGS LAHFGLRSER 

       250        260        270        280        290        300 
LILMGTLGKA AGLGGAFVAA HPSVIDWLVQ SARAYIYTTA APPAIAHALS ASLALIAGDE 

       310        320        330        340        350        360 
GEARRQHLRE LIAALRCQLA ALIAAQPQLG WRLAESDTAI QPLIVGSNDS ALALAAALDA 

       370        380        390        400 
QGLWVPAIRP PTVPVGTARL RITLSAAHSQ ADVTRLVAAL ATTARELA

« Hide

References

[1]"Complete sequence of Leptothrix cholodnii SP-6."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Emerson D., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51168 / LMG 8142 / SP-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001013 Genomic DNA. Translation: ACB35896.1.
RefSeqYP_001792661.1. NC_010524.1.

3D structure databases

ProteinModelPortalB1Y500.
ModBaseSearch...

Protein-protein interaction databases

STRING395495.Lcho_3642.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB35896; ACB35896; Lcho_3642.
GeneID6162679.
KEGGlch:Lcho_3642.
PATRIC22397715. VBILepCho83238_3678.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0156.
HOGENOMHOG000221021.
KOK00652.
OMADFYAITQ.
ProtClustDBPRK05958.

Enzyme and pathway databases

BioCycLCHO395495:GHYL-3690-MONOMER.
UniPathwayUPA00078.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_01693. BioF_aminotrans_2.
InterProIPR022834. Amino_oxononanoate_synth.
IPR004839. Aminotransferase_I/II.
IPR004723. BioF.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMsTIGR00858. bioF. 1 hit.
PROSITEPS00599. AA_TRANSFER_CLASS_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBIOF_LEPCP
AccessionPrimary (citable) accession number: B1Y500
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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