B1Y3P8 (B1Y3P8_LEPCP) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061
Short name=CMK HAMAP MF_00061
EC=2.7.1.148 HAMAP MF_00061

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase HAMAP MF_00061

Gene names

Name:	ispE HAMAP MF_00061
Ordered Locus Names:	Lcho_3497

Organism

Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6)) [Complete proteome] [HAMAP] EMBL ACB35751.1

Taxonomic identifier

395495 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Leptothrix

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily. HAMAP MF_00061

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00061
Ligand	ATP-binding HAMAP MF_00061 Nucleotide-binding
Molecular function	Kinase HAMAP MF_00061 EMBL ACB35751.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	95 – 105	11	ATP By similarity HAMAP MF_00061
Sites
	Active site	13	1	By similarity HAMAP MF_00061
	Active site	137	1	By similarity HAMAP MF_00061

Sequences

	Sequence		Length	Mass (Da)
	B1Y3P8 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 1C624078A10D3770	FASTA	291	30,982
	10 20 30 40 50 60 MITALYDLLA PAKLNLFLHV IGRRADGYHL LQSVFRLIDW CDTLHLELRR NGQISRSDLG 70 80 90 100 110 120 PALPADDLCL RAARALQTAS GTPLGVHISI DKKVPWGAGL GGGSSDAATT LIGLNRLWRL 130 140 150 160 170 180 DWSREQLATL ALQLGADVPF FVGGRNAWVE GIGEKLTPIE LPGAAYAVVK PEVAIPTAAI 190 200 210 220 230 240 FSSPLLSRDT SPAIVADFLA DTKSFGKNDL QRSAQAHSAE VSQALSLLEH HVGASRMSGS 250 260 270 280 290 GSAVFACAGL APGASAKSEG WQQVLATLPQ TWTARWCHGL DDHPLSGWSK D

References

[1]

"Complete sequence of Leptothrix cholodnii SP-6." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Emerson D., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51168 / LMG 8142 / SP-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001013 Genomic DNA. Translation: ACB35751.1.
RefSeq	YP_001792516.1. NC_010524.1.
3D structure databases
ProteinModelPortal	B1Y3P8.
ModBase	Search...
Protein-protein interaction databases
STRING	B1Y3P8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6160012.
GenomeReviews	Gene locus Lcho_3497 in contig CP001013_GR.
KEGG	lch:Lcho_3497.
PATRIC	22397413. VBILepCho83238_3528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B1Y3P8_LEPCP

Accession

Primary (citable) accession number: B1Y3P8

Entry history

Integrated into UniProtKB/TrEMBL:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)