B1XY56 (SYE_LEPCP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Lcho_1690

Organism

Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6)) [Complete proteome] [HAMAP]

Taxonomic identifier

395495 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Leptothrix

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000367701
Regions
	Motif	14 – 24	11	"HIGH" region HAMAP MF_00022_B
	Motif	246 – 250	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	249	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1XY56 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: D27466157F32878E	FASTA	468	52,622
	10 20 30 40 50 60 MTQVSTPVRT RFAPSPTGFI HLGNIRSALY PWAFARATGG TFVLRIEDTD VERSSQAAVD 70 80 90 100 110 120 VIVEGMKWLG LDHDEGPFFQ MQRMARYKEV LAQLVARGQV YPCYMSMTEL DALRERQMAN 130 140 150 160 170 180 KEKPRYDGTW RPEPGKVLPA VPEGVQPVLR FRTPQGGSVI WDDKVKGRIE ISNDELDDLV 190 200 210 220 230 240 IARPDGTPTY NFCVVVDDID MAITHVIRGD DHVNNTPRQI HIFRALGQEP PVYAHLPTVL 250 260 270 280 290 300 NEQGEKMSKR HGAKPVTQYR EEGFLADAVV NYLARLGWSH GDDEIFSRAQ LIEWFNLDHL 310 320 330 340 350 360 GKSAGQYDEA KLRWVNSQHM KLCDNGVLAG LVAEQFARRE LLVPVDERLA RMCALFKDRC 370 380 390 400 410 420 STTVELADWL SMYFVPIQAS EQDLAAHVTD AVRPALQTLR DKLAALASWD KASISLCIKE 430 440 450 460 TIATHALKMP LLAVPVRVLV TGRAQTPSVD AVLELFQQEI VLKRLQSV

References

[1]

"Complete sequence of Leptothrix cholodnii SP-6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Emerson D., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51168 / LMG 8142 / SP-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001013 Genomic DNA. Translation: ACB33957.1.
RefSeq	YP_001790722.1. NC_010524.1.
3D structure databases
ProteinModelPortal	B1XY56.
ModBase	Search...
Protein-protein interaction databases
STRING	B1XY56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6161547.
GenomeReviews	Gene locus Lcho_1690 in contig CP001013_GR.
KEGG	lch:Lcho_1690.
PATRIC	22393699. VBILepCho83238_1700.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	IEWFNLD.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	LCHO395495:LCHO_1690-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_LEPCP

Accession

Primary (citable) accession number: B1XY56

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program