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B1XXY5 (SYL_LEPCP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Lcho_0479
OrganismLeptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6)) [Complete proteome] [HAMAP]
Taxonomic identifier395495 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesLeptothrix

Protein attributes

Sequence length887 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 887887Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091333

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_00049
Motif644 – 6485"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6471ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1XXY5 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 36C42A1C68EF7FDD

FASTA88797,733
        10         20         30         40         50         60 
MNEKYDPAAI ESAAHAQWVA ADAYRVDENA RDSQGQLKPK FYACSMLPYP SGKLHMGHVR 

        70         80         90        100        110        120 
NYTINDMLTR QLRMKGMNVL MPMGWDAFGL PAENAALKNS VPPEKWTREN IATMKGQMLA 

       130        140        150        160        170        180 
MGLAIDWSRE VATCDPTYYK WNQWLFLKML EAGIAERRTQ VVNWDPVDQT VLANEQVIDG 

       190        200        210        220        230        240 
RGWRSGALVE KREIPGYYLN IVKYADELLG AVANPEDPNY LSGWPERVRL MQENWIGKSE 

       250        260        270        280        290        300 
GVRFAFPHQI AGADGELIQG GKLYVFTTRA DTIMGVTFCA VAPEHPLAAH AAATNPALAA 

       310        320        330        340        350        360 
FIAECAHGGT TEAELATQEK KGLPTGLFVT HPLTGAQVEV WVGNYVLMSY GDGAVMGVPA 

       370        380        390        400        410        420 
HDERDFAFAK KYGISIRQVV QAEGQTFSLD GWADWYGDKQ RAVCVNSGLL DGLPYKEAVS 

       430        440        450        460        470        480 
KVAELVGAQG LGEKKTTWRL RDWGISRQRY WGTPIPIIHC DDCGSVPVPE KDLPVVLPID 

       490        500        510        520        530        540 
CVPDGSGNPL KKRTDFLNVA CPCCGKPAQR ETDTMDTFVD SSWYFMRYCD ARNSEQMVAG 

       550        560        570        580        590        600 
GTDYWMPMDQ YIGGIEHAIL HLLYARFWTK VMRDLGLVKV NEPFTKLLTQ GMVLNHIYSR 

       610        620        630        640        650        660 
RTDKGGIEYF WPHEVENVFD AGGKVTGAKL KSDGSAVDYG GIGTMSKSKN NGVDPQDLIN 

       670        680        690        700        710        720 
QYGADTARLF VMFASPPEQT LEWNDAGVEG AHRFLKRVWG FGVKQAELLK GATEVGAELS 

       730        740        750        760        770        780 
GDAKALRREV HLVLRQVSYD YERMQYNTVV SGSMKLLNAL EAYKHDGSAG SAAVLREGYS 

       790        800        810        820        830        840 
VLLRGLYPAC PHITHHLWSE LGYAAKLGDL LDAPWPEVVE AALVQDEIEL VLQVNGKTRG 

       850        860        870        880 
SIKVPAAADK AAIEAAAAAS AEVAKFADGK PIRKLIVVPG RLVNVVV 

« Hide

References

[1]"Complete sequence of Leptothrix cholodnii SP-6."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Lykidis A., Emerson D., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51168 / LMG 8142 / SP-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001013 Genomic DNA. Translation: ACB32754.1.
RefSeqYP_001789519.1. NC_010524.1.

3D structure databases

ProteinModelPortalB1XXY5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395495.Lcho_0479.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB32754; ACB32754; Lcho_0479.
GeneID6162097.
KEGGlch:Lcho_0479.
PATRIC22391243. VBILepCho83238_0486.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBCLSK2799920.

Enzyme and pathway databases

BioCycLCHO395495:GHYL-484-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LEPCP
AccessionPrimary (citable) accession number: B1XXY5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries