B1XXI5 (LPXD_LEPCP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Lcho_2840

Organism

Leptothrix cholodnii (strain ATCC 51168 / LMG 8142 / SP-6) (Leptothrix discophora (strain SP-6)) [Complete proteome] [HAMAP]

Taxonomic identifier

395495 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Leptothrix

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000127681
Sites
	Active site	242	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1XXI5 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 1361A32208B39D20	FASTA	342	35,281
	10 20 30 40 50 60 MQAALHELHA ALGGELLGDP SLVIRRIGPL ASADAETISF VSNARYRAQL AQTAAACVIV 70 80 90 100 110 120 APALAEDAAQ RGAAIVTPDP YHYFARLTQW WAARLRVAPP SGVHPSAVVA ADVRLGEGVS 130 140 150 160 170 180 VGPLTVIEAG AVLGDGVVIA SQCHIGAGVQ IGAQTRLAPH VTLMPGTRLG QRCLLHGGVV 190 200 210 220 230 240 IGADGFGFAP HQGRWEKIEQ LGGVVVGDDV EIGANTCIDR GALDDTVIGE GVKLDNLIQI 250 260 270 280 290 300 GHNVQIGAHS AMAGCAGVAG SARIGRGCTV GGGAIVLGHL ELADGVHISA ASVVMRSIKQ 310 320 330 340 PGQYSGVFPI DDNASWEKNA ATLRQLHTLR DRLRTLEKKS SP

References

[1]

"Complete sequence of Leptothrix cholodnii SP-6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Lykidis A., Emerson D., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51168 / LMG 8142 / SP-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001013 Genomic DNA. Translation: ACB35105.1.
RefSeq	YP_001791870.1. NC_010524.1.
3D structure databases
ProteinModelPortal	B1XXI5.
ModBase	Search...
Protein-protein interaction databases
STRING	B1XXI5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6163108.
GenomeReviews	Gene locus Lcho_2840 in contig CP001013_GR.
KEGG	lch:Lcho_2840.
PATRIC	22396087. VBILepCho83238_2870.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	CFVGKNT.
Enzyme and pathway databases
BioCyc	LCHO395495:LCHO_2840-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 5 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_LEPCP

Accession

Primary (citable) accession number: B1XXI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program