Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B1XW11 (SYQ_POLNS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:Pnec_1440
OrganismPolynucleobacter necessarius subsp. necessarius (strain STIR1) [Complete proteome] [HAMAP]
Taxonomic identifier452638 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter

Protein attributes

Sequence length590 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 590590Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_1000095496

Regions

Motif55 – 6511"HIGH" region HAMAP-Rule MF_00126
Motif299 – 3035"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site3021ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1XW11 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 72A5635BB5F03842

FASTA59067,311
        10         20         30         40         50         60 
MSQDSKPSKA NAGAVVEPSN FLRQIIDHDL ASGAFSQRTN LAGEAIPSII TRFPPEPNGY 

        70         80         90        100        110        120 
LHIGHAKSIC LNFGLASDYN NQPGGARCNM RLDDTNPVKE DVEYADSILD AVKWLGFDWG 

       130        140        150        160        170        180 
THLYHASDYF DRLYEFAEIL IQSGKAYVDS QSADDIHTNR GNFGQAGKNS PFRDRTPEEN 

       190        200        210        220        230        240 
LQLFRDMRDG KFKDGEHVLR LKIDMAHPNI VMRDPVVYRI RHTDHHRTGS KWCIYPLYDF 

       250        260        270        280        290        300 
THCISDALEN ISHSICTLEF ENNRPLYDWI VNSLKELGVF KDPVPHQHEF ARLNLTYTIT 

       310        320        330        340        350        360 
SKRKLLQLVE EKHVEGWDDP RMPTIVGIRR RGYTPESIRL FCERIGVSKA DSWIDMSTLD 

       370        380        390        400        410        420 
QALRDDLEVR APRATAVLKP LKLVVENFDA PTKEACSAPR HPNYPEWGNR EFNFTRELWI 

       430        440        450        460        470        480 
EADDFMQEPI KGFFRLYPPI DDQPGSRVRL RHGFVVECTG FETDAQGNVT QVNVTHFPDS 

       490        500        510        520        530        540 
KSGTPGSNNY KVKGNIHWIS AAEAIPAEVR LYDHLFTDPY PDSGDKNFLD AINPNSKQTI 

       550        560        570        580        590 
SAYLEPCMKD AKAEDRFQFE RHGYFVADQS DSKPGKPIFN RAVGLKDSWK 

« Hide

References

[1]"Complete sequence of Polynucleobacter necessarius STIR1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Hahn M., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: STIR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001010 Genomic DNA. Translation: ACB44538.1.
RefSeqYP_001798152.1. NC_010531.1.

3D structure databases

ProteinModelPortalB1XW11.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING452638.Pnec_1440.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB44538; ACB44538; Pnec_1440.
GeneID6183594.
KEGGpne:Pnec_1440.
PATRIC22972109. VBIPolNec8289_1653.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAKSDSWID.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycPNEC452638:GI4T-1439-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_POLNS
AccessionPrimary (citable) accession number: B1XW11
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries