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B1XV63 (MDH_POLNS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:Pnec_1065
OrganismPolynucleobacter necessarius subsp. necessarius (strain STIR1) [Complete proteome] [HAMAP]
Taxonomic identifier452638 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01517

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Malate dehydrogenase HAMAP-Rule MF_01517
PRO_1000191627

Regions

Nucleotide binding12 – 187NAD By similarity
Nucleotide binding132 – 1343NAD By similarity

Sites

Active site1901Proton acceptor By similarity
Binding site951Substrate By similarity
Binding site1011Substrate By similarity
Binding site1081NAD By similarity
Binding site1151NAD By similarity
Binding site1341Substrate By similarity
Binding site1651Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B1XV63 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 318BDD3C44157720

FASTA32935,546
        10         20         30         40         50         60 
MAKAPMRVAV TGAAGQIGYS LLFRIANGDL LGKDQPVILQ LLEIPDEKAQ KALAGVMMEL 

        70         80         90        100        110        120 
EDCAFPLLAG MTAHSDPMTA FKDIDVALLV GARPRGPGME RKDLLSANAQ IFTAQGKALN 

       130        140        150        160        170        180 
VVAKKTVKVL VVGNPANTNA YIAMKSAPDI PAKNFTAMLR LDHNRALSQL ANKLNKPVAD 

       190        200        210        220        230        240 
IEKLVVWGNH SPTMYPDYRF ATIDGKSVKD SINDAAWNKD VFIPTVGKRG AAIIEARGLS 

       250        260        270        280        290        300 
SAASAANAAI DHIHDWVLGT NGKWVTMGIP SKGEYDIPAE VIYGFPVVCE NGEYKMIEGL 

       310        320 
EIDEFSRERM THTLNELLEE QAGVKHLLS 

« Hide

References

[1]"Complete sequence of Polynucleobacter necessarius STIR1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Hahn M., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: STIR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001010 Genomic DNA. Translation: ACB44240.1.
RefSeqYP_001797854.1. NC_010531.1.

3D structure databases

ProteinModelPortalB1XV63.
SMRB1XV63. Positions 3-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING452638.Pnec_1065.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB44240; ACB44240; Pnec_1065.
GeneID6183484.
KEGGpne:Pnec_1065.
PATRIC22971244. VBIPolNec8289_1226.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
HOGENOMHOG000220953.
KOK00024.
OMACALRDEV.
OrthoDBEOG6PP9Q2.

Enzyme and pathway databases

BioCycPNEC452638:GI4T-1079-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01517. Malate_dehydrog_2.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_POLNS
AccessionPrimary (citable) accession number: B1XV63
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families