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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Polynucleobacter necessarius subsp. necessarius (strain STIR1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi487 – 4871MagnesiumUniRule annotation
Metal bindingi493 – 4931MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciPNEC452638:GI4T-823-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Pnec_0817
OrganismiPolynucleobacter necessarius subsp. necessarius (strain STIR1)
Taxonomic identifieri452638 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter
ProteomesiUP000006582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 722722Polyribonucleotide nucleotidyltransferasePRO_1000192482Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi452638.Pnec_0817.

Structurei

3D structure databases

ProteinModelPortaliB1XUJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KHUniRule annotationAdd
BLAST
Domaini623 – 69169S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1XUJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMFKKAVKS FQWGTYQVTM ETGEIARQAG GAVIVNVDDT VVMGTVVASK
60 70 80 90 100
SAKPGQSFFP LTVDYLEKTY AAGKIPGGFF RREGRPSEGE TLISRLIDRP
110 120 130 140 150
LRPLFPEGFL NEVQVVVHVL SINPDVPSDI PALIAASAAL AVSSIPFAGP
160 170 180 190 200
VGAARVGYAN GQYLLNPTRT EQATSELDLI VAGTQAAVLM VESEANQLSE
210 220 230 240 250
EVMLGAVVYG HDQMQTAINA INDLVCEAGK PEWDWTAAPK DEPFIAKVTA
260 270 280 290 300
LAEAPLREAY QIRQKGARSD KLKEISKEVM AKLSEEGDVD AVAVSDIMFE
310 320 330 340 350
IEAKIVRSQI LNGEPRIDGR DTRTVRPIEI RNGVLPRTHG SALFTRGETQ
360 370 380 390 400
ALVVATLGTA RDEQIIDVLE GEYRDRFMFH YNMPPFATGE TGRVGSPKRR
410 420 430 440 450
EIGHGRLAKR ALIPVLPSAE DFAYSIRVVS EITESNGSSS MASVCGGCLA
460 470 480 490 500
MMDAGVPVKA HVAGVAMGLI LDGNRFAVLT DILGDEDHLG DMDFKVAGTA
510 520 530 540 550
NGITALQMDI KVQGITKEIM QVALAQAKEG RLHILSKMQE AMGSVRTELS
560 570 580 590 600
AHAPRMVSFK IHPDKIREVI GKGGATIQAL TKETGCSIDI KDDGTVTIAS
610 620 630 640 650
TSAEGMAEAK ARIEGITAEA EVGKIYEGPV VKLLEFGALV NILPGKDGLL
660 670 680 690 700
HISEISNERV KEVKDYLAEG QVVRVKLLAA DERGRLRLSL KAAMADEGGT
710 720
IAPLAGAAEV ATEAAPATGE SA
Length:722
Mass (Da):77,109
Last modified:May 20, 2008 - v1
Checksum:i4C1FEAFDE52CE654
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001010 Genomic DNA. Translation: ACB44022.1.
RefSeqiWP_012357787.1. NC_010531.1.
YP_001797636.1. NC_010531.1.

Genome annotation databases

EnsemblBacteriaiACB44022; ACB44022; Pnec_0817.
KEGGipne:Pnec_0817.
PATRICi22970670. VBIPolNec8289_0952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001010 Genomic DNA. Translation: ACB44022.1.
RefSeqiWP_012357787.1. NC_010531.1.
YP_001797636.1. NC_010531.1.

3D structure databases

ProteinModelPortaliB1XUJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi452638.Pnec_0817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB44022; ACB44022; Pnec_0817.
KEGGipne:Pnec_0817.
PATRICi22970670. VBIPolNec8289_0952.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciPNEC452638:GI4T-823-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: STIR1.

Entry informationi

Entry nameiPNP_POLNS
AccessioniPrimary (citable) accession number: B1XUJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: May 27, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.