B1XTK6 (FABH_POLNS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.180

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	Pnec_0405

Organism

Polynucleobacter necessarius (strain STIR1) [Complete proteome] [HAMAP]

Taxonomic identifier

452638 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Polynucleobacter

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm By similarity HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: InterPro beta-ketoacyl-acyl-carrier-protein synthase III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000187887
Regions
	Region	256 – 260	5	ACP-binding By similarity
Sites
	Active site	122	1	By similarity
	Active site	255	1	By similarity
	Active site	285	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1XTK6 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 9CB0D4226CD6CC7A	FASTA	328	34,612
	10 20 30 40 50 60 MSIFARVAGT GSYLPELRLT NQDLVERLAK TGLETSDEWI KTRSGISARH FAAENELTSD 70 80 90 100 110 120 LAVRAAEAAL SSTGINSADL DLIILATSTP DHLGGFPSTA CVVQDKLGAH TSCAAFDVQA 130 140 150 160 170 180 VCAGFTYALA VADAFIRSGS YKKVLVIGAE TFSRILNFQD RGTCVLFGDG AGAVVLEASK 190 200 210 220 230 240 EAGILSTVMH ADGSQRDILC VPGRAGNGEV RGSPFMTMDG QAVFKLAVKV LEQVAHEALD 250 260 270 280 290 300 KAKLKPEQID WLVPHQANIR IMEGTAKKMG MSMDKVIVTV HEHGNTSAAS IPLALDSGVR 310 320 SGQIKRGQHL LLEGVGGGFA WGAVAIKY

References

[1]

"Complete sequence of Polynucleobacter necessarius STIR1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Hahn M., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: STIR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001010 Genomic DNA. Translation: ACB43683.1.
RefSeq	YP_001797297.1. NC_010531.1.
3D structure databases
ProteinModelPortal	B1XTK6.
ModBase	Search...
Protein-protein interaction databases
STRING	B1XTK6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6184264.
GenomeReviews	Gene locus Pnec_0405 in contig CP001010_GR.
KEGG	pne:Pnec_0405.
PATRIC	22969689. VBIPolNec8289_0473.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649927.
OMA	VIGSEVY.
ProtClustDB	CLSK2323581.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_POLNS

Accession

Primary (citable) accession number: B1XTK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program