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B1XT72

- SYL_POLNS

UniProt

B1XT72 - SYL_POLNS

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Polynucleobacter necessarius subsp. necessarius (strain STIR1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei648 – 6481ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPNEC452638:GI4T-263-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:Pnec_0254
    OrganismiPolynucleobacter necessarius subsp. necessarius (strain STIR1)
    Taxonomic identifieri452638 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter
    ProteomesiUP000006582: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 890890Leucine--tRNA ligasePRO_1000091342Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi452638.Pnec_0254.

    Structurei

    3D structure databases

    ProteinModelPortaliB1XT72.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi645 – 6495"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OMAiPVIHCPK.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1XT72-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKDYDYRSI EAAAQADWEG AQVYQVAENA VDAQGKKKSK YYACSMLPYP    50
    SGKLHMGHVR NYTINDVMAR QLRMQGYNVL MPMGWDAFGM PAENAAIQNK 100
    VPPAKWTYDN IAYMKKQMAA MGLAIDWSRE VATCNPDYYR WNQWLFLKML 150
    EKGIAYRKTQ VVNWDPIDQT VLANEQVIDG RGWRSGALVE KREIPGYYFN 200
    ITAYAEPLLS GLDGLGWPER VKTMQQNWIG KSRGVRFAFK HEIADDYGNF 250
    IQDGLLYVFT TRADTIMGVT FCAVAAEHPL ATKAAANNSA LAAFIEKCKT 300
    GSVIEADLAT QEKEGMFTGL YVTHPLTYEP VPVWVGNYVL MSYGDGAVMG 350
    VPAHDERDFA FALKYELPIK QVIALKDESP MFNATCWEDW YAQKDGVVCF 400
    NSAQFDGLSH EEAVSAVAKA LEKLGIGDIK TSFRLRDWGI SRQRYWGTPI 450
    PIIHCGDESN PGCGAVPVPE ADLPVVLPED CVPDGSGNPL NKRADFLSVK 500
    CPKCGKPARR ETDTMDTFVD SSWYFMRYTG PNAKTMVDER NEYWMPMDQY 550
    IGGIEHAILH LLYARFWTKI MRDLNLITFD EPFQNLLTQG MVLNETYYSE 600
    DASGKKTWLN PLDVELDLDE KGRPKGAKLI GDTLNTPVVV GGVEKMSKSK 650
    NNGVDPQALI DEYGADTARL FVMFAAPPEQ QLEWSGAGVD GASRFLRRVW 700
    MYFSGQASAL RDASDSLPSN LNDAEKELRR EVHTILKQAN FDYQRRQYNT 750
    VVSAAMKMLN ILEPIKLDQN AAISAPVLRE CLSILLRVLY PVVPHLTHVL 800
    WKEVGYAKTM GPLLDAPWPT VDEATLVQTE ITLMLQINGK LRGDIKVPAD 850
    ANKEQVEALA LQSEPAQKAL NGGAPKKVIV VPGRLVNIVA 890
    Length:890
    Mass (Da):99,540
    Last modified:May 20, 2008 - v1
    Checksum:i2CCD3CC373DE5E60
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001010 Genomic DNA. Translation: ACB43549.1.
    RefSeqiYP_001797163.1. NC_010531.1.

    Genome annotation databases

    EnsemblBacteriaiACB43549; ACB43549; Pnec_0254.
    GeneIDi6184601.
    KEGGipne:Pnec_0254.
    PATRICi22969351. VBIPolNec8289_0304.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001010 Genomic DNA. Translation: ACB43549.1 .
    RefSeqi YP_001797163.1. NC_010531.1.

    3D structure databases

    ProteinModelPortali B1XT72.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 452638.Pnec_0254.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB43549 ; ACB43549 ; Pnec_0254 .
    GeneIDi 6184601.
    KEGGi pne:Pnec_0254.
    PATRICi 22969351. VBIPolNec8289_0304.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OMAi PVIHCPK.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci PNEC452638:GI4T-263-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: STIR1.

    Entry informationi

    Entry nameiSYL_POLNS
    AccessioniPrimary (citable) accession number: B1XT72
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3