Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Polynucleobacter necessarius subsp. necessarius (strain STIR1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei648 – 6481ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPNEC452638:GI4T-263-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Pnec_0254
OrganismiPolynucleobacter necessarius subsp. necessarius (strain STIR1)
Taxonomic identifieri452638 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter
ProteomesiUP000006582 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 890890Leucine--tRNA ligasePRO_1000091342Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi452638.Pnec_0254.

Structurei

3D structure databases

ProteinModelPortaliB1XT72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi645 – 6495"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1XT72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKDYDYRSI EAAAQADWEG AQVYQVAENA VDAQGKKKSK YYACSMLPYP
60 70 80 90 100
SGKLHMGHVR NYTINDVMAR QLRMQGYNVL MPMGWDAFGM PAENAAIQNK
110 120 130 140 150
VPPAKWTYDN IAYMKKQMAA MGLAIDWSRE VATCNPDYYR WNQWLFLKML
160 170 180 190 200
EKGIAYRKTQ VVNWDPIDQT VLANEQVIDG RGWRSGALVE KREIPGYYFN
210 220 230 240 250
ITAYAEPLLS GLDGLGWPER VKTMQQNWIG KSRGVRFAFK HEIADDYGNF
260 270 280 290 300
IQDGLLYVFT TRADTIMGVT FCAVAAEHPL ATKAAANNSA LAAFIEKCKT
310 320 330 340 350
GSVIEADLAT QEKEGMFTGL YVTHPLTYEP VPVWVGNYVL MSYGDGAVMG
360 370 380 390 400
VPAHDERDFA FALKYELPIK QVIALKDESP MFNATCWEDW YAQKDGVVCF
410 420 430 440 450
NSAQFDGLSH EEAVSAVAKA LEKLGIGDIK TSFRLRDWGI SRQRYWGTPI
460 470 480 490 500
PIIHCGDESN PGCGAVPVPE ADLPVVLPED CVPDGSGNPL NKRADFLSVK
510 520 530 540 550
CPKCGKPARR ETDTMDTFVD SSWYFMRYTG PNAKTMVDER NEYWMPMDQY
560 570 580 590 600
IGGIEHAILH LLYARFWTKI MRDLNLITFD EPFQNLLTQG MVLNETYYSE
610 620 630 640 650
DASGKKTWLN PLDVELDLDE KGRPKGAKLI GDTLNTPVVV GGVEKMSKSK
660 670 680 690 700
NNGVDPQALI DEYGADTARL FVMFAAPPEQ QLEWSGAGVD GASRFLRRVW
710 720 730 740 750
MYFSGQASAL RDASDSLPSN LNDAEKELRR EVHTILKQAN FDYQRRQYNT
760 770 780 790 800
VVSAAMKMLN ILEPIKLDQN AAISAPVLRE CLSILLRVLY PVVPHLTHVL
810 820 830 840 850
WKEVGYAKTM GPLLDAPWPT VDEATLVQTE ITLMLQINGK LRGDIKVPAD
860 870 880 890
ANKEQVEALA LQSEPAQKAL NGGAPKKVIV VPGRLVNIVA
Length:890
Mass (Da):99,540
Last modified:May 20, 2008 - v1
Checksum:i2CCD3CC373DE5E60
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001010 Genomic DNA. Translation: ACB43549.1.
RefSeqiYP_001797163.1. NC_010531.1.

Genome annotation databases

EnsemblBacteriaiACB43549; ACB43549; Pnec_0254.
KEGGipne:Pnec_0254.
PATRICi22969351. VBIPolNec8289_0304.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001010 Genomic DNA. Translation: ACB43549.1.
RefSeqiYP_001797163.1. NC_010531.1.

3D structure databases

ProteinModelPortaliB1XT72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi452638.Pnec_0254.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB43549; ACB43549; Pnec_0254.
KEGGipne:Pnec_0254.
PATRICi22969351. VBIPolNec8289_0304.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPNEC452638:GI4T-263-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: STIR1.

Entry informationi

Entry nameiSYL_POLNS
AccessioniPrimary (citable) accession number: B1XT72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 1, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.