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B1XT72 (SYL_POLNS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pnec_0254
OrganismPolynucleobacter necessarius subsp. necessarius (strain STIR1) [Complete proteome] [HAMAP]
Taxonomic identifier452638 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter

Protein attributes

Sequence length890 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 890890Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091342

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_00049
Motif645 – 6495"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6481ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B1XT72 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 2CCD3CC373DE5E60

FASTA89099,540
        10         20         30         40         50         60 
MSKDYDYRSI EAAAQADWEG AQVYQVAENA VDAQGKKKSK YYACSMLPYP SGKLHMGHVR 

        70         80         90        100        110        120 
NYTINDVMAR QLRMQGYNVL MPMGWDAFGM PAENAAIQNK VPPAKWTYDN IAYMKKQMAA 

       130        140        150        160        170        180 
MGLAIDWSRE VATCNPDYYR WNQWLFLKML EKGIAYRKTQ VVNWDPIDQT VLANEQVIDG 

       190        200        210        220        230        240 
RGWRSGALVE KREIPGYYFN ITAYAEPLLS GLDGLGWPER VKTMQQNWIG KSRGVRFAFK 

       250        260        270        280        290        300 
HEIADDYGNF IQDGLLYVFT TRADTIMGVT FCAVAAEHPL ATKAAANNSA LAAFIEKCKT 

       310        320        330        340        350        360 
GSVIEADLAT QEKEGMFTGL YVTHPLTYEP VPVWVGNYVL MSYGDGAVMG VPAHDERDFA 

       370        380        390        400        410        420 
FALKYELPIK QVIALKDESP MFNATCWEDW YAQKDGVVCF NSAQFDGLSH EEAVSAVAKA 

       430        440        450        460        470        480 
LEKLGIGDIK TSFRLRDWGI SRQRYWGTPI PIIHCGDESN PGCGAVPVPE ADLPVVLPED 

       490        500        510        520        530        540 
CVPDGSGNPL NKRADFLSVK CPKCGKPARR ETDTMDTFVD SSWYFMRYTG PNAKTMVDER 

       550        560        570        580        590        600 
NEYWMPMDQY IGGIEHAILH LLYARFWTKI MRDLNLITFD EPFQNLLTQG MVLNETYYSE 

       610        620        630        640        650        660 
DASGKKTWLN PLDVELDLDE KGRPKGAKLI GDTLNTPVVV GGVEKMSKSK NNGVDPQALI 

       670        680        690        700        710        720 
DEYGADTARL FVMFAAPPEQ QLEWSGAGVD GASRFLRRVW MYFSGQASAL RDASDSLPSN 

       730        740        750        760        770        780 
LNDAEKELRR EVHTILKQAN FDYQRRQYNT VVSAAMKMLN ILEPIKLDQN AAISAPVLRE 

       790        800        810        820        830        840 
CLSILLRVLY PVVPHLTHVL WKEVGYAKTM GPLLDAPWPT VDEATLVQTE ITLMLQINGK 

       850        860        870        880        890 
LRGDIKVPAD ANKEQVEALA LQSEPAQKAL NGGAPKKVIV VPGRLVNIVA 

« Hide

References

[1]"Complete sequence of Polynucleobacter necessarius STIR1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Hahn M., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: STIR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001010 Genomic DNA. Translation: ACB43549.1.
RefSeqYP_001797163.1. NC_010531.1.

3D structure databases

ProteinModelPortalB1XT72.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING452638.Pnec_0254.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB43549; ACB43549; Pnec_0254.
GeneID6184601.
KEGGpne:Pnec_0254.
PATRIC22969351. VBIPolNec8289_0304.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPNEC452638:GI4T-263-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_POLNS
AccessionPrimary (citable) accession number: B1XT72
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries