Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B1XSN1 (FMT_POLNS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methionyl-tRNA formyltransferase

EC=2.1.2.9
Gene names
Name:fmt
Ordered Locus Names:Pnec_1777
OrganismPolynucleobacter necessarius subsp. necessarius (strain STIR1) [Complete proteome] [HAMAP]
Taxonomic identifier452638 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP-Rule MF_00182

Catalytic activity

10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP-Rule MF_00182

Sequence similarities

Belongs to the Fmt family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functionmethionyl-tRNA formyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Methionyl-tRNA formyltransferase HAMAP-Rule MF_00182
PRO_1000098425

Regions

Region124 – 1274Tetrahydrofolate (THF) binding By similarity

Sequences

Sequence LengthMass (Da)Tools
B1XSN1 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 5699A912ECC23449

FASTA33235,891
        10         20         30         40         50         60 
MKIVFAGTPE FAAQAVRAIH AAGHEIVLAF TQPDRRAGRG MHLQASPVKE FALQKNIPIL 

        70         80         90        100        110        120 
QPETLRRTSA DPQKKAQAEE AYKSLSAIEF DAMVVVAYGL ILPQEILDIT EKPGRHGSFN 

       130        140        150        160        170        180 
IHASLLPRWR GAAPIQRAIE AGDAKTGVCI MQMDAGLDTG DTVLVVELDI ARDETSASLH 

       190        200        210        220        230        240 
DRLAGLGADL IVNALDVLQQ GKTMIRSPQA IKGITYAEKI LKSEAEIDWT LSAQEIDQRI 

       250        260        270        280        290        300 
RAFNPFPGAS SKLNGYAIKL WNSRLADSQA FSMVGGAGDV LEFGRDGAYI QCGEGVLEVL 

       310        320        330 
EMQKPGGKKM AAKACIQPIG AGEKLLHFQL KE 

« Hide

References

[1]"Complete sequence of Polynucleobacter necessarius STIR1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Kim E., Hahn M., Richardson P.
Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: STIR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001010 Genomic DNA. Translation: ACB44830.1.
RefSeqYP_001798444.1. NC_010531.1.

3D structure databases

ProteinModelPortalB1XSN1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING452638.Pnec_1777.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB44830; ACB44830; Pnec_1777.
GeneID6183844.
KEGGpne:Pnec_1777.
PATRIC22972892. VBIPolNec8289_2039.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0223.
HOGENOMHOG000261177.
KOK00604.
OMAAESTIDW.
OrthoDBEOG6B09WV.
ProtClustDBCLSK2324156.

Enzyme and pathway databases

BioCycPNEC452638:GI4T-1773-MONOMER.

Family and domain databases

Gene3D3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPMF_00182. Formyl_trans.
InterProIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
IPR001555. GART_AS.
IPR015518. Met_tRNA_Form_TA-like.
[Graphical view]
PANTHERPTHR11138. PTHR11138. 1 hit.
PfamPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMSSF50486. SSF50486. 1 hit.
SSF53328. SSF53328. 1 hit.
TIGRFAMsTIGR00460. fmt. 1 hit.
PROSITEPS00373. GART. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFMT_POLNS
AccessionPrimary (citable) accession number: B1XSN1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families