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Protein

Methionyl-tRNA formyltransferase

Gene

fmt

Organism
Polynucleobacter necessarius subsp. necessarius (strain STIR1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP.UniRule annotation

Catalytic activityi

10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = tetrahydrofolate + N-formylmethionyl-tRNA(fMet).UniRule annotation

GO - Molecular functioni

  1. methionyl-tRNA formyltransferase activity Source: UniProtKB-HAMAP
  2. methyltransferase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciPNEC452638:GI4T-1773-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionyl-tRNA formyltransferaseUniRule annotation (EC:2.1.2.9UniRule annotation)
Gene namesi
Name:fmtUniRule annotation
Ordered Locus Names:Pnec_1777
OrganismiPolynucleobacter necessarius subsp. necessarius (strain STIR1)
Taxonomic identifieri452638 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter
ProteomesiUP000006582: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Methionyl-tRNA formyltransferasePRO_1000098425Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi452638.Pnec_1777.

Structurei

3D structure databases

ProteinModelPortaliB1XSN1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1274Tetrahydrofolate (THF) bindingUniRule annotation

Sequence similaritiesi

Belongs to the Fmt family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0223.
HOGENOMiHOG000261177.
KOiK00604.
OMAiAESTIDW.
OrthoDBiEOG6B09WV.

Family and domain databases

Gene3Di3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPiMF_00182. Formyl_trans.
InterProiIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
IPR001555. GART_AS.
IPR015518. Met_tRNA_Form_TA-like.
[Graphical view]
PANTHERiPTHR11138. PTHR11138. 1 hit.
PfamiPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
SSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00460. fmt. 1 hit.
PROSITEiPS00373. GART. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1XSN1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIVFAGTPE FAAQAVRAIH AAGHEIVLAF TQPDRRAGRG MHLQASPVKE
60 70 80 90 100
FALQKNIPIL QPETLRRTSA DPQKKAQAEE AYKSLSAIEF DAMVVVAYGL
110 120 130 140 150
ILPQEILDIT EKPGRHGSFN IHASLLPRWR GAAPIQRAIE AGDAKTGVCI
160 170 180 190 200
MQMDAGLDTG DTVLVVELDI ARDETSASLH DRLAGLGADL IVNALDVLQQ
210 220 230 240 250
GKTMIRSPQA IKGITYAEKI LKSEAEIDWT LSAQEIDQRI RAFNPFPGAS
260 270 280 290 300
SKLNGYAIKL WNSRLADSQA FSMVGGAGDV LEFGRDGAYI QCGEGVLEVL
310 320 330
EMQKPGGKKM AAKACIQPIG AGEKLLHFQL KE
Length:332
Mass (Da):35,891
Last modified:May 20, 2008 - v1
Checksum:i5699A912ECC23449
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001010 Genomic DNA. Translation: ACB44830.1.
RefSeqiYP_001798444.1. NC_010531.1.

Genome annotation databases

EnsemblBacteriaiACB44830; ACB44830; Pnec_1777.
GeneIDi6183844.
KEGGipne:Pnec_1777.
PATRICi22972892. VBIPolNec8289_2039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001010 Genomic DNA. Translation: ACB44830.1.
RefSeqiYP_001798444.1. NC_010531.1.

3D structure databases

ProteinModelPortaliB1XSN1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi452638.Pnec_1777.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB44830; ACB44830; Pnec_1777.
GeneIDi6183844.
KEGGipne:Pnec_1777.
PATRICi22972892. VBIPolNec8289_2039.

Phylogenomic databases

eggNOGiCOG0223.
HOGENOMiHOG000261177.
KOiK00604.
OMAiAESTIDW.
OrthoDBiEOG6B09WV.

Enzyme and pathway databases

BioCyciPNEC452638:GI4T-1773-MONOMER.

Family and domain databases

Gene3Di3.10.25.10. 1 hit.
3.40.50.170. 1 hit.
HAMAPiMF_00182. Formyl_trans.
InterProiIPR005794. Fmt.
IPR005793. Formyl_trans_C.
IPR002376. Formyl_transf_N.
IPR011034. Formyl_transferase_C-like.
IPR001555. GART_AS.
IPR015518. Met_tRNA_Form_TA-like.
[Graphical view]
PANTHERiPTHR11138. PTHR11138. 1 hit.
PfamiPF02911. Formyl_trans_C. 1 hit.
PF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMiSSF50486. SSF50486. 1 hit.
SSF53328. SSF53328. 1 hit.
TIGRFAMsiTIGR00460. fmt. 1 hit.
PROSITEiPS00373. GART. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: STIR1.

Entry informationi

Entry nameiFMT_POLNS
AccessioniPrimary (citable) accession number: B1XSN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: January 7, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.