B1XS66 (PTH_POLNS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidyl-tRNA hydrolase
Short name=PTH
EC=3.1.1.29

Gene names

Name:	pth
Ordered Locus Names:	Pnec_1627

Organism

Polynucleobacter necessarius (strain STIR1) [Complete proteome] [HAMAP]

Taxonomic identifier

452638 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Polynucleobacter

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity. HAMAP MF_00083
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA. HAMAP MF_00083
Subunit structure	Monomer By similarity. HAMAP MF_00083
Subcellular location	Cytoplasm By similarity HAMAP MF_00083.
Sequence similarities	Belongs to the PTH family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Peptidyl-tRNA hydrolase HAMAP MF_00083		PRO_1000092967

Sequences

	Sequence		Length	Mass (Da)
	B1XS66 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 3E32B98FF1BDA068	FASTA	196	21,724
	10 20 30 40 50 60 MTKLIVGLGN PGEEHEEDRH NAGFWFVDAL AKQLGVRFES EKRFHGKVAK AKWEGEDLFL 70 80 90 100 110 120 LKPSTYMNLS GQSVGALCRF HKIMPADILV VQDELDIKPG SARLKLGGGT GGHNGLKDIQ 130 140 150 160 170 180 AHLSTPNYWR LRLGIGHPRD IAGDGRPMDV ADYVLRRPQL TEQKLINTSI ENGLNILSLF 190 LKGDTQTAMM ELHSKG

References

[1]

"Complete sequence of Polynucleobacter necessarius STIR1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Hahn M., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: STIR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001010 Genomic DNA. Translation: ACB44701.1.
RefSeq	YP_001798315.1. NC_010531.1.
3D structure databases
ProteinModelPortal	B1XS66.
ModBase	Search...
Protein-protein interaction databases
STRING	B1XS66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6183801.
GenomeReviews	Gene locus Pnec_1627 in contig CP001010_GR.
KEGG	pne:Pnec_1627.
PATRIC	22972546. VBIPolNec8289_1868.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG610927.
OMA	SHAGHNG.
ProtClustDB	PRK05426.
Family and domain databases
HAMAP	MF_00083. Pept_tRNA_hydro_bact. [Tree]
InterPro	IPR001328. Pept_tRNA_hydro. IPR018171. Pept_tRNA_hydro_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.1470. Pept_tRNA_hydro. 1 hit.
KO	K01056.
PANTHER	PTHR17224. Pept_tRNA_hydro. 1 hit.
Pfam	PF01195. Pept_tRNA_hydro. 1 hit. [Graphical view]
SUPFAM	SSF53178. Pept_tRNA_hydro. 1 hit.
TIGRFAMs	TIGR00447. Pth. 1 hit.
PROSITE	PS01195. PEPT_TRNA_HYDROL_1. False negative. PS01196. PEPT_TRNA_HYDROL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTH_POLNS

Accession

Primary (citable) accession number: B1XS66

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program