B1XS63 (ISPE_POLNS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Pnec_1624

Organism

Polynucleobacter necessarius (strain STIR1) [Complete proteome] [HAMAP]

Taxonomic identifier

452638 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Polynucleobacter

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000190696
Regions
	Nucleotide binding	97 – 107	11	ATP Potential
Sites
	Active site	14	1	By similarity
	Active site	139	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1XS63 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: CB6C73ABE2C061D1	FASTA	296	32,519
	10 20 30 40 50 60 MVNLDSLSLR SPAKLNLFLH IVGRRTDGYH LLQSVFQLID WCDTLHLKRI SENVVRRINP 70 80 90 100 110 120 IPGVAPEHDL VVRAANLLKD FCQFEGGVEI NLQKEIPMGA GMGGGSSDAA TTLIGLNALW 130 140 150 160 170 180 SLNLSKETLC ALGLKLGADV PFFIFGKNAF VEGVGEKMRE ISLETPDFLV IFPNRGIATA 190 200 210 220 230 240 SIFQDPELTR DHAQITIDGF LTSPLLYQSN DCQAVAMRIY PEVKQALDWI TQAVPGSQPR 250 260 270 280 290 MSGSGSSVFA VLDSKTDIAK LKNFLQNLPK GWVGRVVRGL NKNPAYNLIS FLQIDL

References

[1]

"Complete sequence of Polynucleobacter necessarius STIR1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Hahn M., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: STIR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001010 Genomic DNA. Translation: ACB44698.1.
RefSeq	YP_001798312.1. NC_010531.1.
3D structure databases
ProteinModelPortal	B1XS63.
ModBase	Search...
Protein-protein interaction databases
STRING	B1XS63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6182966.
GenomeReviews	Gene locus Pnec_1624 in contig CP001010_GR.
KEGG	pne:Pnec_1624.
PATRIC	22972538. VBIPolNec8289_1865.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_POLNS

Accession

Primary (citable) accession number: B1XS63

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program