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Protein

Argininosuccinate synthase

Gene

argG

Organism
Polynucleobacter necessarius subsp. necessarius (strain STIR1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei90 – 901CitrullineUniRule annotation
Binding sitei95 – 951CitrullineUniRule annotation
Binding sitei120 – 1201ATP; via amide nitrogenUniRule annotation
Binding sitei122 – 1221AspartateUniRule annotation
Binding sitei126 – 1261AspartateUniRule annotation
Binding sitei126 – 1261CitrullineUniRule annotation
Binding sitei127 – 1271AspartateUniRule annotation
Binding sitei130 – 1301CitrullineUniRule annotation
Binding sitei182 – 1821CitrullineUniRule annotation
Binding sitei191 – 1911CitrullineUniRule annotation
Binding sitei267 – 2671CitrullineUniRule annotation
Binding sitei279 – 2791CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPUniRule annotation

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPNEC452638:GI4T-1467-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Pnec_1468
OrganismiPolynucleobacter necessarius subsp. necessarius (strain STIR1)
Taxonomic identifieri452638 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaePolynucleobacter
ProteomesiUP000006582 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Argininosuccinate synthasePRO_1000089048Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi452638.Pnec_1468.

Structurei

3D structure databases

ProteinModelPortaliB1XRS6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1XRS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDIKKAVLA YSGGLDTSVI LKWLQDTYGC EIVTFTADFG QGEELEPARA
60 70 80 90 100
KALQFGIKPE NIFIDDLCEE FVRDFVFPMF RANTIYEGEY LLGTSIARPL
110 120 130 140 150
IAKRQIEIAR LTGADSVSHG ATGKGNDQVR FELGYYALEP GIKVIVPWRE
160 170 180 190 200
WDLLSREKLM AYAEKHGIPV EMRHKQGGSP YSMDANLLHI SYEGRHLENP
210 220 230 240 250
NAEAEESMWR WTVSPEKAPD APEIIEIEFK SGDPVAINGK AYKPHELLAE
260 270 280 290 300
LNRIGGMHGI GRLDLVENRF VGMKSRGCYE TPGGTILLKA HRGIESITLD
310 320 330 340 350
REVAHLKDDL MPRYASLIYN GLWWAPERLA LQTLIDHTQQ AVNGIVRLKL
360 370 380 390 400
YKGSVSVISR DSANTLFDQN IATFDDDGGA YNQADAGGFI KLNALRMRIA
410
ETARRKRAKK
Length:410
Mass (Da):45,930
Last modified:May 20, 2008 - v1
Checksum:iF8DEEA5F8C674937
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001010 Genomic DNA. Translation: ACB44561.1.
RefSeqiYP_001798175.1. NC_010531.1.

Genome annotation databases

EnsemblBacteriaiACB44561; ACB44561; Pnec_1468.
KEGGipne:Pnec_1468.
PATRICi22972179. VBIPolNec8289_1688.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001010 Genomic DNA. Translation: ACB44561.1.
RefSeqiYP_001798175.1. NC_010531.1.

3D structure databases

ProteinModelPortaliB1XRS6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi452638.Pnec_1468.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB44561; ACB44561; Pnec_1468.
KEGGipne:Pnec_1468.
PATRICi22972179. VBIPolNec8289_1688.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciPNEC452638:GI4T-1467-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: STIR1.

Entry informationi

Entry nameiASSY_POLNS
AccessioniPrimary (citable) accession number: B1XRS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: April 1, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.