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B1XNE1 (BIOB_SYNP2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:SYNPCC7002_A0309
OrganismSynechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum) [Complete proteome] [HAMAP]
Taxonomic identifier32049 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367Biotin synthase HAMAP-Rule MF_01694
PRO_0000381675

Sites

Metal binding911Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding951Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding981Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1361Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1731Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2331Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding3031Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B1XNE1 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 6B5760293D540FCC

FASTA36739,869
        10         20         30         40         50         60 
MVQVPIKPTP QTPAPTTAIP NGPALEAWLN DWAQRIINGD RLTRTEALRL TAITGPENIL 

        70         80         90        100        110        120 
KLCAAADLIR HECCGNTVDL CSIVNVKSGS CSENCSFCSQ SAHHPGEDSP VYGLKTAEEI 

       130        140        150        160        170        180 
IEQAKAAEAA GAKRFCLVSQ GRGIKYNSPR DTEFEQILET VRRIQAETAI KPCCALGEVT 

       190        200        210        220        230        240 
PEQAQQLREA GVTRYNHNLE ASENFFPDIV TTHSWQDRVE TIKNLKAAGI QACSGGIMGL 

       250        260        270        280        290        300 
GESWEDRVDL AIALRDLEVE SVPLNLLNPR QGTPLGSNDR LDVYEALKCM AIFRFILPEQ 

       310        320        330        340        350        360 
IIRYAGGREA VMGELQHLGL KAGINAMLIG HYLTTMGQPP EQDQAMLKSL GLQGGEAPIP 


GQYSAAQ 

« Hide

References

[1]"Complete sequence of Synechococcus sp. PCC 7002."
Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T., Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J., Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.
Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27264 / PCC 7002 / PR-6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000951 Genomic DNA. Translation: ACA98319.1.
RefSeqYP_001733575.1. NC_010475.1.

3D structure databases

ProteinModelPortalB1XNE1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING32049.SYNPCC7002_A0309.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACA98319; ACA98319; SYNPCC7002_A0309.
GeneID6056538.
KEGGsyp:SYNPCC7002_A0309.
PATRIC23815056. VBISynSp37135_0491.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK892897.

Enzyme and pathway databases

BioCycSSP32049:GKF7-309-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_SYNP2
AccessionPrimary (citable) accession number: B1XNE1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 20, 2008
Last modified: February 19, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways