B1XLQ9 (LEU1_SYNP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-isopropylmalate synthase
EC=2.3.3.13

Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase

Gene names

Name:	leuA
Ordered Locus Names:	SYNPCC7002_A1356

Organism

Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum) [Complete proteome] [HAMAP]

Taxonomic identifier

32049 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechococcus

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) By similarity. HAMAP-Rule MF_01025
Catalytic activity	Acetyl-CoA + 3-methyl-2-oxobutanoate + H2O = (2S)-2-isopropylmalate + CoA. HAMAP-Rule MF_01025
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4. HAMAP-Rule MF_01025
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_01025
Sequence similarities	Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-isopropylmalate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	2-isopropylmalate synthase HAMAP-Rule MF_01025		PRO_1000149313

Sequences

	Sequence		Length	Mass (Da)
	B1XLQ9 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 54309D752D66DC5E	FASTA	533	57,650
	10 20 30 40 50 60 MNNQDDRIII FDTTLRDGEQ SPGATLNGDE KLAIARALAR LGVDVIEAGF PRASRGDFDA 70 80 90 100 110 120 VQRIAAEVGT ENGPTICGLA RATKGDIEAA GNALKPAFSN RIHTFIATSD IHLEYKLRKS 130 140 150 160 170 180 RKDVLAIAPE MVAYAKTFTN DVEFSPEDAG RSDPEFLYQI LEAVIDAGAT TVNIPDTVGY 190 200 210 220 230 240 TTPAEFGALI KGIKENVPNI DRAIISVHGH NDLGLAVANF LEAVKNGARQ LECTINGIGE 250 260 270 280 290 300 RAGNAALEEL VMALHVRRQY YNPFLGRPAD SEAPLTSINT KEIYKTSRLV SNLTGLSVQA 310 320 330 340 350 360 NKAIVGLNAF AHESGIHQDG VLKNKLTYEI MDAQSIGLTD NQIILGKHSG RNAFRTRLAE 370 380 390 400 410 420 LGFDLSDNDL NKAFLRFKDL ADKKKEITDW DLEAIVKDET QQPPELFRLE LVQVSCGDHA 430 440 450 460 470 480 QPTATITIRT PGGKELTDAA IGTGPVDAIY KAINRVVQVP NELIEYSVQS VTAGIDAIGE 490 500 510 520 530 VTIRLRHEGR IYSGHAANTD IVVASARAYI SALNRLYAAL QEDVSANPAK ASL

References

[1]

"Complete sequence of Synechococcus sp. PCC 7002." Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T., Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J., Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27264 / PCC 7002 / PR-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000951 Genomic DNA. Translation: ACA99352.1.
RefSeq	YP_001734608.1. NC_010475.1.
3D structure databases
ProteinModelPortal	B1XLQ9.
ModBase	Search...
Protein-protein interaction databases
STRING	32049.SYNPCC7002_A1356.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACA99352; ACA99352; SYNPCC7002_A1356.
GeneID	6055023.
KEGG	syp:SYNPCC7002_A1356.
PATRIC	23817292. VBISynSp37135_1595.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0119.
HOGENOM	HOG000046859.
KO	K01649.
OMA	VWSVHCH.
ProtClustDB	PRK00915.
Enzyme and pathway databases
BioCyc	SSP32049:GKF7-1357-MONOMER.
UniPathway	UPA00048; UER00070.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01025. LeuA_type1.
InterPro	IPR013709. 2-isopropylmalate_synth_dimer. IPR002034. AIPM/Hcit_synth_CS. IPR013785. Aldolase_TIM. IPR005671. LeuA_bact_synth. IPR000891. PYR_CT. [Graphical view]
Pfam	PF00682. HMGL-like. 1 hit. PF08502. LeuA_dimer. 1 hit. [Graphical view]
SMART	SM00917. LeuA_dimer. 1 hit. [Graphical view]
SUPFAM	SSF110921. 2-isopropylmalate_synth_dimer. 1 hit.
TIGRFAMs	TIGR00973. leuA_bact. 1 hit.
PROSITE	PS00815. AIPM_HOMOCIT_SYNTH_1. 1 hit. PS00816. AIPM_HOMOCIT_SYNTH_2. 1 hit. PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU1_SYNP2

Accession

Primary (citable) accession number: B1XLQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 20, 2008
Last modified:	May 29, 2013
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program