B1XK45 (KATG_SYNP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	SYNPCC7002_A2422

Organism

Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum quadruplicatum) [Complete proteome] [HAMAP]

Taxonomic identifier

32049 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Catalase-peroxidase HAMAP MF_01961		PRO_0000354942
Sites
	Active site	100	1	Proton acceptor By similarity
	Metal binding	268	1	Iron (heme axial ligand) By similarity
	Site	96	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	99 ↔ 227		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-253) By similarity
	Cross-link	227 ↔ 253		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-99) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1XK45 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 37FA7650DB77FF50	FASTA	725	80,606
	10 20 30 40 50 60 MHDHITNTGG KCPVMHGALT TSSMATNMEW WPKALNLDIL HQHDHKTNPM GANFNYQDAV 70 80 90 100 110 120 KTLDVDALKR DLHALMTDSQ DWWPADWGHY GGLMIRMTWH AAGTYRIADG RGGAGTGNQR 130 140 150 160 170 180 FAPINSWPDN TNLDKARRLL WPLKKKYGNK LSWADLIAYA GTIAYESMGL KTFGFAFGRE 190 200 210 220 230 240 DIWHPEKDIY WGSEKEWLAP SDNPHSRYSG ERDLENPLAA VMMGLIYVNP EGVDGNPDPL 250 260 270 280 290 300 KTAHDIRITF SRMAMNDEET VALTAGGHTV GKCHGNGDAT LLGPEPEAAD LDDQGLGWLN 310 320 330 340 350 360 KTQRGIGRNT VTSGIEGAWT TYPTQWDNGY FRLLLNYDWE LKKSPAGAWQ WEPINIKEED 370 380 390 400 410 420 KPVDVEDPSI RLSPIMTDAD MAMKMDPDYR QISERFYQDP AYFAETFARA WFKLTHRDMG 430 440 450 460 470 480 PKSRYIGPDV PQEDLLWQDP IPAGKTDYDP QAVKDKIAAS GLSVSEMVCT AWDSARTFRG 490 500 510 520 530 540 SDKRGGANGA RIRLAPQKDW AGNEPARLAK VLPVLEAIAT ESGASVADVI VLAGNVGIEQ 550 560 570 580 590 600 AAQAAGVEIT VPFAPGRGDA TAEMTDVEGF AVLEPLHDGY RNWLQKDYVV SPEELMLDRT 610 620 630 640 650 660 QLMGLTAPEM TVLVGGMRVL GTNYGGTKHG VLTDREGALT NDFFVNLTDM KYTWKPAGKN 670 680 690 700 710 720 LYEIGDRHTG EVKWTATRVD LVFGCNSILR AYAEVYAQDD SNEKFIQDFV AAWTKVMNAD RFDLA

References

[1]

"Complete sequence of Synechococcus sp. PCC 7002." Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T., Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J., Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A. Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27264 / PCC 7002 / PR-6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000951 Genomic DNA. Translation: ACB00400.1.
RefSeq	YP_001735655.1. NC_010475.1.
3D structure databases
ProteinModelPortal	B1XK45.
SMR	B1XK45. Positions 26-724.
ModBase	Search...
Protein-protein interaction databases
STRING	B1XK45.
Protein family/group databases
PeroxiBase	6257. SspCP01_PCC7002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6057456.
GenomeReviews	Gene locus SYNPCC7002_A2422 in contig CP000951_GR.
KEGG	syp:SYNPCC7002_A2422.
PATRIC	23819486. VBISynSp37135_2673.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	IRLTWHA.
PhylomeDB	B1XK45.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_SYNP2

Accession

Primary (citable) accession number: B1XK45

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program