Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B1XHA3

- RSMF_ECODH

UniProt

B1XHA3 - RSMF_ECODH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation
Binding sitei176 – 1761S-adenosyl-L-methionineUniRule annotation
Binding sitei194 – 1941S-adenosyl-L-methionineUniRule annotation
Active sitei247 – 2471NucleophileUniRule annotation

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. rRNA (cytosine) methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL316385:GJ8B-1919-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
Alternative name(s):
16S rRNA m5C1407 methyltransferaseUniRule annotation
rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
Gene namesi
Name:rsmFUniRule annotation
Ordered Locus Names:ECDH10B_1974
OrganismiEscherichia coli (strain K12 / DH10B)
Taxonomic identifieri316385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001689: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Ribosomal RNA small subunit methyltransferase FPRO_1000147568Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi316385.ECDH10B_1974.

Structurei

3D structure databases

ProteinModelPortaliB1XHA3.
SMRiB1XHA3. Positions 7-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1317S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0144.
HOGENOMiHOG000218115.
KOiK11392.
OMAiAFHALKP.
OrthoDBiEOG6091D0.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F.
InterProiIPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01189. Nol1_Nop2_Fmu. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1XHA3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQHTVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKISV
60 70 80 90 100
ADFLQLTAPY GWTLTPIPWC EEGFWIERDN EDALPLGSTA EHLSGLFYIQ
110 120 130 140 150
EASSMLPVAA LFADGNAPQR VMDVAAAPGS KTTQISARMN NEGAILANEF
160 170 180 190 200
SASRVKVLHA NISRCGISNV ALTHFDGRVF GAAVPEMFDA ILLDAPCSGE
210 220 230 240 250
GVVRKDPDAL KNWSPESNQE IAATQRELID SAFHALRPGG TLVYSTCTLN
260 270 280 290 300
QEENEAVCLW LKETYPDAVE FLPLGDLFPG ANKALTEEGF LHVFPQIYDC
310 320 330 340 350
EGFFVARLRK TQAIPALPAP KYKVGNFPFS PVKDREAGQI RQAATGVGLN
360 370 380 390 400
WDENLRLWQR DKELWLFPVG IEALIGKVRF SRLGIKLAET HNKGYRWQHE
410 420 430 440 450
AVIALASPDN MNAFELTPQE AEEWYRGRDV YPQAAPVADD VLVTFQHQPI
460 470
GLAKRIGSRL KNSYPRELVR DGKLFTGNA
Length:479
Mass (Da):53,228
Last modified:May 20, 2008 - v1
Checksum:i30D2B6AD01FCFF3E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA. Translation: ACB03033.1.
RefSeqiYP_001730811.1. NC_010473.1.

Genome annotation databases

EnsemblBacteriaiACB03033; ACB03033; ECDH10B_1974.
GeneIDi6060501.
KEGGiecd:ECDH10B_1974.
PATRICi18463812. VBIEscCol59506_2001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA. Translation: ACB03033.1 .
RefSeqi YP_001730811.1. NC_010473.1.

3D structure databases

ProteinModelPortali B1XHA3.
SMRi B1XHA3. Positions 7-474.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 316385.ECDH10B_1974.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB03033 ; ACB03033 ; ECDH10B_1974 .
GeneIDi 6060501.
KEGGi ecd:ECDH10B_1974.
PATRICi 18463812. VBIEscCol59506_2001.

Phylogenomic databases

eggNOGi COG0144.
HOGENOMi HOG000218115.
KOi K11392.
OMAi AFHALKP.
OrthoDBi EOG6091D0.

Enzyme and pathway databases

BioCyci ECOL316385:GJ8B-1919-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01579. 16SrRNA_methyltr_F.
InterProi IPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01189. Nol1_Nop2_Fmu. 1 hit.
[Graphical view ]
PRINTSi PR02008. RCMTFAMILY.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00446. nop2p. 1 hit.
PROSITEi PS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse."
    Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R.
    J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / DH10B.

Entry informationi

Entry nameiRSMF_ECODH
AccessioniPrimary (citable) accession number: B1XHA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3