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B1XHA3

- RSMF_ECODH

UniProt

B1XHA3 - RSMF_ECODH

Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation
    Binding sitei176 – 1761S-adenosyl-L-methionineUniRule annotation
    Binding sitei194 – 1941S-adenosyl-L-methionineUniRule annotation
    Active sitei247 – 2471NucleophileUniRule annotation

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. rRNA (cytosine) methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    rRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciECOL316385:GJ8B-1919-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosomal RNA small subunit methyltransferase FUniRule annotation (EC:2.1.1.178UniRule annotation)
    Alternative name(s):
    16S rRNA m5C1407 methyltransferaseUniRule annotation
    rRNA (cytosine-C(5)-)-methyltransferase RsmFUniRule annotation
    Gene namesi
    Name:rsmFUniRule annotation
    Ordered Locus Names:ECDH10B_1974
    OrganismiEscherichia coli (strain K12 / DH10B)
    Taxonomic identifieri316385 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001689: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 479479Ribosomal RNA small subunit methyltransferase FPRO_1000147568Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi316385.ECDH10B_1974.

    Structurei

    3D structure databases

    ProteinModelPortaliB1XHA3.
    SMRiB1XHA3. Positions 7-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1317S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0144.
    HOGENOMiHOG000218115.
    KOiK11392.
    OMAiAFHALKP.
    OrthoDBiEOG6091D0.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01579. 16SrRNA_methyltr_F.
    InterProiIPR001678. Fmu/NOL1/Nop2p.
    IPR018314. Fmu/NOL1/Nop2p_CS.
    IPR011023. Nop2p.
    IPR023267. RCMT.
    IPR023545. rRNA_ssu_MeTfrase_F.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01189. Nol1_Nop2_Fmu. 1 hit.
    [Graphical view]
    PRINTSiPR02008. RCMTFAMILY.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00446. nop2p. 1 hit.
    PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
    PS51686. SAM_MT_RSMB_NOP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1XHA3-1 [UniParc]FASTAAdd to Basket

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    MAQHTVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKISV    50
    ADFLQLTAPY GWTLTPIPWC EEGFWIERDN EDALPLGSTA EHLSGLFYIQ 100
    EASSMLPVAA LFADGNAPQR VMDVAAAPGS KTTQISARMN NEGAILANEF 150
    SASRVKVLHA NISRCGISNV ALTHFDGRVF GAAVPEMFDA ILLDAPCSGE 200
    GVVRKDPDAL KNWSPESNQE IAATQRELID SAFHALRPGG TLVYSTCTLN 250
    QEENEAVCLW LKETYPDAVE FLPLGDLFPG ANKALTEEGF LHVFPQIYDC 300
    EGFFVARLRK TQAIPALPAP KYKVGNFPFS PVKDREAGQI RQAATGVGLN 350
    WDENLRLWQR DKELWLFPVG IEALIGKVRF SRLGIKLAET HNKGYRWQHE 400
    AVIALASPDN MNAFELTPQE AEEWYRGRDV YPQAAPVADD VLVTFQHQPI 450
    GLAKRIGSRL KNSYPRELVR DGKLFTGNA 479
    Length:479
    Mass (Da):53,228
    Last modified:May 20, 2008 - v1
    Checksum:i30D2B6AD01FCFF3E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000948 Genomic DNA. Translation: ACB03033.1.
    RefSeqiYP_001730811.1. NC_010473.1.

    Genome annotation databases

    EnsemblBacteriaiACB03033; ACB03033; ECDH10B_1974.
    GeneIDi6060501.
    KEGGiecd:ECDH10B_1974.
    PATRICi18463812. VBIEscCol59506_2001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000948 Genomic DNA. Translation: ACB03033.1 .
    RefSeqi YP_001730811.1. NC_010473.1.

    3D structure databases

    ProteinModelPortali B1XHA3.
    SMRi B1XHA3. Positions 7-474.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 316385.ECDH10B_1974.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB03033 ; ACB03033 ; ECDH10B_1974 .
    GeneIDi 6060501.
    KEGGi ecd:ECDH10B_1974.
    PATRICi 18463812. VBIEscCol59506_2001.

    Phylogenomic databases

    eggNOGi COG0144.
    HOGENOMi HOG000218115.
    KOi K11392.
    OMAi AFHALKP.
    OrthoDBi EOG6091D0.

    Enzyme and pathway databases

    BioCyci ECOL316385:GJ8B-1919-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01579. 16SrRNA_methyltr_F.
    InterProi IPR001678. Fmu/NOL1/Nop2p.
    IPR018314. Fmu/NOL1/Nop2p_CS.
    IPR011023. Nop2p.
    IPR023267. RCMT.
    IPR023545. rRNA_ssu_MeTfrase_F.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01189. Nol1_Nop2_Fmu. 1 hit.
    [Graphical view ]
    PRINTSi PR02008. RCMTFAMILY.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00446. nop2p. 1 hit.
    PROSITEi PS01153. NOL1_NOP2_SUN. 1 hit.
    PS51686. SAM_MT_RSMB_NOP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse."
      Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R.
      J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / DH10B.

    Entry informationi

    Entry nameiRSMF_ECODH
    AccessioniPrimary (citable) accession number: B1XHA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3