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B1XHA3

- RSMF_ECODH

UniProt

B1XHA3 - RSMF_ECODH

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Protein
Ribosomal RNA small subunit methyltransferase F
Gene
rsmF, ECDH10B_1974
Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491S-adenosyl-L-methionine By similarity
Binding sitei176 – 1761S-adenosyl-L-methionine By similarity
Binding sitei194 – 1941S-adenosyl-L-methionine By similarity
Active sitei247 – 2471Nucleophile By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. rRNA (cytosine) methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciECOL316385:GJ8B-1919-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase F (EC:2.1.1.178)
Alternative name(s):
16S rRNA m5C1407 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmF
Gene namesi
Name:rsmF
Ordered Locus Names:ECDH10B_1974
OrganismiEscherichia coli (strain K12 / DH10B)
Taxonomic identifieri316385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001689: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Ribosomal RNA small subunit methyltransferase FUniRule annotation
PRO_1000147568Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi316385.ECDH10B_1974.

Structurei

3D structure databases

ProteinModelPortaliB1XHA3.
SMRiB1XHA3. Positions 7-474.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1317S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0144.
HOGENOMiHOG000218115.
KOiK11392.
OMAiAFHALKP.
OrthoDBiEOG6091D0.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01579. 16SrRNA_methyltr_F.
InterProiIPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01189. Nol1_Nop2_Fmu. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00446. nop2p. 1 hit.
PROSITEiPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1XHA3-1 [UniParc]FASTAAdd to Basket

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MAQHTVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKISV    50
ADFLQLTAPY GWTLTPIPWC EEGFWIERDN EDALPLGSTA EHLSGLFYIQ 100
EASSMLPVAA LFADGNAPQR VMDVAAAPGS KTTQISARMN NEGAILANEF 150
SASRVKVLHA NISRCGISNV ALTHFDGRVF GAAVPEMFDA ILLDAPCSGE 200
GVVRKDPDAL KNWSPESNQE IAATQRELID SAFHALRPGG TLVYSTCTLN 250
QEENEAVCLW LKETYPDAVE FLPLGDLFPG ANKALTEEGF LHVFPQIYDC 300
EGFFVARLRK TQAIPALPAP KYKVGNFPFS PVKDREAGQI RQAATGVGLN 350
WDENLRLWQR DKELWLFPVG IEALIGKVRF SRLGIKLAET HNKGYRWQHE 400
AVIALASPDN MNAFELTPQE AEEWYRGRDV YPQAAPVADD VLVTFQHQPI 450
GLAKRIGSRL KNSYPRELVR DGKLFTGNA 479
Length:479
Mass (Da):53,228
Last modified:May 20, 2008 - v1
Checksum:i30D2B6AD01FCFF3E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000948 Genomic DNA. Translation: ACB03033.1.
RefSeqiYP_001730811.1. NC_010473.1.

Genome annotation databases

EnsemblBacteriaiACB03033; ACB03033; ECDH10B_1974.
GeneIDi6060501.
KEGGiecd:ECDH10B_1974.
PATRICi18463812. VBIEscCol59506_2001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000948 Genomic DNA. Translation: ACB03033.1 .
RefSeqi YP_001730811.1. NC_010473.1.

3D structure databases

ProteinModelPortali B1XHA3.
SMRi B1XHA3. Positions 7-474.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 316385.ECDH10B_1974.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB03033 ; ACB03033 ; ECDH10B_1974 .
GeneIDi 6060501.
KEGGi ecd:ECDH10B_1974.
PATRICi 18463812. VBIEscCol59506_2001.

Phylogenomic databases

eggNOGi COG0144.
HOGENOMi HOG000218115.
KOi K11392.
OMAi AFHALKP.
OrthoDBi EOG6091D0.

Enzyme and pathway databases

BioCyci ECOL316385:GJ8B-1919-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01579. 16SrRNA_methyltr_F.
InterProi IPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01189. Nol1_Nop2_Fmu. 1 hit.
[Graphical view ]
PRINTSi PR02008. RCMTFAMILY.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00446. nop2p. 1 hit.
PROSITEi PS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse."
    Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R.
    J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / DH10B.

Entry informationi

Entry nameiRSMF_ECODH
AccessioniPrimary (citable) accession number: B1XHA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: June 11, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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