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B1XHA3 (RSMF_ECODH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosomal RNA small subunit methyltransferase F

EC=2.1.1.178
Alternative name(s):
16S rRNA m5C1407 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmF
Gene names
Name:rsmF
Ordered Locus Names:ECDH10B_1974
OrganismEscherichia coli (strain K12 / DH10B) [Complete proteome] [HAMAP]
Taxonomic identifier316385 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA By similarity. HAMAP-Rule MF_01579

Catalytic activity

S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA. HAMAP-Rule MF_01579

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01579.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

rRNA (cytosine) methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Ribosomal RNA small subunit methyltransferase F HAMAP-Rule MF_01579
PRO_1000147568

Regions

Region125 – 1317S-adenosyl-L-methionine binding By similarity

Sites

Active site2471Nucleophile By similarity
Binding site1491S-adenosyl-L-methionine By similarity
Binding site1761S-adenosyl-L-methionine By similarity
Binding site1941S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
B1XHA3 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: 30D2B6AD01FCFF3E

FASTA47953,228
        10         20         30         40         50         60 
MAQHTVYFPD AFLTQMREAM PSTLSFDDFL AACQRPLRRS IRVNTLKISV ADFLQLTAPY 

        70         80         90        100        110        120 
GWTLTPIPWC EEGFWIERDN EDALPLGSTA EHLSGLFYIQ EASSMLPVAA LFADGNAPQR 

       130        140        150        160        170        180 
VMDVAAAPGS KTTQISARMN NEGAILANEF SASRVKVLHA NISRCGISNV ALTHFDGRVF 

       190        200        210        220        230        240 
GAAVPEMFDA ILLDAPCSGE GVVRKDPDAL KNWSPESNQE IAATQRELID SAFHALRPGG 

       250        260        270        280        290        300 
TLVYSTCTLN QEENEAVCLW LKETYPDAVE FLPLGDLFPG ANKALTEEGF LHVFPQIYDC 

       310        320        330        340        350        360 
EGFFVARLRK TQAIPALPAP KYKVGNFPFS PVKDREAGQI RQAATGVGLN WDENLRLWQR 

       370        380        390        400        410        420 
DKELWLFPVG IEALIGKVRF SRLGIKLAET HNKGYRWQHE AVIALASPDN MNAFELTPQE 

       430        440        450        460        470 
AEEWYRGRDV YPQAAPVADD VLVTFQHQPI GLAKRIGSRL KNSYPRELVR DGKLFTGNA 

« Hide

References

[1]"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse."
Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R.
J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / DH10B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000948 Genomic DNA. Translation: ACB03033.1.
RefSeqYP_001730811.1. NC_010473.1.

3D structure databases

ProteinModelPortalB1XHA3.
SMRB1XHA3. Positions 7-474.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING316385.ECDH10B_1974.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB03033; ACB03033; ECDH10B_1974.
GeneID6060501.
KEGGecd:ECDH10B_1974.
PATRIC18463812. VBIEscCol59506_2001.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0144.
HOGENOMHOG000218115.
KOK11392.
OMAAFHALKP.
OrthoDBEOG6091D0.
ProtClustDBPRK11933.

Enzyme and pathway databases

BioCycECOL316385:GJ8B-1919-MONOMER.

Family and domain databases

HAMAPMF_01579. 16SrRNA_methyltr_F.
InterProIPR001678. Fmu/NOL1/Nop2p.
IPR018314. Fmu/NOL1/Nop2p_CS.
IPR011023. Nop2p.
IPR023267. RCMT.
IPR023545. rRNA_ssu_MeTfrase_F.
[Graphical view]
PfamPF01189. Nol1_Nop2_Fmu. 1 hit.
[Graphical view]
PRINTSPR02008. RCMTFAMILY.
TIGRFAMsTIGR00446. nop2p. 1 hit.
PROSITEPS01153. NOL1_NOP2_SUN. 1 hit.
PS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRSMF_ECODH
AccessionPrimary (citable) accession number: B1XHA3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: February 19, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families