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B1XDY5

- PROB_ECODH

UniProt

B1XDY5 - PROB_ECODH

Protein

Glutamate 5-kinase

Gene

proB

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei10 – 101ATPUniRule annotation
    Binding sitei50 – 501SubstrateUniRule annotation
    Binding sitei137 – 1371SubstrateUniRule annotation
    Binding sitei149 – 1491Substrate; via amide nitrogenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi169 – 1702ATPUniRule annotation
    Nucleotide bindingi211 – 2177ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate 5-kinase activity Source: UniProtKB-HAMAP
    3. RNA binding Source: InterPro

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL316385:GJ8B-222-MONOMER.
    UniPathwayiUPA00098; UER00359.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
    Alternative name(s):
    Gamma-glutamyl kinaseUniRule annotation
    Short name:
    GKUniRule annotation
    Gene namesi
    Name:proBUniRule annotation
    Ordered Locus Names:ECDH10B_0224
    OrganismiEscherichia coli (strain K12 / DH10B)
    Taxonomic identifieri316385 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001689: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Glutamate 5-kinasePRO_1000125232Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi316385.ECDH10B_0224.

    Structurei

    3D structure databases

    ProteinModelPortaliB1XDY5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini275 – 35379PUAUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glutamate 5-kinase family.UniRule annotation
    Contains 1 PUA domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0263.
    HOGENOMiHOG000246369.
    KOiK00931.
    OMAiMRMIAGH.
    OrthoDBiEOG6PGK7G.

    Family and domain databases

    Gene3Di2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPiMF_00456. ProB.
    InterProiIPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000729. GK. 1 hit.
    PRINTSiPR00474. GLU5KINASE.
    SMARTiSM00359. PUA. 1 hit.
    [Graphical view]
    SUPFAMiSSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsiTIGR01027. proB. 1 hit.
    PROSITEiPS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1XDY5-1 [UniParc]FASTAAdd to Basket

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    MSDSQTLVVK LGTSVLTGGS RRLNRAHIVE LVRQCAQLHA AGHRIVIVTS    50
    GAIAAGREHL GYPELPATIA SKQLLAAVGQ SRLIQLWEQL FSIYGIHVGQ 100
    MLLTRADMED RERFLNARDT LRALLDNNIV PVINENDAVA TAEIKVGDND 150
    NLSALAAILA GADKLLLLTD QKGLYTADPR SNPQAELIKD VYGIDDALRA 200
    IAGDSVSGLG TGGMSTKLQA ADVACRAGID TIIAAGSKPG VIGDVMEGIS 250
    VGTLFHAQAT PLENRKRWIF GAPPAGEITV DEGATAAILE RGSSLLPKGI 300
    KSVTGNFSRG EVIRICNLEG RDIAHGVSRY NSDALRRIAG HHSQEIDAIL 350
    GYEYGPVAVH RDDMITR 367
    Length:367
    Mass (Da):39,057
    Last modified:May 20, 2008 - v1
    Checksum:i5541040202EBCCE8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000948 Genomic DNA. Translation: ACB01409.1.
    RefSeqiYP_001729187.1. NC_010473.1.

    Genome annotation databases

    EnsemblBacteriaiACB01409; ACB01409; ECDH10B_0224.
    GeneIDi6061924.
    KEGGiecd:ECDH10B_0224.
    PATRICi18460202. VBIEscCol59506_0221.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000948 Genomic DNA. Translation: ACB01409.1 .
    RefSeqi YP_001729187.1. NC_010473.1.

    3D structure databases

    ProteinModelPortali B1XDY5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 316385.ECDH10B_0224.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB01409 ; ACB01409 ; ECDH10B_0224 .
    GeneIDi 6061924.
    KEGGi ecd:ECDH10B_0224.
    PATRICi 18460202. VBIEscCol59506_0221.

    Phylogenomic databases

    eggNOGi COG0263.
    HOGENOMi HOG000246369.
    KOi K00931.
    OMAi MRMIAGH.
    OrthoDBi EOG6PGK7G.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00359 .
    BioCyci ECOL316385:GJ8B-222-MONOMER.

    Family and domain databases

    Gene3Di 2.30.130.10. 1 hit.
    3.40.1160.10. 1 hit.
    HAMAPi MF_00456. ProB.
    InterProi IPR001048. Asp/Glu/Uridylate_kinase.
    IPR001057. Glu/AcGlu_kinase.
    IPR011529. Glu_5kinase.
    IPR005715. Glu_5kinase/COase_Synthase.
    IPR019797. Glutamate_5-kinase_CS.
    IPR002478. PUA.
    IPR015947. PUA-like_domain.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF01472. PUA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000729. GK. 1 hit.
    PRINTSi PR00474. GLU5KINASE.
    SMARTi SM00359. PUA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53633. SSF53633. 1 hit.
    SSF88697. SSF88697. 1 hit.
    TIGRFAMsi TIGR01027. proB. 1 hit.
    PROSITEi PS00902. GLUTAMATE_5_KINASE. 1 hit.
    PS50890. PUA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse."
      Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R.
      J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / DH10B.

    Entry informationi

    Entry nameiPROB_ECODH
    AccessioniPrimary (citable) accession number: B1XDY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3