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Protein

Polyamine aminopropyltransferase

Gene

speE

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.UniRule annotation

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine.UniRule annotation

Pathwayi: spermidine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes spermidine from putrescine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Polyamine aminopropyltransferase (speE)
This subpathway is part of the pathway spermidine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes spermidine from putrescine, the pathway spermidine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9PolyamineUniRule annotation1
Binding sitei33S-adenosylmethioninamineUniRule annotation1
Binding sitei64PolyamineUniRule annotation1
Binding sitei88PolyamineUniRule annotation1
Binding sitei108S-adenosylmethioninamineUniRule annotation1
Active sitei158Proton acceptorUniRule annotation1
Binding sitei165S-adenosylmethioninamine; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Polyamine biosynthesis, Spermidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00248; UER00314.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyamine aminopropyltransferaseUniRule annotation
Alternative name(s):
Putrescine aminopropyltransferaseUniRule annotation
Short name:
PAPTUniRule annotation
Spermidine synthaseUniRule annotation (EC:2.5.1.16UniRule annotation)
Short name:
SPDSUniRule annotation
Short name:
SPDSYUniRule annotation
Gene namesi
Name:speEUniRule annotation
Ordered Locus Names:ECDH10B_0101
OrganismiEscherichia coli (strain K12 / DH10B)
Taxonomic identifieri316385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000992811 – 288Polyamine aminopropyltransferaseAdd BLAST288

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi316385.ECDH10B_0101.

Structurei

3D structure databases

ProteinModelPortaliB1XC96.
SMRiB1XC96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 238PABSUniRule annotationAdd BLAST230

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 54Polyamine bindingUniRule annotation2
Regioni140 – 141S-adenosylmethioninamine bindingUniRule annotation2
Regioni159 – 161Polyamine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the spermidine/spermine synthase family.UniRule annotation
Contains 1 PABS (polyamine biosynthesis) domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCX. Bacteria.
COG0421. LUCA.
HOGENOMiHOG000256146.
KOiK00797.
OMAiSGLWSFT.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1XC96-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKKQWHET LHDQFGQYFA VDNVLYHEKT DHQDLIIFEN AAFGRVMALD
60 70 80 90 100
GVVQTTERDE FIYHEMMTHV PLLAHGHAKH VLIIGGGDGA MLREVTRHKN
110 120 130 140 150
VESITMVEID AGVVSFCRQY LPNHNAGSYD DPRFKLVIDD GVNFVNQTSQ
160 170 180 190 200
TFDVIISDCT DPIGPGESLF TSAFYEGCKR CLNPGGIFVA QNGVCFLQQE
210 220 230 240 250
EAIDSHRKLS HYFSDVGFYQ AAIPTYYGGI MTFAWATDND ALRHLSTEII
260 270 280
QARFLASGLK CRYYNPAIHT AAFALPQYLQ DALASQPS
Length:288
Mass (Da):32,321
Last modified:May 20, 2008 - v1
Checksum:i31AE026FF6199E9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA. Translation: ACB01300.1.
RefSeqiWP_000818411.1. NC_010473.1.

Genome annotation databases

EnsemblBacteriaiACB01300; ACB01300; ECDH10B_0101.
KEGGiecd:ECDH10B_0101.
PATRICi18459948. VBIEscCol59506_0101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA. Translation: ACB01300.1.
RefSeqiWP_000818411.1. NC_010473.1.

3D structure databases

ProteinModelPortaliB1XC96.
SMRiB1XC96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316385.ECDH10B_0101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACB01300; ACB01300; ECDH10B_0101.
KEGGiecd:ECDH10B_0101.
PATRICi18459948. VBIEscCol59506_0101.

Phylogenomic databases

eggNOGiENOG4105CCX. Bacteria.
COG0421. LUCA.
HOGENOMiHOG000256146.
KOiK00797.
OMAiSGLWSFT.

Enzyme and pathway databases

UniPathwayiUPA00248; UER00314.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00198. Spermidine_synth. 1 hit.
InterProiIPR030374. PABS.
IPR030373. PABS_CS.
IPR029063. SAM-dependent_MTases.
IPR001045. Spermi_synthase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00417. speE. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPEE_ECODH
AccessioniPrimary (citable) accession number: B1XC96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: November 2, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.