B1XC96 (SPEE_ECODH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine synthase
EC=2.5.1.16

Alternative name(s):
Putrescine aminopropyltransferase
Short name=PAPT
SPDSY

Gene names

Name:	speE
Ordered Locus Names:	ECDH10B_0101

Organism

Escherichia coli (strain K12 / DH10B) [Complete proteome] [HAMAP]

Taxonomic identifier

316385 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli (strain K12)

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor By similarity. HAMAP-Rule MF_00198
Catalytic activity	S-adenosylmethioninamine + putrescine = S-methyl-5'-thioadenosine + spermidine. HAMAP-Rule MF_00198
Pathway	Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. HAMAP-Rule MF_00198
Subunit structure	Homodimer and homotetramer By similarity.
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Spermidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	spermidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Spermidine synthase HAMAP-Rule MF_00198		PRO_1000099281
Regions
	Region	140 – 141	2	S-adenosylmethioninamine binding By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	33	1	S-adenosylmethioninamine By similarity
	Binding site	63	1	Putrescine By similarity
	Binding site	88	1	S-adenosylmethioninamine By similarity
	Binding site	108	1	S-adenosylmethioninamine By similarity
	Binding site	161	1	Putrescine By similarity
	Binding site	226	1	Putrescine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1XC96 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 31AE026FF6199E9F	FASTA	288	32,321
	10 20 30 40 50 60 MAEKKQWHET LHDQFGQYFA VDNVLYHEKT DHQDLIIFEN AAFGRVMALD GVVQTTERDE 70 80 90 100 110 120 FIYHEMMTHV PLLAHGHAKH VLIIGGGDGA MLREVTRHKN VESITMVEID AGVVSFCRQY 130 140 150 160 170 180 LPNHNAGSYD DPRFKLVIDD GVNFVNQTSQ TFDVIISDCT DPIGPGESLF TSAFYEGCKR 190 200 210 220 230 240 CLNPGGIFVA QNGVCFLQQE EAIDSHRKLS HYFSDVGFYQ AAIPTYYGGI MTFAWATDND 250 260 270 280 ALRHLSTEII QARFLASGLK CRYYNPAIHT AAFALPQYLQ DALASQPS

References

[1]

"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / DH10B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000948 Genomic DNA. Translation: ACB01300.1.
RefSeq	YP_001729078.1. NC_010473.1.
3D structure databases
ProteinModelPortal	B1XC96.
SMR	B1XC96. Positions 4-287.
ModBase	Search...
Protein-protein interaction databases
STRING	316385.ECDH10B_0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB01300; ACB01300; ECDH10B_0101.
GeneID	6061294.
KEGG	ecd:ECDH10B_0101.
PATRIC	18459948. VBIEscCol59506_0101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0421.
HOGENOM	HOG000256146.
KO	K00797.
OMA	ELWYTEK.
ProtClustDB	PRK00811.
Enzyme and pathway databases
BioCyc	ECOL316385:GJ8B-99-MONOMER.
UniPathway	UPA00248; UER00314.
Family and domain databases
HAMAP	MF_00198. Spermidine_synth.
InterPro	IPR001045. Spermidine/spermine_synthase. [Graphical view]
PANTHER	PTHR11558. PTHR11558. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. speE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. 1 hit. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEE_ECODH

Accession

Primary (citable) accession number: B1XC96

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program