B1XC50 (APAH_ECODH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
EC=3.6.1.41

Alternative name(s):
Ap4A hydrolase
Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase
Diadenosine tetraphosphatase

Gene names

Name:	apaH
Ordered Locus Names:	ECDH10B_0050

Organism

Escherichia coli (strain K12 / DH10B) [Complete proteome] [HAMAP]

Taxonomic identifier

316385 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli (strain K12)

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP By similarity. HAMAP-Rule MF_00199
Catalytic activity	P1,P(4)-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP. HAMAP-Rule MF_00199
Sequence similarities	Belongs to the Ap4A hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Bis(5'-nucleosyl)-tetraphosphatase, symmetrical HAMAP-Rule MF_00199		PRO_1000099323

Sequences

	Sequence		Length	Mass (Da)
	B1XC50 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: BD6560C73446C1BB	FASTA	280	31,297
	10 20 30 40 50 60 MATYLIGDVH GCYDELIALL HKVEFTPGKD TLWLTGDLVA RGPGSLDVLR YVKSLGDSVR 70 80 90 100 110 120 LVLGNHDLHL LAVFAGISRN KPKDRLTPLL EAPDADELLN WLRRQPLLQI DEEKKLVMAH 130 140 150 160 170 180 AGITPQWDLQ TAKECARDVE AVLSSDSYPF FLDAMYGDMP NNWSPELRGL GRLRFITNAF 190 200 210 220 230 240 TRMRFCFPNG QLDMYSKESP EEAPAPLKPW FAIPGPVAEE YSIAFGHWAS LEGKGTPEGI 250 260 270 280 YALDTGCCWG GTLTCLRWED KQYFVQPSNR HKDLGEAAAS

References

[1]

"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / DH10B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000948 Genomic DNA. Translation: ACB01254.1.
RefSeq	YP_001729032.1. NC_010473.1.
3D structure databases
ProteinModelPortal	B1XC50.
SMR	B1XC50. Positions 2-268.
ModBase	Search...
Protein-protein interaction databases
STRING	316385.ECDH10B_0050.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB01254; ACB01254; ECDH10B_0050.
GeneID	6060780.
KEGG	ecd:ECDH10B_0050.
PATRIC	18459848. VBIEscCol59506_0051.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000251871.
KO	K01525.
OMA	WLRHQPL.
ProtClustDB	PRK00166.
Enzyme and pathway databases
BioCyc	ECOL316385:GJ8B-50-MONOMER.
Family and domain databases
HAMAP	MF_00199. ApaH.
InterPro	IPR004617. ApaH. IPR004843. Metallo_PEstase_dom. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000903. B5n-ttraPtase_sm. 1 hit.
TIGRFAMs	TIGR00668. apaH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APAH_ECODH

Accession

Primary (citable) accession number: B1XC50

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program