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B1XC18

- B1XC18_ECODH

UniProt

B1XC18 - B1XC18_ECODH

Protein
Submitted name:

Homocysteine-N5-methyltetrahydrofolate transmethylase, B12-dependent

Gene

metH

Organism
Escherichia coli (strain K12 / DH10B)
Status
Unreviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation
    Cobalamin.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi247 – 2471ZincUniRule annotation
    Metal bindingi310 – 3101ZincUniRule annotation
    Metal bindingi311 – 3111ZincUniRule annotation
    Metal bindingi759 – 7591Cobalt (cobalamin axial ligand)UniRule annotation
    Binding sitei946 – 9461S-adenosyl-L-methionine
    Binding sitei1134 – 11341S-adenosyl-L-methionine; via carbonyl oxygen
    Binding sitei1138 – 11381Cobalamin; via carbonyl oxygen

    GO - Molecular functioni

    1. cobalamin binding Source: UniProtKB-KW
    2. methionine synthase activity Source: InterPro
    3. S-adenosylmethionine-homocysteine S-methyltransferase activity Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. pteridine-containing compound metabolic process Source: InterPro

    Keywords - Molecular functioni

    MethyltransferaseImported, Transferase

    Keywords - Ligandi

    CobalaminUniRule annotation, Cobalt, Metal-bindingUniRule annotation, S-adenosyl-L-methionineUniRule annotation, ZincUniRule annotation

    Enzyme and pathway databases

    BioCyciECOL316385:GJ8B-4029-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Homocysteine-N5-methyltetrahydrofolate transmethylase, B12-dependentImported
    Gene namesi
    Name:metHImported
    Ordered Locus Names:ECDH10B_4208Imported
    OrganismiEscherichia coli (strain K12 / DH10B)Imported
    Taxonomic identifieri316385 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001689: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular Source: InterPro

    PTM / Processingi

    Proteomic databases

    PRIDEiB1XC18.

    Interactioni

    Protein-protein interaction databases

    STRINGi316385.ECDH10B_4208.

    Structurei

    3D structure databases

    ProteinModelPortaliB1XC18.
    SMRiB1XC18. Positions 6-639, 651-1227.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni834 – 8352Cobalamin-binding
    Regioni1189 – 11902S-adenosyl-L-methionine binding

    Phylogenomic databases

    eggNOGiCOG1410.
    HOGENOMiHOG000251409.
    KOiK00548.
    OMAiLTEHYAM.
    OrthoDBiEOG6091CH.

    Family and domain databases

    Gene3Di1.10.1240.10. 1 hit.
    3.10.196.10. 1 hit.
    3.20.20.20. 1 hit.
    3.20.20.330. 1 hit.
    3.40.50.280. 1 hit.
    InterProiIPR003759. Cbl-bd_cap.
    IPR006158. Cobalamin-bd.
    IPR011005. Dihydropteroate_synth-like.
    IPR011822. MetH.
    IPR000489. Pterin-binding.
    IPR003726. S_MeTrfase.
    IPR004223. VitB12-dep_Met_synth_activ_dom.
    [Graphical view]
    PfamiPF02310. B12-binding. 1 hit.
    PF02607. B12-binding_2. 1 hit.
    PF02965. Met_synt_B12. 1 hit.
    PF00809. Pterin_bind. 1 hit.
    PF02574. S-methyl_trans. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000381. MetH. 1 hit.
    SMARTiSM01018. B12-binding_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF47644. SSF47644. 1 hit.
    SSF51717. SSF51717. 1 hit.
    SSF52242. SSF52242. 1 hit.
    SSF56507. SSF56507. 1 hit.
    SSF82282. SSF82282. 1 hit.
    TIGRFAMsiTIGR02082. metH. 1 hit.
    PROSITEiPS50974. ADOMET_ACTIVATION. 1 hit.
    PS51332. B12_BINDING. 1 hit.
    PS51337. B12_BINDING_NTER. 1 hit.
    PS50970. HCY. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1XC18-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKVEQLRA QLNERILVLD GGMGTMIQSY RLNEADFRGE RFADWPCDLK     50
    GNNDLLVLSK PEVIAAIHNA YFEAGADIIE TNTFNSTTIA MADYQMESLS 100
    AEINFAAAKL ARACADEWTA RTPEKPRYVA GVLGPTNRTA SISPDVNDPA 150
    FRNITFDGLV AAYRESTKAL VEGGADLILI ETVFDTLNAK AAVFAVKTEF 200
    EALGVELPIM ISGTITDASG RTLSGQTTEA FYNSLRHAEA LTFGLNCALG 250
    PDELRQYVQE LSRIAECYVT AHPNAGLPNA FGEYDLDADT MAKQIREWAQ 300
    AGFLNIVGGC CGTTPQHIAA MSRAVEGLAP RKLPEIPVAC RLSGLEPLNI 350
    GEDSLFVNVG ERTNVTGSAK FKRLIKEEKY SEALDVARQQ VENGAQIIDI 400
    NMDEGMLDAE AAMVRFLNLI AGEPDIARVP IMIDSSKWDV IEKGLKCIQG 450
    KGIVNSISMK EGVDAFIHHA KLLRRYGAAV VVMAFDEQGQ ADTRARKIEI 500
    CRRAYKILTE EVGFPPEDII FDPNIFAVAT GIEEHNNYAQ DFIGACEDIK 550
    RELPHALISG GVSNVSFSFR GNDPVREAIH AVFLYYAIRN GMDMGIVNAG 600
    QLAIYDDLPA ELRDAVEDVI LNRRDDGTER LLELAEKYRG SKTDDTANAQ 650
    QAEWRSWEVN KRLEYSLVKG ITEFIEQDTE EARQQATRPI EVIEGPLMDG 700
    MNVVGDLFGE GKMFLPQVVK SARVMKQAVA YLEPFIEASK EQGKTNGKMV 750
    IATVKGDVHD IGKNIVGVVL QCNNYEIVDL GVMVPAEKIL RTAKEVNADL 800
    IGLSGLITPS LDEMVNVAKE MERQGFTIPL LIGGATTSKA HTAVKIEQNY 850
    SGPTVYVQNA SRTVGVVAAL LSDTQRDDFV ARTRKEYETV RIQHGRKKPR 900
    TPPVTLEAAR DNDFAFDWQA YTPPVAHRLG VQEVEASIET LRNYIDWTPF 950
    FMTWSLAGKY PRILEDEVVG VEAQRLFKDA NDMLDKLSAE KTLNPRGVVG 1000
    LFPANRVGDD IEIYRDETRT HVINVSHHLR QQTEKTGFAN YCLADFVAPK 1050
    LSGKADYIGA FAVTGGLEED ALADAFEAQH DDYNKIMVKA LADRLAEAFA 1100
    EYLHERVRKV YWGYAPNENL SNEELIRENY QGIRPAPGYP ACPEHTEKAT 1150
    IWELLEVEKH TGMKLTESFA MWPGASVSGW YFSHPDSKYY AVAQIQRDQV 1200
    EDYARRKGMS VTEVERWLAP NLGYDAD 1227
    Length:1,227
    Mass (Da):135,997
    Last modified:May 20, 2008 - v1
    Checksum:i91F0CAA1E9127D9A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000948 Genomic DNA. Translation: ACB05018.1.
    RefSeqiYP_001732796.1. NC_010473.1.

    Genome annotation databases

    EnsemblBacteriaiACB05018; ACB05018; ECDH10B_4208.
    GeneIDi6059383.
    KEGGiecd:ECDH10B_4208.
    PATRICi18468421. VBIEscCol59506_4235.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000948 Genomic DNA. Translation: ACB05018.1 .
    RefSeqi YP_001732796.1. NC_010473.1.

    3D structure databases

    ProteinModelPortali B1XC18.
    SMRi B1XC18. Positions 6-639, 651-1227.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 316385.ECDH10B_4208.

    Proteomic databases

    PRIDEi B1XC18.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB05018 ; ACB05018 ; ECDH10B_4208 .
    GeneIDi 6059383.
    KEGGi ecd:ECDH10B_4208.
    PATRICi 18468421. VBIEscCol59506_4235.

    Phylogenomic databases

    eggNOGi COG1410.
    HOGENOMi HOG000251409.
    KOi K00548.
    OMAi LTEHYAM.
    OrthoDBi EOG6091CH.

    Enzyme and pathway databases

    BioCyci ECOL316385:GJ8B-4029-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1240.10. 1 hit.
    3.10.196.10. 1 hit.
    3.20.20.20. 1 hit.
    3.20.20.330. 1 hit.
    3.40.50.280. 1 hit.
    InterProi IPR003759. Cbl-bd_cap.
    IPR006158. Cobalamin-bd.
    IPR011005. Dihydropteroate_synth-like.
    IPR011822. MetH.
    IPR000489. Pterin-binding.
    IPR003726. S_MeTrfase.
    IPR004223. VitB12-dep_Met_synth_activ_dom.
    [Graphical view ]
    Pfami PF02310. B12-binding. 1 hit.
    PF02607. B12-binding_2. 1 hit.
    PF02965. Met_synt_B12. 1 hit.
    PF00809. Pterin_bind. 1 hit.
    PF02574. S-methyl_trans. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000381. MetH. 1 hit.
    SMARTi SM01018. B12-binding_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47644. SSF47644. 1 hit.
    SSF51717. SSF51717. 1 hit.
    SSF52242. SSF52242. 1 hit.
    SSF56507. SSF56507. 1 hit.
    SSF82282. SSF82282. 1 hit.
    TIGRFAMsi TIGR02082. metH. 1 hit.
    PROSITEi PS50974. ADOMET_ACTIVATION. 1 hit.
    PS51332. B12_BINDING. 1 hit.
    PS51337. B12_BINDING_NTER. 1 hit.
    PS50970. HCY. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse."
      Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R.
      J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / DH10BImported.

    Entry informationi

    Entry nameiB1XC18_ECODH
    AccessioniPrimary (citable) accession number: B1XC18
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 20, 2008
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3