B1XC18 (B1XC18_ECODH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Homocysteine-N5-methyltetrahydrofolate transmethylase, B12-dependent EMBL ACB05018.1

Gene names

Name:	metH EMBL ACB05018.1
Ordered Locus Names:	ECDH10B_4208

Organism

Escherichia coli (strain K12 / DH10B) [Complete proteome] [HAMAP] EMBL ACB05018.1

Taxonomic identifier

316385 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli (strain K12)

Protein attributes

Sequence length	1227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor

Binds 1 zinc ion per subunit By similarity. PIRSR PIRSR000381-1 Cobalamin By similarity. PIRSR PIRSR000381-2

Ontologies

Keywords
Ligand	Cobalamin PIRSR PIRSR000381-2 Cobalt Metal-binding PIRSR PIRSR000381-1 S-adenosyl-L-methionine PIRSR PIRSR000381-2 Zinc PIRSR PIRSR000381-1
Molecular function	Methyltransferase EMBL ACB05018.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pteridine-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro methionine synthase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	834 – 835	2	Cobalamin-binding By similarity PIRSR PIRSR000381-2
	Region	1189 – 1190	2	S-adenosyl-L-methionine binding By similarity PIRSR PIRSR000381-2
Sites
	Metal binding	247	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	310	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	311	1	Zinc By similarity PIRSR PIRSR000381-1
	Metal binding	759	1	Cobalt (cobalamin axial ligand) By similarity PIRSR PIRSR000381-1
	Binding site	946	1	S-adenosyl-L-methionine By similarity PIRSR PIRSR000381-2
	Binding site	1134	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity PIRSR PIRSR000381-2
	Binding site	1138	1	Cobalamin; via carbonyl oxygen By similarity PIRSR PIRSR000381-2

Sequences

	Sequence		Length	Mass (Da)
	B1XC18 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 91F0CAA1E9127D9A	FASTA	1,227	135,997
	10 20 30 40 50 60 MSSKVEQLRA QLNERILVLD GGMGTMIQSY RLNEADFRGE RFADWPCDLK GNNDLLVLSK 70 80 90 100 110 120 PEVIAAIHNA YFEAGADIIE TNTFNSTTIA MADYQMESLS AEINFAAAKL ARACADEWTA 130 140 150 160 170 180 RTPEKPRYVA GVLGPTNRTA SISPDVNDPA FRNITFDGLV AAYRESTKAL VEGGADLILI 190 200 210 220 230 240 ETVFDTLNAK AAVFAVKTEF EALGVELPIM ISGTITDASG RTLSGQTTEA FYNSLRHAEA 250 260 270 280 290 300 LTFGLNCALG PDELRQYVQE LSRIAECYVT AHPNAGLPNA FGEYDLDADT MAKQIREWAQ 310 320 330 340 350 360 AGFLNIVGGC CGTTPQHIAA MSRAVEGLAP RKLPEIPVAC RLSGLEPLNI GEDSLFVNVG 370 380 390 400 410 420 ERTNVTGSAK FKRLIKEEKY SEALDVARQQ VENGAQIIDI NMDEGMLDAE AAMVRFLNLI 430 440 450 460 470 480 AGEPDIARVP IMIDSSKWDV IEKGLKCIQG KGIVNSISMK EGVDAFIHHA KLLRRYGAAV 490 500 510 520 530 540 VVMAFDEQGQ ADTRARKIEI CRRAYKILTE EVGFPPEDII FDPNIFAVAT GIEEHNNYAQ 550 560 570 580 590 600 DFIGACEDIK RELPHALISG GVSNVSFSFR GNDPVREAIH AVFLYYAIRN GMDMGIVNAG 610 620 630 640 650 660 QLAIYDDLPA ELRDAVEDVI LNRRDDGTER LLELAEKYRG SKTDDTANAQ QAEWRSWEVN 670 680 690 700 710 720 KRLEYSLVKG ITEFIEQDTE EARQQATRPI EVIEGPLMDG MNVVGDLFGE GKMFLPQVVK 730 740 750 760 770 780 SARVMKQAVA YLEPFIEASK EQGKTNGKMV IATVKGDVHD IGKNIVGVVL QCNNYEIVDL 790 800 810 820 830 840 GVMVPAEKIL RTAKEVNADL IGLSGLITPS LDEMVNVAKE MERQGFTIPL LIGGATTSKA 850 860 870 880 890 900 HTAVKIEQNY SGPTVYVQNA SRTVGVVAAL LSDTQRDDFV ARTRKEYETV RIQHGRKKPR 910 920 930 940 950 960 TPPVTLEAAR DNDFAFDWQA YTPPVAHRLG VQEVEASIET LRNYIDWTPF FMTWSLAGKY 970 980 990 1000 1010 1020 PRILEDEVVG VEAQRLFKDA NDMLDKLSAE KTLNPRGVVG LFPANRVGDD IEIYRDETRT 1030 1040 1050 1060 1070 1080 HVINVSHHLR QQTEKTGFAN YCLADFVAPK LSGKADYIGA FAVTGGLEED ALADAFEAQH 1090 1100 1110 1120 1130 1140 DDYNKIMVKA LADRLAEAFA EYLHERVRKV YWGYAPNENL SNEELIRENY QGIRPAPGYP 1150 1160 1170 1180 1190 1200 ACPEHTEKAT IWELLEVEKH TGMKLTESFA MWPGASVSGW YFSHPDSKYY AVAQIQRDQV 1210 1220 EDYARRKGMS VTEVERWLAP NLGYDAD

References

[1]

"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / DH10B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000948 Genomic DNA. Translation: ACB05018.1.
RefSeq	YP_001732796.1. NC_010473.1.
3D structure databases
ProteinModelPortal	B1XC18.
SMR	B1XC18. Positions 12-639, 651-1227.
ModBase	Search...
Protein-protein interaction databases
STRING	316385.ECDH10B_4208. B1XC18.
Proteomic databases
PRIDE	B1XC18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB05018; ACB05018; ECDH10B_4208.
GeneID	6059383.
KEGG	ecd:ECDH10B_4208.
PATRIC	18468421. VBIEscCol59506_4235.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1410.
HOGENOM	HOG000251409.
KO	K00548.
OMA	VRKEFWG.
ProtClustDB	PRK09490.
Enzyme and pathway databases
BioCyc	ECOL316385:GJ8B-4029-MONOMER.
Family and domain databases
Gene3D	1.10.1240.10. 1 hit. 3.10.196.10. 1 hit. 3.20.20.20. 1 hit. 3.20.20.330. 1 hit. 3.40.50.280. 1 hit.
InterPro	IPR003759. Cbl-bd_cap. IPR006158. Cobalamin-bd. IPR011005. Dihydropteroate_synth-like. IPR011822. MetH. IPR000489. Pterin-binding. IPR003726. S_MeTrfase. IPR004223. VitB12-dep_Met_synth_activ_dom. [Graphical view]
PANTHER	PTHR21091:SF9. PTHR21091:SF9. 1 hit.
Pfam	PF02310. B12-binding. 1 hit. PF02607. B12-binding_2. 1 hit. PF02965. Met_synt_B12. 1 hit. PF00809. Pterin_bind. 1 hit. PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF000381. MetH. 1 hit.
SMART	SM01018. B12-binding_2. 1 hit. [Graphical view]
SUPFAM	SSF52242. Cbl-bd. 1 hit. SSF51717. DHP_synth_like. 1 hit. SSF56507. Met_synth_B12. 1 hit. SSF47644. Met_synth_Cbl-bd. 1 hit. SSF82282. S_methyl_trans. 1 hit.
TIGRFAMs	TIGR02082. metH. 1 hit.
PROSITE	PS50974. ADOMET_ACTIVATION. 1 hit. PS51332. B12_BINDING. 1 hit. PS51337. B12_BINDING_NTER. 1 hit. PS50970. HCY. 1 hit. PS50972. PTERIN_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1XC18_ECODH

Accession

Primary (citable) accession number: B1XC18

Entry history

Integrated into UniProtKB/TrEMBL:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)