B1XC12 (META_ECODH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine O-succinyltransferase
EC=2.3.1.46

Alternative name(s):
Homoserine O-transsuccinylase
Short name=HTS

Gene names

Name:	metA
Ordered Locus Names:	ECDH10B_4202

Organism

Escherichia coli (strain K12 / DH10B) [Complete proteome] [HAMAP]

Taxonomic identifier

316385 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli (strain K12)

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinyl-CoA + L-homoserine = CoA + O-succinyl-L-homoserine. HAMAP-Rule MF_00295
Enzyme regulation	Allosterically inhibited by SAM and methionine By similarity. HAMAP-Rule MF_00295
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1. HAMAP-Rule MF_00295
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HTS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-methionine biosynthetic process from homoserine via O-succinyl-L-homoserine and cystathionine Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	homoserine O-succinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Homoserine O-succinyltransferase HAMAP-Rule MF_00295		PRO_1000115179
Sites
	Active site	142	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	B1XC12 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: A2C395A9A9CFE2C0	FASTA	309	35,727
	10 20 30 40 50 60 MPIRVPDELP AVNFLREENV FVMTTSRASG QEIRPLKVLI LNLMPKKIET ENQFLRLLSN 70 80 90 100 110 120 SPLQVDIQLL RIDSRESRNT PAEHLNNFYC NFEDIQDQNF DGLIVTGAPL GLVEFNDVAY 130 140 150 160 170 180 WPQIKQVLEW SKDHVTSTLF VCWAVQAALN ILYGIPKQTR TEKLSGVYEH HILHPHALLT 190 200 210 220 230 240 RGFDDSFLAP HSRYADFPAA LIRDYTDLEI LAETEEGDAY LFASKDKRIA FVTGHPEYDA 250 260 270 280 290 300 QTLAQEFFRD VEAGLDPDVP YNYFPHNDPQ NTPRASWRSH GNLLFTNWLN YYVYQITPYD LRHMNPTLD

References

[1]

"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / DH10B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000948 Genomic DNA. Translation: ACB05012.1.
RefSeq	YP_001732790.1. NC_010473.1.
3D structure databases
ProteinModelPortal	B1XC12.
SMR	B1XC12. Positions 2-297.
ModBase	Search...
Protein-protein interaction databases
STRING	316385.ECDH10B_4202.
Proteomic databases
PRIDE	B1XC12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB05012; ACB05012; ECDH10B_4202.
GeneID	6059662.
KEGG	ecd:ECDH10B_4202.
PATRIC	18468407. VBIEscCol59506_4228.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1897.
HOGENOM	HOG000115049.
KO	K00651.
OMA	WRSHRNL.
ProtClustDB	PRK05368.
Enzyme and pathway databases
BioCyc	ECOL316385:GJ8B-4023-MONOMER.
UniPathway	UPA00051; UER00075.
Family and domain databases
HAMAP	MF_00295. Homoser_O-succinyltr.
InterPro	IPR005697. Homserine_suc_trans. [Graphical view]
PANTHER	PTHR20919. PTHR20919. 1 hit.
Pfam	PF04204. HTS. 1 hit. [Graphical view]
PIRSF	PIRSF000450. H_ser_succinyltr. 1 hit.
ProDom	PD037892. Homserine_suc_trans. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01001. metA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

META_ECODH

Accession

Primary (citable) accession number: B1XC12

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program