Reviewed, UniProtKB/Swiss-Prot B1XBX8 (MURI_ECODH)

Names and origin

Protein names

Recommended name:
    Glutamate racemase
    EC=5.1.1.3

Gene names

Name:	murI
Ordered Locus Names:	ECDH10B_4156

Organism

Escherichia coli (strain K12 / DH10B) [Complete proteome] [HAMAP]

Taxonomic identifier

316385 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the (R)-glutamate required for cell wall biosynthesis By similarity. HAMAP MF_00258
Catalytic activity	L-glutamate = D-glutamate. HAMAP MF_00258
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00258
Sequence similarities	Belongs to the aspartate/glutamate racemases family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutamate racemase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Glutamate racemase HAMAP MF_00258		PRO_1000114041

Sequences

	Sequence		Length	Mass (Da)
	B1XBX8-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: EC87281B577B8E93	FASTA	285	31,002
	10 20 30 40 50 60 MATKLQDGNT PCLAATPSEP RPTVLVFDSG VGGLSVYDEI RHLLPDLHYI YAFDNVAFPY 70 80 90 100 110 120 GEKSEAFIVE RVVAIVTAVQ ERYPLALAVV ACNTASTVSL PALREKFDFP VVGVVPAIKP 130 140 150 160 170 180 AARLTANGIV GLLATRGTVK RSYTHELIAR FANECQIEML GSAEMVELAE AKLHGEDVSL 190 200 210 220 230 240 DALKRILRPW LRMKEPPDTV VLGCTHFPLL QEELLQVLPE GTRLVDSGAA IARRTAWLLE 250 260 270 280 HEAPDAKSAD ANIAFCMAMT PGAEQLLPVL QRYGFETLEK LAVLG

References

[1]

"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008) [PubMed: 18245285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000948 Genomic DNA. Translation: ACB04978.1.
RefSeq	YP_001732756.1.
3D structure databases
SMR	B1XBX8. Positions 20-285.
ModBase	Search...
Genome annotation databases
GeneID	6058260.
GenomeReviews	Gene locus ECDH10B_4156 in contig CP000948_GR.
KEGG	ecd:ECDH10B_4156.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG645102.
OMA	VITQPCP.
Family and domain databases
HAMAP	MF_00258. Glu_racemase. [Tree]
InterPro	IPR015942. Asp/Glu/hydantoin_racemase. IPR001920. Asp/Glu_race. IPR018187. Asp/Glu_racemase_AS. IPR004391. Glu_race. [Graphical view]
Gene3D	G3DSA:3.40.50.1860. Asp/Glu_race. 1 hit.
Pfam	PF01177. Asp_Glu_race. 1 hit. [Graphical view]
TIGRFAMs	TIGR00067. glut_race. 1 hit.
PROSITE	PS00923. ASP_GLU_RACEMASE_1. 1 hit. PS00924. ASP_GLU_RACEMASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURI_ECODH

Accession

Primary (citable) accession number: B1XBX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 20, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)