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B1XB85

- TPIS_ECODH

UniProt

B1XB85 - TPIS_ECODH

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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91SubstrateUniRule annotation
Binding sitei11 – 111SubstrateUniRule annotation
Active sitei95 – 951ElectrophileUniRule annotation
Active sitei167 – 1671Proton acceptorUniRule annotation

GO - Molecular functioni

  1. triose-phosphate isomerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
  2. glycolytic process Source: UniProtKB-HAMAP
  3. pentose-phosphate shunt Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

BioCyciECOL316385:GJ8B-3929-MONOMER.
UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomeraseUniRule annotation (EC:5.3.1.1UniRule annotation)
Short name:
TIMUniRule annotation
Alternative name(s):
Triose-phosphate isomeraseUniRule annotation
Gene namesi
Name:tpiAUniRule annotation
Ordered Locus Names:ECDH10B_4108
OrganismiEscherichia coli (strain K12 / DH10B)
Taxonomic identifieri316385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001689: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255Triosephosphate isomerasePRO_1000096495Add
BLAST

Proteomic databases

PRIDEiB1XB85.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi316385.ECDH10B_4108.

Structurei

Secondary structure

1
255
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Helixi16 – 3015Combined sources
Beta strandi35 – 417Combined sources
Helixi44 – 463Combined sources
Helixi47 – 548Combined sources
Beta strandi57 – 648Combined sources
Beta strandi70 – 734Combined sources
Helixi80 – 867Combined sources
Beta strandi88 – 947Combined sources
Helixi96 – 1016Combined sources
Helixi106 – 11813Combined sources
Beta strandi122 – 1276Combined sources
Helixi131 – 1355Combined sources
Helixi139 – 15416Combined sources
Helixi156 – 1594Combined sources
Beta strandi163 – 1664Combined sources
Helixi169 – 1713Combined sources
Beta strandi172 – 1754Combined sources
Helixi180 – 19516Combined sources
Helixi199 – 2046Combined sources
Beta strandi206 – 2094Combined sources
Turni215 – 2173Combined sources
Helixi218 – 2225Combined sources
Beta strandi229 – 2335Combined sources
Helixi234 – 2374Combined sources
Helixi239 – 25315Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4IOTX-ray1.85A/B1-255[»]
ProteinModelPortaliB1XB85.
SMRiB1XB85. Positions 1-255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0149.
HOGENOMiHOG000226413.
KOiK01803.
OMAiPAIYLDQ.
OrthoDBiEOG66QM23.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_B. TIM_B.
InterProiIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1XB85-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRHPLVMGNW KLNGSRHMVH ELVSNLRKEL AGVAGCAVAI APPEMYIDMA
60 70 80 90 100
KREAEGSHIM LGAQNVDLNL SGAFTGETSA AMLKDIGAQY IIIGHSERRT
110 120 130 140 150
YHKESDELIA KKFAVLKEQG LTPVLCIGET EAENEAGKTE EVCARQIDAV
160 170 180 190 200
LKTQGAAAFE GAVIAYEPVW AIGTGKSATP AQAQAVHKFI RDHIAKVDAN
210 220 230 240 250
IAEQVIIQYG GSVNASNAAE LFAQPDIDGA LVGGASLKAD AFAVIVKAAE

AAKQA
Length:255
Mass (Da):26,972
Last modified:May 20, 2008 - v1
Checksum:iE398251020012D94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA. Translation: ACB04931.1.
RefSeqiYP_001732709.1. NC_010473.1.

Genome annotation databases

EnsemblBacteriaiACB04931; ACB04931; ECDH10B_4108.
GeneIDi6061229.
KEGGiecd:ECDH10B_4108.
PATRICi18468207. VBIEscCol59506_4140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA. Translation: ACB04931.1 .
RefSeqi YP_001732709.1. NC_010473.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4IOT X-ray 1.85 A/B 1-255 [» ]
ProteinModelPortali B1XB85.
SMRi B1XB85. Positions 1-255.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 316385.ECDH10B_4108.

Proteomic databases

PRIDEi B1XB85.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB04931 ; ACB04931 ; ECDH10B_4108 .
GeneIDi 6061229.
KEGGi ecd:ECDH10B_4108.
PATRICi 18468207. VBIEscCol59506_4140.

Phylogenomic databases

eggNOGi COG0149.
HOGENOMi HOG000226413.
KOi K01803.
OMAi PAIYLDQ.
OrthoDBi EOG66QM23.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00189 .
UPA00138 .
BioCyci ECOL316385:GJ8B-3929-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00147_B. TIM_B.
InterProi IPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view ]
PANTHERi PTHR21139. PTHR21139. 1 hit.
Pfami PF00121. TIM. 1 hit.
[Graphical view ]
SUPFAMi SSF51351. SSF51351. 1 hit.
TIGRFAMsi TIGR00419. tim. 1 hit.
PROSITEi PS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse."
    Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R.
    J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / DH10B.

Entry informationi

Entry nameiTPIS_ECODH
AccessioniPrimary (citable) accession number: B1XB85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 20, 2008
Last modified: October 29, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3