B1XAT0 (B1XAT0_ECODH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Thymidine kinase HAMAP-Rule MF_00124 RuleBase RU000544
EC=2.7.1.21 HAMAP-Rule MF_00124 RuleBase RU000544

Gene names

Name:	tdk HAMAP-Rule MF_00124
Ordered Locus Names:	ECDH10B_1298

Organism

Escherichia coli (strain K12 / DH10B) [Complete proteome] [HAMAP] EMBL ACB02409.1

Taxonomic identifier

316385 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli (strain K12)

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124 RuleBase RU000544
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_00124 SAAS SAAS001267
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00124 SAAS SAAS001267.
Sequence similarities	Belongs to the thymidine kinase family. HAMAP-Rule MF_00124 RuleBase RU004165

Ontologies

Keywords
Biological process	DNA synthesis HAMAP-Rule MF_00124 RuleBase RU000544 SAAS SAAS020634
Cellular component	Cytoplasm HAMAP-Rule MF_00124 SAAS SAAS001267
Ligand	ATP-binding HAMAP-Rule MF_00124 RuleBase RU000544 SAAS SAAS020634 Metal-binding HAMAP-Rule MF_00124 SAAS SAAS001267 Nucleotide-binding Zinc HAMAP-Rule MF_00124 SAAS SAAS001267
Molecular function	Kinase HAMAP-Rule MF_00124 RuleBase RU000544 SAAS SAAS020634 EMBL ACB02409.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity HAMAP-Rule MF_00124
	Nucleotide binding	87 – 90	4	ATP By similarity HAMAP-Rule MF_00124
Sites
	Active site	88	1	Proton acceptor By similarity HAMAP-Rule MF_00124
	Metal binding	145	1	Zinc By similarity HAMAP-Rule MF_00124
	Metal binding	147	1	Zinc By similarity HAMAP-Rule MF_00124
	Metal binding	182	1	Zinc By similarity HAMAP-Rule MF_00124
	Metal binding	185	1	Zinc By similarity HAMAP-Rule MF_00124

Sequences

	Sequence		Length	Mass (Da)
	B1XAT0 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: 533166D9EAD20E16	FASTA	205	23,457
	10 20 30 40 50 60 MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS SRIGLSSPAK 70 80 90 100 110 120 LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL SEVVDQLDIP VLCYGLRTDF 130 140 150 160 170 180 RGELFIGSQY LLAWSDKLVE LKTICFCGRK ASMVLRLDQA GRPYNEGEQV VIGGNERYVS 190 200 VCRKHYKEAL QVDSLTAIQE RHRHD

References

[1]

"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / DH10B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000948 Genomic DNA. Translation: ACB02409.1.
RefSeq	YP_001730187.1. NC_010473.1.
3D structure databases
ProteinModelPortal	B1XAT0.
SMR	B1XAT0. Positions 6-187.
ModBase	Search...
Protein-protein interaction databases
STRING	316385.ECDH10B_1298.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB02409; ACB02409; ECDH10B_1298.
GeneID	6058419.
KEGG	ecd:ECDH10B_1298.
PATRIC	18462444. VBIEscCol59506_1319.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076391.
KO	K00857.
OMA	FRYGAMN.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	ECOL316385:GJ8B-1276-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B1XAT0_ECODH

Accession

Primary (citable) accession number: B1XAT0

Entry history

Integrated into UniProtKB/TrEMBL:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)