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Protein

ATP-dependent RNA helicase RhlB

Gene

rhlB

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DEAD-box RNA helicase involved in RNA degradation. Has RNA-dependent ATPase activity and unwinds double-stranded RNA.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 60ATPUniRule annotation8

GO - Molecular functioni

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase RhlBUniRule annotation (EC:3.6.4.13UniRule annotation)
Gene namesi
Name:rhlBUniRule annotation
Ordered Locus Names:ECDH10B_3969
OrganismiEscherichia coli (strain K12 / DH10B)
Taxonomic identifieri316385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

B1X9Z4:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001312901 – 421ATP-dependent RNA helicase RhlBAdd BLAST421

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1X9Z4.
SMRiB1X9Z4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 219Helicase ATP-bindingUniRule annotationAdd BLAST180
Domaini245 – 390Helicase C-terminalUniRule annotationAdd BLAST146

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 37Q motifAdd BLAST29
Motifi165 – 168DEAD box4

Sequence similaritiesi

Belongs to the DEAD box helicase family. RhlB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1J. Bacteria.
COG0513. LUCA.
HOGENOMiHOG000268807.
KOiK03732.
OMAiDEMPVNA.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
HAMAPiMF_00661. DEAD_helicase_RhlB. 1 hit.
InterProiView protein in InterPro
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR023554. RNA_helicase_ATP-dep_RhlB.
IPR014014. RNA_helicase_DEAD_Q_motif.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.

Sequencei

Sequence statusi: Complete.

B1X9Z4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKTHLTEQK FSDFALHPKV VEALEKKGFH NCTPIQALAL PLTLAGRDVA
60 70 80 90 100
GQAQTGTGKT MAFLTSTFHY LLSHPAIADR KVNQPRALIM APTRELAVQI
110 120 130 140 150
HADAEPLAEA TGLKLGLAYG GDGYDKQLKV LESGVDILIG TTGRLIDYAK
160 170 180 190 200
QNHINLGAIQ VVVLDEADRM YDLGFIKDIR WLFRRMPPAN QRLNMLFSAT
210 220 230 240 250
LSYRVRELAF EQMNNAEYIE VEPEQKTGHR IKEELFYPSN EEKMRLLQTL
260 270 280 290 300
IEEEWPDRAI IFANTKHRCE EIWGHLAADG HRVGLLTGDV AQKKRLRILD
310 320 330 340 350
EFTRGDLDIL VATDVAARGL HIPAVTHVFN YDLPDDCEDY VHRIGRTGRA
360 370 380 390 400
GASGHSISLA CEEYALNLPA IETYIGHSIP VSKYNPDALM TDLPKPLRLT
410 420
RPRTGNGPRR TGAPRNRRRS G
Length:421
Mass (Da):47,126
Last modified:May 20, 2008 - v1
Checksum:i48E1ADD025CBFA1A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA. Translation: ACB04809.1.
RefSeqiWP_000047499.1. NC_010473.1.

Genome annotation databases

EnsemblBacteriaiACB04809; ACB04809; ECDH10B_3969.
KEGGiecd:ECDH10B_3969.

Similar proteinsi

Entry informationi

Entry nameiRHLB_ECODH
AccessioniPrimary (citable) accession number: B1X9Z4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 20, 2008
Last modified: October 25, 2017
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families