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B1X8W0

- RHMD_ECODH

UniProt

B1X8W0 - RHMD_ECODH

Protein

L-rhamnonate dehydratase

Gene

rhmD

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the dehydration of L-rhamnonate to 2-keto-3-deoxy-L-rhamnonate (KDR).UniRule annotation

    Catalytic activityi

    L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H2O.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331SubstrateUniRule annotation
    Binding sitei59 – 591SubstrateUniRule annotation
    Metal bindingi226 – 2261MagnesiumUniRule annotation
    Metal bindingi252 – 2521MagnesiumUniRule annotation
    Metal bindingi280 – 2801MagnesiumUniRule annotation
    Sitei302 – 3021Increases basicity of active site HisUniRule annotation
    Active sitei329 – 3291Proton acceptorUniRule annotation
    Binding sitei349 – 3491SubstrateUniRule annotation
    Sitei349 – 3491Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. L-rhamnonate dehydratase activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular amino acid catabolic process Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciECOL316385:GJ8B-2314-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-rhamnonate dehydrataseUniRule annotation (EC:4.2.1.90UniRule annotation)
    Short name:
    RhamDUniRule annotation
    Gene namesi
    Name:rhmDUniRule annotation
    Ordered Locus Names:ECDH10B_2407
    OrganismiEscherichia coli (strain K12 / DH10B)
    Taxonomic identifieri316385 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000001689: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 405405L-rhamnonate dehydratasePRO_0000351692Add
    BLAST

    Proteomic databases

    PRIDEiB1X8W0.

    Interactioni

    Subunit structurei

    Homooctamer; tetramer of dimers.UniRule annotation

    Protein-protein interaction databases

    STRINGi316385.ECDH10B_2407.

    Structurei

    3D structure databases

    ProteinModelPortaliB1X8W0.
    SMRiB1X8W0. Positions 1-405.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG4948.
    HOGENOMiHOG000113755.
    KOiK12661.
    OMAiFCVELEA.
    OrthoDBiEOG68Q0M0.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPiMF_01288. Rhamnon_dehydrat.
    InterProiIPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR023444. L-Rhamnon_dehydrat.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    [Graphical view]
    PANTHERiPTHR13794. PTHR13794. 1 hit.
    PfamiPF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view]
    SMARTiSM00922. MR_MLE. 1 hit.
    [Graphical view]
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    PROSITEiPS00908. MR_MLE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B1X8W0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENIMTLPKI KQVRAWFTGG ATAEKGAGGG DYHDQGANHW IDDHIATPMS    50
    KYRDYEQSRQ SFGINVLGTL VVEVEAENGQ TGFAVSTAGE MGCFIVEKHL 100
    NRFIEGKCVS DIKLIHDQML SATLYYSGSG GLVMNTISCV DLALWDLFGK 150
    VVGLPVYKLL GGAVRDEIQF YATGARPDLA KEMGFIGGKM PTHWGPHDGD 200
    AGIRKDAAMV ADMREKCGED FWLMLDCWMS QDVNYATKLA HACAPYNLKW 250
    IEECLPPQQY ESYRELKRNA PVGMMVTSGE HHGTLQSFRT LSETGIDIMQ 300
    PDVGWCGGLT TLVEIAAIAK SRGQLVVPHG SSVYSHHAVI TFTNTPFSEF 350
    LMTSPDCSTM RPQFDPILLN EPVPVNGRIH KSVLDKPGFG VELNRDCNLK 400
    RPYSH 405
    Length:405
    Mass (Da):44,714
    Last modified:May 20, 2008 - v1
    Checksum:i747D32E809A7B4DC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000948 Genomic DNA. Translation: ACB03407.1.
    RefSeqiYP_001731185.1. NC_010473.1.

    Genome annotation databases

    EnsemblBacteriaiACB03407; ACB03407; ECDH10B_2407.
    GeneIDi6058390.
    KEGGiecd:ECDH10B_2407.
    PATRICi18464683. VBIEscCol59506_2428.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000948 Genomic DNA. Translation: ACB03407.1 .
    RefSeqi YP_001731185.1. NC_010473.1.

    3D structure databases

    ProteinModelPortali B1X8W0.
    SMRi B1X8W0. Positions 1-405.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 316385.ECDH10B_2407.

    Proteomic databases

    PRIDEi B1X8W0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACB03407 ; ACB03407 ; ECDH10B_2407 .
    GeneIDi 6058390.
    KEGGi ecd:ECDH10B_2407.
    PATRICi 18464683. VBIEscCol59506_2428.

    Phylogenomic databases

    eggNOGi COG4948.
    HOGENOMi HOG000113755.
    KOi K12661.
    OMAi FCVELEA.
    OrthoDBi EOG68Q0M0.

    Enzyme and pathway databases

    BioCyci ECOL316385:GJ8B-2314-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPi MF_01288. Rhamnon_dehydrat.
    InterProi IPR029065. Enolase_C-like.
    IPR029017. Enolase_N_like.
    IPR023444. L-Rhamnon_dehydrat.
    IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
    IPR013342. Mandelate_racemase_C.
    IPR013341. Mandelate_racemase_N.
    IPR001354. MR_MLE.
    [Graphical view ]
    PANTHERi PTHR13794. PTHR13794. 1 hit.
    Pfami PF01188. MR_MLE. 1 hit.
    PF02746. MR_MLE_N. 1 hit.
    [Graphical view ]
    SMARTi SM00922. MR_MLE. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    PROSITEi PS00908. MR_MLE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse."
      Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R.
      J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / DH10B.

    Entry informationi

    Entry nameiRHMD_ECODH
    AccessioniPrimary (citable) accession number: B1X8W0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 14, 2008
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Reaction proceeds via a syn dehydration.UniRule annotation

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3