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Protein

L-rhamnonate dehydratase

Gene

rhmD

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the dehydration of L-rhamnonate to 2-keto-3-deoxy-L-rhamnonate (KDR).UniRule annotation

Miscellaneous

Reaction proceeds via a syn dehydration.UniRule annotation

Catalytic activityi

L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33SubstrateUniRule annotation1
Binding sitei59SubstrateUniRule annotation1
Metal bindingi226MagnesiumUniRule annotation1
Metal bindingi252MagnesiumUniRule annotation1
Metal bindingi280MagnesiumUniRule annotation1
Sitei302Increases basicity of active site HisUniRule annotation1
Active sitei329Proton acceptorUniRule annotation1
Binding sitei349SubstrateUniRule annotation1
Sitei349Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
L-rhamnonate dehydrataseUniRule annotation (EC:4.2.1.90UniRule annotation)
Short name:
RhamDUniRule annotation
Gene namesi
Name:rhmDUniRule annotation
Ordered Locus Names:ECDH10B_2407
OrganismiEscherichia coli (strain K12 / DH10B)
Taxonomic identifieri316385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003516921 – 405L-rhamnonate dehydrataseAdd BLAST405

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB1X8W0
SMRiB1X8W0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CXK Bacteria
COG4948 LUCA
HOGENOMiHOG000113755
KOiK12661
OMAiNINWWEE

Family and domain databases

Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_01288 Rhamnon_dehydrat, 1 hit
InterProiView protein in InterPro
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR029065 Enolase_C-like
IPR023444 L-Rhamnon_dehydrat
IPR018110 Mandel_Rmase/mucon_lact_enz_CS
IPR034593 Mandelate_racemase-like
IPR013342 Mandelate_racemase_C
IPR013341 Mandelate_racemase_N_dom
PANTHERiPTHR13794:SF59 PTHR13794:SF59, 1 hit
PfamiView protein in Pfam
PF13378 MR_MLE_C, 1 hit
PF02746 MR_MLE_N, 1 hit
SFLDiSFLDF00006 rhamnonate_dehydratase, 1 hit
SFLDG00179 mandelate_racemase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM00922 MR_MLE, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
PROSITEiView protein in PROSITE
PS00908 MR_MLE_1, 1 hit

Sequencei

Sequence statusi: Complete.

B1X8W0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENIMTLPKI KQVRAWFTGG ATAEKGAGGG DYHDQGANHW IDDHIATPMS
60 70 80 90 100
KYRDYEQSRQ SFGINVLGTL VVEVEAENGQ TGFAVSTAGE MGCFIVEKHL
110 120 130 140 150
NRFIEGKCVS DIKLIHDQML SATLYYSGSG GLVMNTISCV DLALWDLFGK
160 170 180 190 200
VVGLPVYKLL GGAVRDEIQF YATGARPDLA KEMGFIGGKM PTHWGPHDGD
210 220 230 240 250
AGIRKDAAMV ADMREKCGED FWLMLDCWMS QDVNYATKLA HACAPYNLKW
260 270 280 290 300
IEECLPPQQY ESYRELKRNA PVGMMVTSGE HHGTLQSFRT LSETGIDIMQ
310 320 330 340 350
PDVGWCGGLT TLVEIAAIAK SRGQLVVPHG SSVYSHHAVI TFTNTPFSEF
360 370 380 390 400
LMTSPDCSTM RPQFDPILLN EPVPVNGRIH KSVLDKPGFG VELNRDCNLK

RPYSH
Length:405
Mass (Da):44,714
Last modified:May 20, 2008 - v1
Checksum:i747D32E809A7B4DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA Translation: ACB03407.1

Genome annotation databases

EnsemblBacteriaiACB03407; ACB03407; ECDH10B_2407
KEGGiecd:ECDH10B_2407

Similar proteinsi

Entry informationi

Entry nameiRHMD_ECODH
AccessioniPrimary (citable) accession number: B1X8W0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: May 20, 2008
Last modified: March 28, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health