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B1X8W0

- RHMD_ECODH

UniProt

B1X8W0 - RHMD_ECODH

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Protein

L-rhamnonate dehydratase

Gene

rhmD

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the dehydration of L-rhamnonate to 2-keto-3-deoxy-L-rhamnonate (KDR).UniRule annotation

Catalytic activityi

L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H2O.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331SubstrateUniRule annotation
Binding sitei59 – 591SubstrateUniRule annotation
Metal bindingi226 – 2261MagnesiumUniRule annotation
Metal bindingi252 – 2521MagnesiumUniRule annotation
Metal bindingi280 – 2801MagnesiumUniRule annotation
Sitei302 – 3021Increases basicity of active site HisUniRule annotation
Active sitei329 – 3291Proton acceptorUniRule annotation
Binding sitei349 – 3491SubstrateUniRule annotation
Sitei349 – 3491Transition state stabilizerUniRule annotation

GO - Molecular functioni

  1. L-rhamnonate dehydratase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular amino acid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL316385:GJ8B-2314-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-rhamnonate dehydrataseUniRule annotation (EC:4.2.1.90UniRule annotation)
Short name:
RhamDUniRule annotation
Gene namesi
Name:rhmDUniRule annotation
Ordered Locus Names:ECDH10B_2407
OrganismiEscherichia coli (strain K12 / DH10B)
Taxonomic identifieri316385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001689: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405L-rhamnonate dehydratasePRO_0000351692Add
BLAST

Proteomic databases

PRIDEiB1X8W0.

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi316385.ECDH10B_2407.

Structurei

3D structure databases

ProteinModelPortaliB1X8W0.
SMRiB1X8W0. Positions 1-405.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG4948.
HOGENOMiHOG000113755.
KOiK12661.
OMAiFCVELEA.
OrthoDBiEOG68Q0M0.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_01288. Rhamnon_dehydrat.
InterProiIPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR023444. L-Rhamnon_dehydrat.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view]
PANTHERiPTHR13794. PTHR13794. 1 hit.
PfamiPF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view]
SMARTiSM00922. MR_MLE. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEiPS00908. MR_MLE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B1X8W0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MENIMTLPKI KQVRAWFTGG ATAEKGAGGG DYHDQGANHW IDDHIATPMS
60 70 80 90 100
KYRDYEQSRQ SFGINVLGTL VVEVEAENGQ TGFAVSTAGE MGCFIVEKHL
110 120 130 140 150
NRFIEGKCVS DIKLIHDQML SATLYYSGSG GLVMNTISCV DLALWDLFGK
160 170 180 190 200
VVGLPVYKLL GGAVRDEIQF YATGARPDLA KEMGFIGGKM PTHWGPHDGD
210 220 230 240 250
AGIRKDAAMV ADMREKCGED FWLMLDCWMS QDVNYATKLA HACAPYNLKW
260 270 280 290 300
IEECLPPQQY ESYRELKRNA PVGMMVTSGE HHGTLQSFRT LSETGIDIMQ
310 320 330 340 350
PDVGWCGGLT TLVEIAAIAK SRGQLVVPHG SSVYSHHAVI TFTNTPFSEF
360 370 380 390 400
LMTSPDCSTM RPQFDPILLN EPVPVNGRIH KSVLDKPGFG VELNRDCNLK

RPYSH
Length:405
Mass (Da):44,714
Last modified:May 20, 2008 - v1
Checksum:i747D32E809A7B4DC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000948 Genomic DNA. Translation: ACB03407.1.
RefSeqiYP_001731185.1. NC_010473.1.

Genome annotation databases

EnsemblBacteriaiACB03407; ACB03407; ECDH10B_2407.
GeneIDi6058390.
KEGGiecd:ECDH10B_2407.
PATRICi18464683. VBIEscCol59506_2428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000948 Genomic DNA. Translation: ACB03407.1 .
RefSeqi YP_001731185.1. NC_010473.1.

3D structure databases

ProteinModelPortali B1X8W0.
SMRi B1X8W0. Positions 1-405.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 316385.ECDH10B_2407.

Proteomic databases

PRIDEi B1X8W0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB03407 ; ACB03407 ; ECDH10B_2407 .
GeneIDi 6058390.
KEGGi ecd:ECDH10B_2407.
PATRICi 18464683. VBIEscCol59506_2428.

Phylogenomic databases

eggNOGi COG4948.
HOGENOMi HOG000113755.
KOi K12661.
OMAi FCVELEA.
OrthoDBi EOG68Q0M0.

Enzyme and pathway databases

BioCyci ECOL316385:GJ8B-2314-MONOMER.

Family and domain databases

Gene3Di 3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPi MF_01288. Rhamnon_dehydrat.
InterProi IPR029065. Enolase_C-like.
IPR029017. Enolase_N_like.
IPR023444. L-Rhamnon_dehydrat.
IPR018110. Mandel_Rmase/mucon_lact_enz_CS.
IPR013342. Mandelate_racemase_C.
IPR013341. Mandelate_racemase_N_dom.
IPR001354. MR/MLE/MAL.
[Graphical view ]
PANTHERi PTHR13794. PTHR13794. 1 hit.
Pfami PF01188. MR_MLE. 1 hit.
PF02746. MR_MLE_N. 1 hit.
[Graphical view ]
SMARTi SM00922. MR_MLE. 1 hit.
[Graphical view ]
SUPFAMi SSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
PROSITEi PS00908. MR_MLE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse."
    Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R.
    J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / DH10B.

Entry informationi

Entry nameiRHMD_ECODH
AccessioniPrimary (citable) accession number: B1X8W0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: May 20, 2008
Last modified: October 29, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Reaction proceeds via a syn dehydration.UniRule annotation

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3