Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-lactate dehydrogenase

Gene

lldD

Organism
Escherichia coli (strain K12 / DH10B)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of L-lactate to pyruvate. Is coupled to the respiratory chain.UniRule annotation

Catalytic activityi

(S)-lactate + an oxidized electron acceptor = pyruvate + a reduced electron acceptor.UniRule annotation

Cofactori

FMNUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei24SubstrateUniRule annotation1
Binding sitei106FMNUniRule annotation1
Binding sitei127FMNUniRule annotation1
Binding sitei129SubstrateUniRule annotation1
Binding sitei155FMNUniRule annotation1
Binding sitei164SubstrateUniRule annotation1
Binding sitei251FMNUniRule annotation1
Active sitei275Proton acceptorUniRule annotation1
Binding sitei278SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi306 – 330FMNUniRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFlavoprotein, FMN

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.-.-UniRule annotation)
Gene namesi
Name:lldDUniRule annotation
Ordered Locus Names:ECDH10B_3787
OrganismiEscherichia coli (strain K12 / DH10B)
Taxonomic identifieri316385 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003834211 – 396L-lactate dehydrogenaseAdd BLAST396

Structurei

3D structure databases

ProteinModelPortaliB1X8M0.
SMRiB1X8M0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 380FMN hydroxy acid dehydrogenaseUniRule annotationAdd BLAST380

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DMF. Bacteria.
COG1304. LUCA.
HOGENOMiHOG000217464.
KOiK00101.
OMAiMQLYIYK.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01559. L_lact_dehydr. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
IPR020920. LldD.
PfamiView protein in Pfam
PF01070. FMN_dh. 1 hit.
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiView protein in PROSITE
PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.

Sequencei

Sequence statusi: Complete.

B1X8M0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIISAASDYR AAAQRILPPF LFHYMDGGAY SEYTLRRNVE DLSEVALRQR
60 70 80 90 100
ILKNMSDLSL ETTLFNEKLS MPVALAPVGL CGMYARRGEV QAAKAADAHG
110 120 130 140 150
IPFTLSTVSV CPIEEVAPAI KRPMWFQLYV LRDRGFMRNA LERAKAAGCS
160 170 180 190 200
TLVFTVDMPT PGARYRDAHS GMSGPNAAMR RYLQAVTHPQ WAWDVGLNGR
210 220 230 240 250
PHDLGNISAY LGKPTGLEDY IGWLGNNFDP SISWKDLEWI RDFWDGPMVI
260 270 280 290 300
KGILDPEDAR DAVRFGADGI VVSNHGGRQL DGVLSSARAL PAIADAVKGD
310 320 330 340 350
IAILADSGIR NGLDVVRMIA LGADTVLLGR AFLYALATAG QAGVANLLNL
360 370 380 390
IEKEMKVAMT LTGAKSISEI TQDSLVQGLG KELPAALAPM AKGNAA
Length:396
Mass (Da):42,728
Last modified:May 20, 2008 - v1
Checksum:iCA0D8E308713BF83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000948 Genomic DNA. Translation: ACB04655.1.
RefSeqiWP_000586962.1. NC_010473.1.

Genome annotation databases

EnsemblBacteriaiACB04655; ACB04655; ECDH10B_3787.
KEGGiecd:ECDH10B_3787.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLLDD_ECODH
AccessioniPrimary (citable) accession number: B1X8M0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 20, 2008
Last modified: July 5, 2017
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families