B1X8C6 (UBIG_ECODH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-demethylubiquinone-9 3-methyltransferase
EC=2.1.1.64

Alternative name(s):
2-polyprenyl-6-hydroxyphenyl methylase
EC=2.1.1.222
3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase
Short name=DHHB methyltransferase

Gene names

Name:	ubiG
Ordered Locus Names:	ECDH10B_2391

Organism

Escherichia coli (strain K12 / DH10B) [Complete proteome] [HAMAP]

Taxonomic identifier

316385 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli › Escherichia coli (strain K12)

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Non-specific O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway By similarity. HAMAP-Rule MF_00472
Catalytic activity	S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n. HAMAP-Rule MF_00472 S-adenosyl-L-methionine + 3-(all-trans-polyprenyl)benzene-1,2-diol = S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-polyprenyl)phenol. HAMAP-Rule MF_00472
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis. HAMAP-Rule MF_00472
Sequence similarities	Belongs to the methyltransferase superfamily. UbiG/COQ3 family.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	ubiquinone biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Inferred from electronic annotation. Source: InterPro 3-demethylubiquinone-9 3-O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	3-demethylubiquinone-9 3-methyltransferase HAMAP-Rule MF_00472		PRO_1000199678

Sequences

	Sequence		Length	Mass (Da)
	B1X8C6 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: D4EB4707A06F5613	FASTA	240	26,555
	10 20 30 40 50 60 MNAEKSPVNH NVDHEEIAKF EAVASRWWDL EGEFKPLHRI NPLRLGYIAE RAGGLFGKKV 70 80 90 100 110 120 LDVGCGGGIL AESMAREGAT VTGLDMGFEP LQVAKLHALE SGIQVDYVQE TVEEHAAKHA 130 140 150 160 170 180 GQYDVVTCME MLEHVPDPQS VVRACAQLVK PGGDVFFSTL NRNGKSWLMA VVGAEYILRM 190 200 210 220 230 240 VPKGTHDVKK FIKPAELLGW VDQTSLKERH ITGLHYNPIT NTFKLGPGVD VNYMLHTQNK

References

[1]

"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / DH10B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000948 Genomic DNA. Translation: ACB03392.1.
RefSeq	YP_001731170.1. NC_010473.1.
3D structure databases
ProteinModelPortal	B1X8C6.
SMR	B1X8C6. Positions 9-214.
ModBase	Search...
Protein-protein interaction databases
STRING	316385.ECDH10B_2391.
Proteomic databases
PRIDE	B1X8C6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACB03392; ACB03392; ECDH10B_2391.
GeneID	6058142.
KEGG	ecd:ECDH10B_2391.
PATRIC	18464649. VBIEscCol59506_2411.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2227.
HOGENOM	HOG000278065.
KO	K00568.
OMA	QETVESH.
ProtClustDB	PRK05134.
Enzyme and pathway databases
BioCyc	ECOL316385:GJ8B-2298-MONOMER.
UniPathway	UPA00232.
Family and domain databases
HAMAP	MF_00472. UbiG.
InterPro	IPR010233. UbiG_MeTrfase. [Graphical view]
PANTHER	PTHR23134. PTHR23134. 1 hit.
TIGRFAMs	TIGR01983. UbiG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UBIG_ECODH

Accession

Primary (citable) accession number: B1X8C6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program