Reviewed, UniProtKB/Swiss-Prot B1X7N2 (CDD_ECODH)

Names and origin

Protein names

Recommended name:
    Cytidine deaminase
    EC=3.5.4.5
Alternative name(s):
    Cytidine aminohydrolase
      Short name=CDA

Gene names

Name:	cdd
Ordered Locus Names:	ECDH10B_2300

Organism

Escherichia coli (strain DH10B) [Complete proteome] [HAMAP]

Taxonomic identifier

316385 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme scavenge exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis By similarity.
Catalytic activity	Cytidine + H2O = uridine + NH3. HAMAP MF_01558
Cofactor	Binds 1 zinc ion By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytidine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cytidine deaminase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Cytidine deaminase HAMAP MF_01558		PRO_1000147099
Regions
	Region	89 – 91	3	Substrate binding By similarity
Sites
	Active site	104	1	Proton donor By similarity
	Metal binding	102	1	Zinc; catalytic By similarity
	Metal binding	129	1	Zinc; catalytic By similarity
	Metal binding	132	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1X7N2-1 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: F0B5CD68AB145D7D	FASTA	294	31,540
	10 20 30 40 50 60 MHPRFQTAFA QLADNLQSAL EPILADKYFP ALLTGEQVSS LKSATGLDED ALAFALLPLA 70 80 90 100 110 120 AACARTPLSN FNVGAIARGV SGTWYFGANM EFIGATMQQT VHAEQSAISH AWLSGEKALA 130 140 150 160 170 180 AITVNYTPCG HCRQFMNELN SGLDLRIHLP GREAHALRDY LPDAFGPKDL EIKTLLMDEQ 190 200 210 220 230 240 DHGYALTGDA LSQAAIAAAN RSHMPYSKSP SGVALECKDG RIFSGSYAEN AAFNPTLPPL 250 260 270 280 290 QGALILLNLK GYDYPDIQRA VLAEKADAPL IQWDATSATL KALGCHSIDR VLLA

References

[1]

"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008) [PubMed: 18245285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000948 Genomic DNA. Translation: ACB03308.1.
RefSeq	YP_001731086.1.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	B1X7N2.
Genome annotation databases
GeneID	6060167.
GenomeReviews	Gene locus ECDH10B_2300 in contig CP000948_GR.
KEGG	ecd:ECDH10B_2300.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FSPCGHC.
Family and domain databases
HAMAP	MF_01558. [Tree]
InterPro	IPR016192. APOBEC/CMP_deaminase_Zn-bd. IPR002125. CMP_dCMP_Zn_bd. IPR013171. Cyd/dCyd_deaminase_Zn_bd. IPR006263. Cyt_deam_dimer. [Graphical view]
Pfam	PF00383. dCMP_cyt_deam_1. 1 hit. PF08211. dCMP_cyt_deam_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01355. cyt_deam_dimer. 1 hit.
PROSITE	PS00903. CYT_DCMP_DEAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDD_ECODH

Accession

Primary (citable) accession number: B1X7N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)