B1X797 (6PGL_ECODH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphogluconolactonase
Short name=6-P-gluconolactonase
EC=3.1.1.31

Gene names

Name:	pgl
Ordered Locus Names:	ECDH10B_0835

Organism

Escherichia coli (strain K12 / DH10B) [Complete proteome] [HAMAP]

Taxonomic identifier

316385 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate By similarity. HAMAP MF_01605
Catalytic activity	6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate. HAMAP MF_01605
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3. HAMAP MF_01605
Sequence similarities	Belongs to the cycloisomerase 2 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Molecular function	Hydrolase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphogluconolactonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	6-phosphogluconolactonase HAMAP MF_01605		PRO_1000148154
Amino acid modifications
	Modified residue	287	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B1X797 [UniParc]. Last modified May 20, 2008. Version 1. Checksum: D731044CFCF31A8F	FASTA	331	36,308
	10 20 30 40 50 60 MKQTVYIASP ESQQIHVWNL NHEGALTLTQ VVDVPGQVQP MVVSPDKRYL YVGVRPEFRV 70 80 90 100 110 120 LAYRIAPDDG ALTFAAESAL PGSPTHISTD HQGQFVFVGS YNAGNVSVTR LEDGLPVGVV 130 140 150 160 170 180 DVVEGLDGCH SANISPDNRT LWVPALKQDR ICLFTVSDDG HLVAQDPAEV TTVEGAGPRH 190 200 210 220 230 240 MVFHPNEQYA YCVNELNSSV DVWELKDPHG NIECVQTLDM MPENFSDTRW AADIHITPDG 250 260 270 280 290 300 RHLYACDRTA SLITVFSVSE DGSVLSKEGF QPTETQPRGF NVDHSGKYLI AAGQKSHHIS 310 320 330 VYEIVGEQGL LHEKGRYAVG QGPMWVVVNA H

References

[1]

"The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse." Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B., Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B., Posfai G., Weinstock G.M., Blattner F.R. J. Bacteriol. 190:2597-2606(2008) [PubMed: 18245285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / DH10B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000948 Genomic DNA. Translation: ACB01968.1.
RefSeq	YP_001729746.1. NC_010473.1.
3D structure databases
ProteinModelPortal	B1X797.
SMR	B1X797. Positions 1-331.
ModBase	Search...
Protein-protein interaction databases
STRING	B1X797.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000012250; EBESCP00000011778; EBESCG00000011312.
GeneID	6061170.
GenomeReviews	Gene locus ECDH10B_0835 in contig CP000948_GR.
KEGG	ecd:ECDH10B_0835.
PATRIC	18461478. VBIEscCol59506_0841.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000009586.
HOGENOM	HBG741123.
OMA	CINELNS.
ProtClustDB	PRK11028.
Enzyme and pathway databases
BioCyc	ECOL316385:ECDH10B_0835-MONOMER.
Family and domain databases
HAMAP	MF_01605. 6P-gluconolactonase. [Tree]
InterPro	IPR022528. 6-phosphogluconolactonase_YbhE. IPR019405. Lactonase_7-beta_prop. IPR011045. N2O_reductase_N. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K07404.
Pfam	PF10282. Lactonase. 1 hit. [Graphical view]
SUPFAM	SSF50974. N2O_reductase_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

6PGL_ECODH

Accession

Primary (citable) accession number: B1X797

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 20, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program