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B1X4X1 (LIPA_PAUCH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase, organellar chromatophore

EC=2.8.1.8
Alternative name(s):
Lipoate synthase
Short name=LS
Short name=Lip-syn
Lipoic acid synthase
Gene names
Ordered Locus Names:PCC_0559
Encoded onPlastid; Organellar chromatophore
OrganismPaulinella chromatophora
Taxonomic identifier39717 [NCBI]
Taxonomic lineageEukaryotaRhizariaCercozoaSilicofiloseaEuglyphidaPaulinellidaePaulinella

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03123

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03123

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03123

Subcellular location

Plastidorganellar chromatophore HAMAP-Rule MF_03123.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Lipoyl synthase, organellar chromatophore HAMAP-Rule MF_03123
PRO_0000398235

Sites

Metal binding331Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding381Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding441Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding591Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding631Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding661Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
B1X4X1 [UniParc].

Last modified May 20, 2008. Version 1.
Checksum: C3D08984BC910713

FASTA29132,395
        10         20         30         40         50         60 
MLKPTWLRVK APQKERIGTI NRLLTDLKLN TVCEEASCPN IGECFAGGTA TFLIMGPGCT 

        70         80         90        100        110        120 
RACPYCDIDF DKSIRTVDHT EPTRIGEAVS RMRLKHVVIT SVNRDDLADG GASHFVACIA 

       130        140        150        160        170        180 
AVRAASPTTT IEVLIPDFCG NLNSIDKVLA AKPEIINHNI ETVPRIYNRV RPQGVYQRSL 

       190        200        210        220        230        240 
YLLDWIKSND KNLYTKSGLM VGLGEIDAEV LEVMADLRAI QVDVVTIGQY LSPGSKHLPI 

       250        260        270        280        290 
DRFVTPTQFD NYKAIGELEM NFLQVVSTPL TRSSYHSGEF RNLMRDNPRQ N 

« Hide

References

[1]"Chromatophore genome sequence of Paulinella sheds light on acquisition of photosynthesis by eukaryotes."
Nowack E.C.M., Melkonian M., Gloeckner G.
Curr. Biol. 18:410-418(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000815 Genomic DNA. Translation: ACB42990.1.
RefSeqYP_002049200.1. NC_011087.1.

3D structure databases

ProteinModelPortalB1X4X1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6481678.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA_PAUCH
AccessionPrimary (citable) accession number: B1X4X1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 20, 2008
Last modified: February 19, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways