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Protein

Lipoyl synthase, organellar chromatophore

Gene

PCC_0559

Organism
Paulinella chromatophora
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable octanoyltransferase (lipB)
  2. Lipoyl synthase, organellar chromatophore (PCC_0559)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi33Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi38Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi44Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi59Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi63Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi66Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, organellar chromatophoreUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Ordered Locus Names:PCC_0559
Encoded oniPlastid; Organellar chromatophore
OrganismiPaulinella chromatophora
Taxonomic identifieri39717 [NCBI]
Taxonomic lineageiEukaryotaRhizariaCercozoaImbricateaSilicofiloseaEuglyphidaPaulinellidaePaulinella

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Organellar chromatophore, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003982351 – 291Lipoyl synthase, organellar chromatophoreAdd BLAST291

Structurei

3D structure databases

ProteinModelPortaliB1X4X1
SMRiB1X4X1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

B1X4X1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKPTWLRVK APQKERIGTI NRLLTDLKLN TVCEEASCPN IGECFAGGTA
60 70 80 90 100
TFLIMGPGCT RACPYCDIDF DKSIRTVDHT EPTRIGEAVS RMRLKHVVIT
110 120 130 140 150
SVNRDDLADG GASHFVACIA AVRAASPTTT IEVLIPDFCG NLNSIDKVLA
160 170 180 190 200
AKPEIINHNI ETVPRIYNRV RPQGVYQRSL YLLDWIKSND KNLYTKSGLM
210 220 230 240 250
VGLGEIDAEV LEVMADLRAI QVDVVTIGQY LSPGSKHLPI DRFVTPTQFD
260 270 280 290
NYKAIGELEM NFLQVVSTPL TRSSYHSGEF RNLMRDNPRQ N
Length:291
Mass (Da):32,395
Last modified:May 20, 2008 - v1
Checksum:iC3D08984BC910713
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000815 Genomic DNA Translation: ACB42990.1
RefSeqiYP_002049200.1, NC_011087.1

Genome annotation databases

EnsemblProtistsiACB42990; ACB42990; PCC_0559
GeneIDi6481678

Similar proteinsi

Entry informationi

Entry nameiLIPA_PAUCH
AccessioniPrimary (citable) accession number: B1X4X1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 20, 2008
Last modified: May 23, 2018
This is version 44 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

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