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B1X4X1

- LIPA_PAUCH

UniProt

B1X4X1 - LIPA_PAUCH

Protein

Lipoyl synthase, organellar chromatophore

Gene

PCC_0559

Organism
Paulinella chromatophora
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (20 May 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi33 – 331Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi38 – 381Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi44 – 441Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi59 – 591Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi63 – 631Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi66 – 661Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, organellar chromatophoreUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Ordered Locus Names:PCC_0559
    Encoded oniPlastid; Organellar chromatophore
    OrganismiPaulinella chromatophora
    Taxonomic identifieri39717 [NCBI]
    Taxonomic lineageiEukaryotaRhizariaCercozoaSilicofiloseaEuglyphidaPaulinellidaePaulinella

    Subcellular locationi

    GO - Cellular componenti

    1. organellar chromatophore Source: UniProtKB-SubCell
    2. plastid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Organellar chromatophore, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291Lipoyl synthase, organellar chromatophorePRO_0000398235Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliB1X4X1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B1X4X1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKPTWLRVK APQKERIGTI NRLLTDLKLN TVCEEASCPN IGECFAGGTA    50
    TFLIMGPGCT RACPYCDIDF DKSIRTVDHT EPTRIGEAVS RMRLKHVVIT 100
    SVNRDDLADG GASHFVACIA AVRAASPTTT IEVLIPDFCG NLNSIDKVLA 150
    AKPEIINHNI ETVPRIYNRV RPQGVYQRSL YLLDWIKSND KNLYTKSGLM 200
    VGLGEIDAEV LEVMADLRAI QVDVVTIGQY LSPGSKHLPI DRFVTPTQFD 250
    NYKAIGELEM NFLQVVSTPL TRSSYHSGEF RNLMRDNPRQ N 291
    Length:291
    Mass (Da):32,395
    Last modified:May 20, 2008 - v1
    Checksum:iC3D08984BC910713
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000815 Genomic DNA. Translation: ACB42990.1.
    RefSeqiYP_002049200.1. NC_011087.1.

    Genome annotation databases

    GeneIDi6481678.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000815 Genomic DNA. Translation: ACB42990.1 .
    RefSeqi YP_002049200.1. NC_011087.1.

    3D structure databases

    ProteinModelPortali B1X4X1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 6481678.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Chromatophore genome sequence of Paulinella sheds light on acquisition of photosynthesis by eukaryotes."
      Nowack E.C.M., Melkonian M., Gloeckner G.
      Curr. Biol. 18:410-418(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiLIPA_PAUCH
    AccessioniPrimary (citable) accession number: B1X4X1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: May 20, 2008
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3